LOCUS       BBa_K584009             3467 bp    DNA     linear       16-Dec-2024
DEFINITION  Lac-Lux hybrid promotor + CrtEBI
ACCESSION   BBa_K584009
VERSION     BBa_K584009.1  
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /label=-35
     misc_feature    34..39
                     /label=-10
     misc_feature    16..33
                     /label=lux box
     misc_feature    40..74
                     /label=lacI binding site
     promoter        1..74
                     /label=BBa_K091100
     RBS             83..86
                     /label=BBa_K118012
     CDS             93..1004
                     /label=BBa_I742151
     misc_feature    1013..1015
                     /label=SacI
     RBS             1016..1022
                     /label=rbs
     RBS             1013..1022
                     /label=BBa_J15001
     CDS             1029..1958
                     /label=crtB coding sequence
     CDS             1029..1961
                     /label=BBa_K118002
     misc_feature    1970..1972
                     /label=SacI
     RBS             1973..1979
                     /label=rbs
     RBS             1970..1979
                     /label=BBa_J15001
     misc_feature    2463..2468
                     /label=PstI site removed
     misc_feature    2874..2879
                     /label=PstI site removed
     CDS             1986..3464
                     /label=crtI coding sequence
     CDS             1986..3467
                     /label=BBa_K118003
     misc_feature    83..3467
                     /label=BBa_K274100
ORIGIN
        1 accccaggct tgacacctgt aggatcgtac aggtataatt gtgtggaatt gtgagcggat
       61 aacaatttca cacatactag aggaggtact agatgacggt ctgcgcaaaa aaacacgttc
      121 atctcactcg cgatgctgcg gagcagttac tggctgatat tgatcgacgc cttgatcagt
      181 tattgcccgt ggagggagaa cgggatgttg tgggtgccgc gatgcgtgaa ggtgcgctgg
      241 caccgggaaa acgtattcgc cccatgttgc tgttgctgac cgcccgcgat ctgggttgcg
      301 ctgtcagcca tgacggatta ctggatttgg cctgtgcggt ggaaatggtc cacgcggctt
      361 cgctgatcct tgacgatatg ccctgcatgg acgatgcgaa gctgcggcgc ggacgcccta
      421 ccattcattc tcattacgga gagcatgtgg caatactggc ggcggttgcc ttgctgagta
      481 aagcctttgg cgtaattgcc gatgcagatg gcctcacgcc gctggcaaaa aatcgggcgg
      541 tttctgaact gtcaaacgcc atcggcatgc aaggattggt tcagggtcag ttcaaggatc
      601 tgtctgaagg ggataagccg cgcagcgctg aagctatttt gatgacgaat cactttaaaa
      661 ccagcacgct gttttgtgcc tccatgcaga tggcctcgat tgttgcgaat gcctccagcg
      721 aagcgcgtga ttgcctgcat cgtttttcac ttgatcttgg tcaggcattt caactgctgg
      781 acgatttgac cgatggcatg accgacaccg gtaaggatag caatcaggac gccggtaaat
      841 cgacgctggt caatctgtta ggcccgaggg cggttgaaga acgtctgaga caacatcttc
      901 agcttgccag tgagcatctc tctgcggcct gccaacacgg gcacgccact caacatttta
      961 ttcaggcctg gtttgacaaa aaactcgctg ccgtcagtta ataatactag agctcaagga
     1021 ggtactagat gaataatccg tcgttactca atcatgcggt cgaaacgatg gcagttggct
     1081 cgaaaagttt tgcgacagcc tcaaagttat ttgatgcaaa aacccggcgc agcgtactga
     1141 tgctctacgc ctggtgccgc cattgtgacg atgttattga cgatcagacg ctgggctttc
     1201 aggcccggca gcctgcctta caaacgcccg aacaacgtct gatgcaactt gagatgaaaa
     1261 cgcgccaggc ctatgcagga tcgcagatgc acgaaccggc gtttgcggct tttcaggaag
     1321 tggctatggc tcatgatatc gccccggctt acgcgtttga tcatctggaa ggcttcgcca
     1381 tggatgtacg cgaagcgcaa tacagccaac tggatgatac gctgcgctat tgctatcacg
     1441 ttgcaggcgt tgtcggcttg atgatggcgc aaatcatggg cgtgcgggat aacgccacgc
     1501 tggaccgcgc ctgtgacctt gggctggcat ttcagttgac caatattgct cgcgatattg
     1561 tggacgatgc gcatgcgggc cgctgttatc tgccggcaag ctggctggag catgaaggtc
     1621 tgaacaaaga gaattatgcg gcacctgaaa accgtcaggc gctgagccgt atcgcccgtc
     1681 gtttggtgca ggaagcagaa ccttactatt tgtctgccac agccggcctg gcagggttgc
     1741 ccctgcgttc cgcctgggca atcgctacgg cgaagcaggt ttaccggaaa ataggtgtca
     1801 aagttgaaca ggccggtcag caagcctggg atcagcggca gtcaacgacc acgcccgaaa
     1861 aattaacgct gctgctggcc gcctctggtc aggcccttac ttcccggatg cgggctcatc
     1921 ctccccgccc tgcgcatctc tggcagcgcc cgctctaata atactagagc tcaaggaggt
     1981 actagatgaa accaactacg gtaattggtg caggcttcgg tggcctggca ctggcaattc
     2041 gtctacaagc tgcggggatc cccgtcttac tgcttgaaca acgtgataaa cccggcggtc
     2101 gggcttatgt ctacgaggat caggggttta cctttgatgc aggcccgacg gttatcaccg
     2161 atcccagtgc cattgaagaa ctgtttgcac tggcaggaaa acagttaaaa gagtatgtcg
     2221 aactgctgcc ggttacgccg ttttaccgcc tgtgttggga gtcagggaag gtctttaatt
     2281 acgataacga tcaaacccgg ctcgaagcgc agattcagca gtttaatccc cgcgatgtcg
     2341 aaggttatcg tcagtttctg gactattcac gcgcggtgtt taaagaaggc tatctaaagc
     2401 tcggtactgt ccctttttta tcgttcagag acatgcttcg cgccgcacct caactggcga
     2461 aactgcaagc atggagaagc gtttacagta aggttgccag ttacatcgaa gatgaacatc
     2521 tgcgccaggc gttttctttc cactcgctgt tggtgggcgg caatcccttc gccacctcat
     2581 ccatttatac gttgatacac gcgctggagc gtgagtgggg cgtctggttt ccgcgtggcg
     2641 gcaccggcgc attagttcag gggatgataa agctgtttca ggatctgggt ggcgaagtcg
     2701 tgttaaacgc cagagtcagc catatggaaa cgacaggaaa caagattgaa gccgtgcatt
     2761 tagaggacgg tcgcaggttc ctgacgcaag ccgtcgcgtc aaatgcagat gtggttcata
     2821 cctatcgcga cctgttaagc cagcaccctg ccgcggttaa gcagtccaac aaactgcaaa
     2881 ctaagcgcat gagtaactct ctgtttgtgc tctattttgg tttgaatcac catcatgatc
     2941 agctcgcgca tcacacggtt tgtttcggcc cgcgttaccg cgagctgatt gacgaaattt
     3001 ttaatcatga tggcctcgca gaggacttct cactttatct gcacgcgccc tgtgtcacgg
     3061 attcgtcact ggcgcctgaa ggttgcggca gttactatgt gttggcgccg gtgccgcatt
     3121 taggcaccgc gaacctcgac tggacggttg aggggccaaa actacgcgac cgtatttttg
     3181 cgtaccttga gcagcattac atgcctggct tacggagtca gctggtcacg caccggatgt
     3241 ttacgccgtt tgattttcgc gaccagctta atgcctatca tggctcagcc ttttctgtgg
     3301 agcccgttct tacccagagc gcctggtttc ggccgcataa ccgcgataaa accattacta
     3361 atctctacct ggtcggcgca ggcacgcatc ccggcgcagg cattcctggc gtcatcggct
     3421 cggcaaaagc gacagcaggt ttgatgctgg aggatctgat ataataa
//