LOCUS       BBa_K584004             1277 bp    DNA     linear       25-Nov-2024
DEFINITION  HybB promoter + GFP generator
ACCESSION   BBa_K584004
VERSION     BBa_K584004.1  
FEATURES             Location/Qualifiers
     promoter        1..393
                     /label=BBa_J45503
     RBS             402..414
                     /label=RBS-3\Weak
     RBS             408..411
                     /label=RBS
     misc_feature    402..414
                     /label=BBa_B0032
     RBS             402..414
                     /label=BBa_B0032
     CDS             421..1140
                     /label=GFP protein
     CDS             421..1140
                     /label=BBa_E0040
     stem_loop       1160..1203
                     /label=stem_loop
     misc_feature    1149..1228
                     /label=BBa_B0010
     terminator      1149..1228
                     /label=BBa_B0010
     stem_loop       1244..1263
                     /label=T7 TE
     misc_feature    1270..1270
                     /label=stop
     polyA_site      1264..1277
                     /label=polya
     misc_feature    1237..1277
                     /label=BBa_B0012
     terminator      1237..1277
                     /label=BBa_B0012
     misc_feature    402..1277
                     /label=BBa_E0240
ORIGIN
        1 cgccgctatg gactggataa agagatggta atggatttct ttcgtgagaa taattcctgt
       61 tctacgttgc gcttttttat ggccggttat cgcctcgaaa attgatcaaa catacgtatt
      121 atcttgcttt aattaattac actaatgctt cttcccttcg ttttagcgcc ccgccgcagt
      181 atcatgatat cgataaccat aataaatgtg tggtaaatgg cgcatcgatc gcattattga
      241 ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac ggaataacta taaatgactg
      301 gagataacac cctcatccat tctcacggca ttaaccgtcg tgatttcatg aagctttgtg
      361 cagcattagc cgccaccatg gggttaagta gcatactaga gtcacacagg aaagtactag
      421 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      481 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      541 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      601 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      661 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      721 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      781 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      841 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      901 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      961 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1021 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1081 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     1141 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1201 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1261 gcctttctgc gtttata
//