BBa_K582000
1
BBa_K582000
500bp of Spacer/Repeat Region of the CRISPR genome present in Strep. thermophilus LMD9 strain
2011-09-27T11:00:00Z
2015-05-08T01:12:46Z
http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=fasta&from=1379972&to=1384138
This comes from the genomic sequence of Streptococcus Thermophilus LMD-9
The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system on a shuttling vector with the Spacer/Repeat region, we hope that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous.
false
false
_753_
0
7155
9
It's complicated
true
So, the entire Repeat/Spacer Region is 1400bp but since it had a SpeI site at 500 bp and we were unable to mutate it. So, we cut it at the 500bp and just used that our part.
false
Kettner Griswold, Mitesh Agrawal
BBa_K582000_sequence
1
accttatttattgacaaaaatgctgagacaacctagtctctcacttgttgattcagtaatattgaatatcctaaatcagttgtttcattttagttaccgtataagatatttacaaaaatctgatgaaaaacttttacagaaatttttagaaagtaaggattgacaagaacagttattgattttataatcactatgtgggtatgaaaatctcaaaaatcatttgaggtttttgtactctcaagatttaagtaactgtacaacatgatgatgaagtatcgtcatctactaacgtttttgtactctcaagatttaagtaactgtacaaccttcacctcaaatcttagagctggactaaagtttttgtactctcaagatttaagtaactgtacaacatgtctgaaaaataaccgaccatcattactgtttttgtactctcaagatttaagtaactgtacaacgaagctcatcatgttaaggctaaaacctatgtttttgtactctcaagatttaagtaactgtacaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z