LOCUS       BBa_K584003              981 bp    DNA     linear       22-Dec-2024
DEFINITION  Lambda cI and LuxR regulated - hybrid promoter + GFP generator
ACCESSION   BBa_K584003
VERSION     BBa_K584003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     RBS             106..118
                     /label=RBS-3\Weak
     RBS             112..115
                     /label=RBS
     misc_feature    106..118
                     /label=BBa_B0032
     RBS             106..118
                     /label=BBa_B0032
     CDS             125..844
                     /label=GFP protein
     CDS             125..844
                     /label=BBa_E0040
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    106..981
                     /label=BBa_E0240
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagtcaca caggaaagta
      121 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      181 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      241 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      301 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      361 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      421 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      481 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      541 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      601 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      661 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      721 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      781 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
      841 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat a
//