BBa_K582002 1 BBa_K582002 Complete Repeat/Spacer Region(1400 bp) of LMD 9 2011-09-27T11:00:00Z 2015-05-08T01:12:46Z http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141 for Cas9 http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=genbank&from=648900&to=650325 for Repeat/Spacer Region The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system and sigma factor 70, we hypothesize that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous. false false _753_ 0 7155 9 Not in stock false The sequence has the restriction sites present on the pSB1C3 backbone. We will have to subject it to mutagenesis to make the biobrick functional. false Mitesh Agrawal BBa_K582001 1 BBa_K582001 Cas9 genome from the Streptococcus Thermophilus LMD-9 strain 2011-09-27T11:00:00Z 2015-05-08T01:12:46Z http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141 Cas9 genome is a part of the CRISPR sequence existing in the Strep. Thermophilus LMD-9 strain. As referred by the Barrangou 2011 paper, cas9 is the only essential cas protein required for the CRISPR mechanism to express. We propose a biobrick with the cas9 site present in it as well as the promoter sigma factor 70 for its expression. false false _753_ 0 7155 9 Not in stock false We had to mutate the restriction sites present in the cas9 sequence which are present in the biobrick. false Mitesh Agrawal BBa_K582003 1 BBa_K582003 Cas 9 + Repeat/Spacer Region 2011-09-27T11:00:00Z 2015-05-08T01:12:46Z http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141 for Cas9 http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=genbank&from=648900&to=650325 for Repeat/Spacer Region The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system and sigma factor 70, we hypothesize that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous. false false _753_ 0 7155 9 Not in stock false The sequence has the restriction sites present on the pSB1C3 backbone. We will have to subject it to mutagenesis to make the biobrick functional. false Mitesh Agrawal component2146680 1 BBa_K582001 component2146681 1 BBa_K582002 annotation2146681 1 BBa_K582002 range2146681 1 4176 5601 annotation2146680 1 BBa_K582001 range2146680 1 1 4167 BBa_K582003_sequence 1 atgactaagccatactcaattggacttgatattggaacgaatagtgttggatgggctgtaacaactgataattacaaggttccgtctaaaaaaatgaaagtcttaggaaatacgagtaaaaagtatatcaaaaagaacctgttaggtgtattactctttgactctggaatcacagcagaaggaagaagattgaagcgtactgcaagaagacgttatactagacgccgtaatcgtatcctttatttgcaggaaatttttagcacagagatggctacattagatgatgctttctttcaaagacttgacgattcgtttttagttcctgatgataaacgtgatagtaagtatccgatatttggaaacttagtagaagaaaaagcctatcatgatgaatttccaactatctatcatttaaggaaatatttagcagatagtactaaaaaagcagatttgcgtctagtttatcttgcattggctcatatgattaaatatagaggtcacttcttaattgaaggagagtttaattcaaaaaataatgatattcagaagaattttcaagactttttggacacttataatgctatttttgaatcggatttatcacttgagaatagtaaacaacttgaggaaattgttaaagataagattagtaaattagaaaagaaagatcgtattttaaaactcttccctggggagaaaaactcggggattttttcagagtttctaaagttgattgtaggaaatcaagctgattttaggaaatgttttaatttagacgaaaaagcctccttacatttttccaaagaaagctatgatgaagatttagagactttgttaggttatattggagatgattacagtgatgtctttctcaaagcaaagaaactttatgatgctattcttttatcgggttttctgactgtaactgataatgagacagaagcacctctctcttctgctatgataaagcgatataatgaacacaaagaagatttagcgttactaaaggaatatataagaaatatttcactaaaaacgtataatgaagtatttaaagatgacaccaaaaatggttatgctggttatattgatggaaaaacaaatcaggaagatttctacgtatatctaaaaaaactattggctgaatttgaaggtgcggattattttcttgaaaaaattgatcgagaagattttttgagaaagcaacgtacatttgacaatggttcgataccatatcagattcatcttcaagaaatgagagcaattcttgataagcaagctaaattttatcctttcttggctaaaaataaagaaagaatcgagaagattttaaccttccggataccttattatgtaggtccacttgcgagagggaatagtgattttgcctggtcaataagaaaacgaaatgaaaaaattacaccttggaattttgaggacgttattgacaaagaatcttcggcagaggccttcattaatcgaatgacgagctttgatttgtatttgccagaagagaaggtacttccaaagcatagtctcttatacgaaacttttaatgtatataatgaattaacaaaagttagatttattgccgaaagtatgagagattatcaatttttagatagtaagcagaagaaagatattgttagactttattttaaagataaaaggaaagttactgataaggatattattgaatatttacatgcaatttatgggtatgatggaattgaattaaaaggcatagagaaacagtttaattctagtttatctacttatcacgatcttttaaatattattaatgataaagagtttttggatgatagttcaaatgaagcgattatcgaagaaattatccatactttgacaatttttgaagatagagagatgataaaacaacgtctttcaaaatttgagaatatattcgataaatccgttttgaaaaagttatctcgtagacattacactggctggggtaagttatctgctaagcttattaatggtattcgagatgaaaaatctggtaatactattcttgattacttaattgatgatggtatttctaaccgtaatttcatgcaacttattcacgatgatgctctttcttttaaaaagaagatacagaaagcacaaattattggtgacgaagataaaggtaatattaaagaggtcgttaagtctttgccaggtagtcctgcgattaaaaaaggtattttacaaagcataaaaattgtagatgaattggtcaaagtaatgggaggaagaaaacccgagtcaattgttgttgagatggctcgtgaaaatcaatataccaatcaaggtaagtctaattcccaacaacgcttgaaacgtttagaaaaatctctcaaagagttaggtagtaagatacttaaggaaaatattcctgcaaaactttctaaaatagacaataacgcacttcaaaatgatcgactttacttatactatcttcaaaatggaaaagatatgtataccggagatgatttagatattgatagattaagtaattatgatattgatcatattattcctcaagcttttttgaaagataattctattgacaataaagtacttgtttcatctgctagtaaccgtggtaaatcagatgatgttccaagtttagaggttgtcaaaaaaagaaagacattttggtatcaattattgaaatcaaaattaatttctcaacgaaaatttgataatctgacaaaagctgaacggggaggattgtcacctgaggacaaagctggttttattcaacgccagttggttgaaacacgtcaaataacaaaacatgtagctcgtttacttgatgagaaatttaataataaaaaagatgaaaataatagagcggtacgaacagtaaaaattattaccttgaaatctaccttagtttctcaatttcgtaaggattttgaactttataaagttcgtgaaatcaatgattttcatcatgctcatgatgcttacttgaatgccgttgtagcaagtgctttacttaagaaataccctaaactagagccagaatttgtgtacggtgattatccaaaatacaatagttttagagaaagaaagtccgctacagaaaaggtatatttctattcaaatatcatgaatatctttaaaaaatctatttctttagctgatggtagagttattgaaagaccacttattgaggtaaatgaggagaccggcgaatccgtttggaataaagaatctgatttagcaactgtaaggagagtactctcttatccgcaagtaaatgttgtgaaaaaagttgaggaacagaatcacggattggatagaggaaaaccaaagggattgtttaatgcaaatctttcctcaaagccaaaaccaaatagtaatgaaaatttagtaggtgctaaagagtatcttgaccccaaaaagtatggggggtatgctggaatttctaattcttttactgttcttgttaaagggacaattgaaaaaggtgctaagaaaaaaataacaaatgtactagaatttcaaggtatttctattttagataggattaattatagaaaagataaacttaattttttacttgaaaaaggttataaagatattgagttaattattgaactacctaaatatagtttatttgaactttcagatggttcacgtcgtatgttggctagtattttgtcaacgaataataagaggggagagattcacaaaggaaatcagatttttctttcacagaagtttgtgaaattactttatcatgctaagagaataagtaacacaattaatgagaatcatagaaaatatgttgagaaccataaaaaagagtttgaagaattattttactacattcttgagtttaatgagaattatgttggagctaaaaagaatggtaaactcttaaactctgcctttcaatcttggcaaaatcatagtatagatgaactctgtagtagttttataggacctaccggaagtgaaagaaaggggctatttgaattaacctctcgtggaagtgctgctgattttgaatttttaggtgttaaaattccaaggtatagagactataccccatcatccctattaaaagatgccacacttattcatcaatctgttacaggcctctatgaaacacgaatagaccttgccaaactaggagagggttaatactagagaccttatttattgacaaaaatgctgagacaacctagtctctcacttgttgattcagtaatattgaatatcctaaatcagttgtttcattttagttaccgtataagatatttacaaaaatctgatgaaaaacttttacagaaatttttagaaagtaaggattgacaagaacagttattgattttataatcactatgtgggtatgaaaatctcaaaaatcatttgaggtttttgtactctcaagatttaagtaactgtacaacatgatgatgaagtatcgtcatctactaacgtttttgtactctcaagatttaagtaactgtacaaccttcacctcaaatcttagagctggactaaagtttttgtactctcaagatttaagtaactgtacaacatgtctgaaaaataaccgaccatcattactgtttttgtactctcaagatttaagtaactgtacaacgaagctcatcatgttaaggctaaaacctatgtttttgtactctcaagatttaagtaactgtacaactagtctaaatagatttcttgcaccattgtagtttttgtactctcaagatttaagtaactgtacaacattcgtgaaaaaatatcgtgaaataggcaagtttttgtactctcaagatttaagtaactgtacaactctaggctcatctaaagataaatcagtagcgtttttgtactctcaagatttaagtaactgtacaactaaaaacatggggcggcggtaatagtgtaaggtttttgtactctcaagatttaagtaactgtacaacacaaccagcaaagagagcgccgacaacattgtttttgtactctcaagatttaagtaactgtacaactataacacaggtttagaggatgttatacttgtttttgtactctcaagatttaagtaactgtacaacctagaagctcaagcggtaaaagttgatggcggtttttgtactctcaagatttaagtaactgtacaacctttgagggcaagccctcgccgttccatttgtttttgtactctcaagatttaagtaactgtacaacaactaccaagcaaatcagcaatcaataagtgtttttgtactctcaagatttaagtaactgtacaacctataagtgacaatcagcgtagggaatacggtttttgtactctcaagatttaagtaactgtacaacatcagtgcggtatatttaccctagacgctagtttttgtactctcaagatttaagtaactgtacaacaacagttactattaatcacgattccaacgggtttttgtactctcaagatttaagtaactgtacagtttgattcaacataaaaagccggttcaattgaacttggctttttaaaatacacgataaacataaggattgtcaggctgactaacctctttaacctcagtcaaattaagg BBa_K582001_sequence 1 atgactaagccatactcaattggacttgatattggaacgaatagtgttggatgggctgtaacaactgataattacaaggttccgtctaaaaaaatgaaagtcttaggaaatacgagtaaaaagtatatcaaaaagaacctgttaggtgtattactctttgactctggaatcacagcagaaggaagaagattgaagcgtactgcaagaagacgttatactagacgccgtaatcgtatcctttatttgcaggaaatttttagcacagagatggctacattagatgatgctttctttcaaagacttgacgattcgtttttagttcctgatgataaacgtgatagtaagtatccgatatttggaaacttagtagaagaaaaagcctatcatgatgaatttccaactatctatcatttaaggaaatatttagcagatagtactaaaaaagcagatttgcgtctagtttatcttgcattggctcatatgattaaatatagaggtcacttcttaattgaaggagagtttaattcaaaaaataatgatattcagaagaattttcaagactttttggacacttataatgctatttttgaatcggatttatcacttgagaatagtaaacaacttgaggaaattgttaaagataagattagtaaattagaaaagaaagatcgtattttaaaactcttccctggggagaaaaactcggggattttttcagagtttctaaagttgattgtaggaaatcaagctgattttaggaaatgttttaatttagacgaaaaagcctccttacatttttccaaagaaagctatgatgaagatttagagactttgttaggttatattggagatgattacagtgatgtctttctcaaagcaaagaaactttatgatgctattcttttatcgggttttctgactgtaactgataatgagacagaagcacctctctcttctgctatgataaagcgatataatgaacacaaagaagatttagcgttactaaaggaatatataagaaatatttcactaaaaacgtataatgaagtatttaaagatgacaccaaaaatggttatgctggttatattgatggaaaaacaaatcaggaagatttctacgtatatctaaaaaaactattggctgaatttgaaggtgcggattattttcttgaaaaaattgatcgagaagattttttgagaaagcaacgtacatttgacaatggttcgataccatatcagattcatcttcaagaaatgagagcaattcttgataagcaagctaaattttatcctttcttggctaaaaataaagaaagaatcgagaagattttaaccttccggataccttattatgtaggtccacttgcgagagggaatagtgattttgcctggtcaataagaaaacgaaatgaaaaaattacaccttggaattttgaggacgttattgacaaagaatcttcggcagaggccttcattaatcgaatgacgagctttgatttgtatttgccagaagagaaggtacttccaaagcatagtctcttatacgaaacttttaatgtatataatgaattaacaaaagttagatttattgccgaaagtatgagagattatcaatttttagatagtaagcagaagaaagatattgttagactttattttaaagataaaaggaaagttactgataaggatattattgaatatttacatgcaatttatgggtatgatggaattgaattaaaaggcatagagaaacagtttaattctagtttatctacttatcacgatcttttaaatattattaatgataaagagtttttggatgatagttcaaatgaagcgattatcgaagaaattatccatactttgacaatttttgaagatagagagatgataaaacaacgtctttcaaaatttgagaatatattcgataaatccgttttgaaaaagttatctcgtagacattacactggctggggtaagttatctgctaagcttattaatggtattcgagatgaaaaatctggtaatactattcttgattacttaattgatgatggtatttctaaccgtaatttcatgcaacttattcacgatgatgctctttcttttaaaaagaagatacagaaagcacaaattattggtgacgaagataaaggtaatattaaagaggtcgttaagtctttgccaggtagtcctgcgattaaaaaaggtattttacaaagcataaaaattgtagatgaattggtcaaagtaatgggaggaagaaaacccgagtcaattgttgttgagatggctcgtgaaaatcaatataccaatcaaggtaagtctaattcccaacaacgcttgaaacgtttagaaaaatctctcaaagagttaggtagtaagatacttaaggaaaatattcctgcaaaactttctaaaatagacaataacgcacttcaaaatgatcgactttacttatactatcttcaaaatggaaaagatatgtataccggagatgatttagatattgatagattaagtaattatgatattgatcatattattcctcaagcttttttgaaagataattctattgacaataaagtacttgtttcatctgctagtaaccgtggtaaatcagatgatgttccaagtttagaggttgtcaaaaaaagaaagacattttggtatcaattattgaaatcaaaattaatttctcaacgaaaatttgataatctgacaaaagctgaacggggaggattgtcacctgaggacaaagctggttttattcaacgccagttggttgaaacacgtcaaataacaaaacatgtagctcgtttacttgatgagaaatttaataataaaaaagatgaaaataatagagcggtacgaacagtaaaaattattaccttgaaatctaccttagtttctcaatttcgtaaggattttgaactttataaagttcgtgaaatcaatgattttcatcatgctcatgatgcttacttgaatgccgttgtagcaagtgctttacttaagaaataccctaaactagagccagaatttgtgtacggtgattatccaaaatacaatagttttagagaaagaaagtccgctacagaaaaggtatatttctattcaaatatcatgaatatctttaaaaaatctatttctttagctgatggtagagttattgaaagaccacttattgaggtaaatgaggagaccggcgaatccgtttggaataaagaatctgatttagcaactgtaaggagagtactctcttatccgcaagtaaatgttgtgaaaaaagttgaggaacagaatcacggattggatagaggaaaaccaaagggattgtttaatgcaaatctttcctcaaagccaaaaccaaatagtaatgaaaatttagtaggtgctaaagagtatcttgaccccaaaaagtatggggggtatgctggaatttctaattcttttactgttcttgttaaagggacaattgaaaaaggtgctaagaaaaaaataacaaatgtactagaatttcaaggtatttctattttagataggattaattatagaaaagataaacttaattttttacttgaaaaaggttataaagatattgagttaattattgaactacctaaatatagtttatttgaactttcagatggttcacgtcgtatgttggctagtattttgtcaacgaataataagaggggagagattcacaaaggaaatcagatttttctttcacagaagtttgtgaaattactttatcatgctaagagaataagtaacacaattaatgagaatcatagaaaatatgttgagaaccataaaaaagagtttgaagaattattttactacattcttgagtttaatgagaattatgttggagctaaaaagaatggtaaactcttaaactctgcctttcaatcttggcaaaatcatagtatagatgaactctgtagtagttttataggacctaccggaagtgaaagaaaggggctatttgaattaacctctcgtggaagtgctgctgattttgaatttttaggtgttaaaattccaaggtatagagactataccccatcatccctattaaaagatgccacacttattcatcaatctgttacaggcctctatgaaacacgaatagaccttgccaaactaggagagggttaa BBa_K582002_sequence 1 accttatttattgacaaaaatgctgagacaacctagtctctcacttgttgattcagtaatattgaatatcctaaatcagttgtttcattttagttaccgtataagatatttacaaaaatctgatgaaaaacttttacagaaatttttagaaagtaaggattgacaagaacagttattgattttataatcactatgtgggtatgaaaatctcaaaaatcatttgaggtttttgtactctcaagatttaagtaactgtacaacatgatgatgaagtatcgtcatctactaacgtttttgtactctcaagatttaagtaactgtacaaccttcacctcaaatcttagagctggactaaagtttttgtactctcaagatttaagtaactgtacaacatgtctgaaaaataaccgaccatcattactgtttttgtactctcaagatttaagtaactgtacaacgaagctcatcatgttaaggctaaaacctatgtttttgtactctcaagatttaagtaactgtacaactagtctaaatagatttcttgcaccattgtagtttttgtactctcaagatttaagtaactgtacaacattcgtgaaaaaatatcgtgaaataggcaagtttttgtactctcaagatttaagtaactgtacaactctaggctcatctaaagataaatcagtagcgtttttgtactctcaagatttaagtaactgtacaactaaaaacatggggcggcggtaatagtgtaaggtttttgtactctcaagatttaagtaactgtacaacacaaccagcaaagagagcgccgacaacattgtttttgtactctcaagatttaagtaactgtacaactataacacaggtttagaggatgttatacttgtttttgtactctcaagatttaagtaactgtacaacctagaagctcaagcggtaaaagttgatggcggtttttgtactctcaagatttaagtaactgtacaacctttgagggcaagccctcgccgttccatttgtttttgtactctcaagatttaagtaactgtacaacaactaccaagcaaatcagcaatcaataagtgtttttgtactctcaagatttaagtaactgtacaacctataagtgacaatcagcgtagggaatacggtttttgtactctcaagatttaagtaactgtacaacatcagtgcggtatatttaccctagacgctagtttttgtactctcaagatttaagtaactgtacaacaacagttactattaatcacgattccaacgggtttttgtactctcaagatttaagtaactgtacagtttgattcaacataaaaagccggttcaattgaacttggctttttaaaatacacgataaacataaggattgtcaggctgactaacctctttaacctcagtcaaattaagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z