LOCUS       BBa_K584002              958 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac-Lux hybrid + GFP generator
ACCESSION   BBa_K584002
VERSION     BBa_K584002.1  
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /label=-35
     misc_feature    34..39
                     /label=-10
     misc_feature    16..33
                     /label=lux box
     misc_feature    40..74
                     /label=lacI binding site
     promoter        1..74
                     /label=BBa_K091100
     RBS             83..95
                     /label=RBS-3\Weak
     RBS             89..92
                     /label=RBS
     misc_feature    83..95
                     /label=BBa_B0032
     RBS             83..95
                     /label=BBa_B0032
     CDS             102..821
                     /label=GFP protein
     CDS             102..821
                     /label=BBa_E0040
     stem_loop       841..884
                     /label=stem_loop
     misc_feature    830..909
                     /label=BBa_B0010
     terminator      830..909
                     /label=BBa_B0010
     stem_loop       925..944
                     /label=T7 TE
     misc_feature    951..951
                     /label=stop
     polyA_site      945..958
                     /label=polya
     misc_feature    918..958
                     /label=BBa_B0012
     terminator      918..958
                     /label=BBa_B0012
     misc_feature    83..958
                     /label=BBa_E0240
ORIGIN
        1 accccaggct tgacacctgt aggatcgtac aggtataatt gtgtggaatt gtgagcggat
       61 aacaatttca cacatactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac
      121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      181 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
      301 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
      361 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      421 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      481 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      541 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      601 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      721 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
      781 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      901 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//