BBa_R0065 1 cI+luxR Promoter (lambda cI and luxR regulated -- hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z Released HQ 2013 cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. </P> <P>&nbsp;</P> <table width="75%" border="1"> <tr> <td><strong>LuxI</strong></td> <td><strong>cI</strong></td> <td><strong>activity of promoter</strong></td> </tr> <tr> <td>+</td> <td>+</td> <td>zero</td> </tr> <tr> <td>+</td> <td>-</td> <td>maximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky (no quantitative information)</td> </tr> </table> <P>&nbsp;</P> false false _1_ 0 24 7 In stock false <P> <P>This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation1986780 1 OR1 cI range1986780 1 81 97 annotation1986781 1 -10 range1986781 1 94 97 annotation1986779 1 -35 range1986779 1 71 76 annotation1986774 1 BBa_R0065 range1986774 1 1 97 annotation1986775 1 Lux Box range1986775 1 6 25 annotation1986778 1 lux p(R) start range1986778 1 58 58 annotation1986776 1 -10 range1986776 1 47 52 annotation1986777 1 OR2 cI range1986777 1 57 73 BBa_K193602 1 placIQ RBS placIQ RBS melA on pSB4A5 2009-10-13T11:00:00Z 2015-05-08T01:11:16Z We used I14032, B0030 and K193600. This composite part contains pLacIQ(BBa_I14032) and RBS(B0030) and melA on low copy vector(PSB4A5). false true _288_ 0 5245 9 It's complicated true We used biobrick standard assembly. false Kazuaki Amikura component2072762 1 BBa_K193600 component2072758 1 BBa_I14032 component2072760 1 BBa_B0030 annotation2072758 1 BBa_I14032 range2072758 1 1 37 annotation2072762 1 BBa_K193600 range2072762 1 67 1896 annotation2072760 1 BBa_B0030 range2072760 1 46 60 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_K584007 1 BBa_K584007 Lambda cI and LuxR regulated hybrid promotor + RBS + MelA 2011-08-15T11:00:00Z 2015-05-08T01:12:47Z / More information will be added later. true false _755_ 0 9879 9 Discontinued false / false Sarah Veugelen component2125269 1 BBa_K193602 component2125253 1 BBa_R0065 annotation2125253 1 BBa_R0065 range2125253 1 1 97 annotation2125269 1 BBa_K193602 range2125269 1 106 2001 BBa_K193600 1 BBa_K193600 melA gene 2009-10-13T11:00:00Z 2015-05-08T01:11:16Z The gene sequence was obtained from GENEART. melA is coding sequence of tyrosinase. Tyrosinase is enzyme which converts from tyrosine to dopaquinone. Dopaquinone is intermediate product of melanin biosynthesis pathway. false false _288_ 0 5245 9 Not in stock false We added restriction enzyme sites (Prefix-Suffix) to the upstream and down stream of the ORF of melA. false Kazuaki Amikura BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028344 1 -10 range1028344 1 26 31 annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028343 1 -35 range1028343 1 3 8 BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_B0030_sequence 1 attaaagaggagaaa BBa_K584007_sequence 1 taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa BBa_R0065_sequence 1 taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata BBa_K193602_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa BBa_K193600_sequence 1 atggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z