LOCUS BBa_K584010 4294 bp DNA linear 14-Nov-2024 DEFINITION Lac-Lux hybrid promotor + CrtEBI + CI repressor ACCESSION BBa_K584010 VERSION BBa_K584010.1 FEATURES Location/Qualifiers misc_feature 10..15 /label=-35 misc_feature 34..39 /label=-10 misc_feature 16..33 /label=lux box misc_feature 40..74 /label=lacI binding site promoter 1..74 /label=BBa_K091100 RBS 83..86 /label=BBa_K118012 CDS 93..1004 /label=BBa_I742151 misc_feature 1013..1015 /label=SacI RBS 1016..1022 /label=rbs RBS 1013..1022 /label=BBa_J15001 CDS 1029..1958 /label=crtB coding sequence CDS 1029..1961 /label=BBa_K118002 misc_feature 1970..1972 /label=SacI RBS 1973..1979 /label=rbs RBS 1970..1979 /label=BBa_J15001 misc_feature 2463..2468 /label=PstI site removed misc_feature 2874..2879 /label=PstI site removed CDS 1986..3464 /label=crtI coding sequence CDS 1986..3467 /label=BBa_K118003 misc_feature 83..3467 /label=BBa_K274100 CDS 3500..4207 /label=cI lambda misc_feature 4208..4240 /label=LVA misc_feature 4247..4271 /label=Help:Barcodes CDS 3497..4246 /label=BBa_C0051 RBS 3476..3490 /label=RBS-1\Strong RBS 3483..3487 /label=RBS misc_feature 3476..3490 /label=BBa_B0030 RBS 3476..3490 /label=BBa_B0030 CDS 3500..4207 /label=cI lambda misc_feature 4208..4240 /label=LVA misc_feature 4247..4271 /label=Help:Barcodes CDS 3497..4271 /label=BBa_C0051 RBS 4280..4294 /label=RBS-1\Strong RBS 4287..4291 /label=RBS misc_feature 4280..4294 /label=BBa_B0030 RBS 4280..4294 /label=BBa_B0030 misc_feature 3476..4294 /label=BBa_K081013 ORIGIN 1 accccaggct tgacacctgt aggatcgtac aggtataatt gtgtggaatt gtgagcggat 61 aacaatttca cacatactag aggaggtact agatgacggt ctgcgcaaaa aaacacgttc 121 atctcactcg cgatgctgcg gagcagttac tggctgatat tgatcgacgc cttgatcagt 181 tattgcccgt ggagggagaa cgggatgttg tgggtgccgc gatgcgtgaa ggtgcgctgg 241 caccgggaaa acgtattcgc cccatgttgc tgttgctgac cgcccgcgat ctgggttgcg 301 ctgtcagcca tgacggatta ctggatttgg cctgtgcggt ggaaatggtc cacgcggctt 361 cgctgatcct tgacgatatg ccctgcatgg acgatgcgaa gctgcggcgc ggacgcccta 421 ccattcattc tcattacgga gagcatgtgg caatactggc ggcggttgcc ttgctgagta 481 aagcctttgg cgtaattgcc gatgcagatg gcctcacgcc gctggcaaaa aatcgggcgg 541 tttctgaact gtcaaacgcc atcggcatgc aaggattggt tcagggtcag ttcaaggatc 601 tgtctgaagg ggataagccg cgcagcgctg aagctatttt gatgacgaat cactttaaaa 661 ccagcacgct gttttgtgcc tccatgcaga tggcctcgat tgttgcgaat gcctccagcg 721 aagcgcgtga ttgcctgcat cgtttttcac ttgatcttgg tcaggcattt caactgctgg 781 acgatttgac cgatggcatg accgacaccg gtaaggatag caatcaggac gccggtaaat 841 cgacgctggt caatctgtta ggcccgaggg cggttgaaga acgtctgaga caacatcttc 901 agcttgccag tgagcatctc tctgcggcct gccaacacgg gcacgccact caacatttta 961 ttcaggcctg gtttgacaaa aaactcgctg ccgtcagtta ataatactag agctcaagga 1021 ggtactagat gaataatccg tcgttactca atcatgcggt cgaaacgatg gcagttggct 1081 cgaaaagttt tgcgacagcc tcaaagttat ttgatgcaaa aacccggcgc agcgtactga 1141 tgctctacgc ctggtgccgc cattgtgacg atgttattga cgatcagacg ctgggctttc 1201 aggcccggca gcctgcctta caaacgcccg aacaacgtct gatgcaactt gagatgaaaa 1261 cgcgccaggc ctatgcagga tcgcagatgc acgaaccggc gtttgcggct tttcaggaag 1321 tggctatggc tcatgatatc gccccggctt acgcgtttga tcatctggaa ggcttcgcca 1381 tggatgtacg cgaagcgcaa tacagccaac tggatgatac gctgcgctat tgctatcacg 1441 ttgcaggcgt tgtcggcttg atgatggcgc aaatcatggg cgtgcgggat aacgccacgc 1501 tggaccgcgc ctgtgacctt gggctggcat ttcagttgac caatattgct cgcgatattg 1561 tggacgatgc gcatgcgggc cgctgttatc tgccggcaag ctggctggag catgaaggtc 1621 tgaacaaaga gaattatgcg gcacctgaaa accgtcaggc gctgagccgt atcgcccgtc 1681 gtttggtgca ggaagcagaa ccttactatt tgtctgccac agccggcctg gcagggttgc 1741 ccctgcgttc cgcctgggca atcgctacgg cgaagcaggt ttaccggaaa ataggtgtca 1801 aagttgaaca ggccggtcag caagcctggg atcagcggca gtcaacgacc acgcccgaaa 1861 aattaacgct gctgctggcc gcctctggtc aggcccttac ttcccggatg cgggctcatc 1921 ctccccgccc tgcgcatctc tggcagcgcc cgctctaata atactagagc tcaaggaggt 1981 actagatgaa accaactacg gtaattggtg caggcttcgg tggcctggca ctggcaattc 2041 gtctacaagc tgcggggatc cccgtcttac tgcttgaaca acgtgataaa cccggcggtc 2101 gggcttatgt ctacgaggat caggggttta cctttgatgc aggcccgacg gttatcaccg 2161 atcccagtgc cattgaagaa ctgtttgcac tggcaggaaa acagttaaaa gagtatgtcg 2221 aactgctgcc ggttacgccg ttttaccgcc tgtgttggga gtcagggaag gtctttaatt 2281 acgataacga tcaaacccgg ctcgaagcgc agattcagca gtttaatccc cgcgatgtcg 2341 aaggttatcg tcagtttctg gactattcac gcgcggtgtt taaagaaggc tatctaaagc 2401 tcggtactgt ccctttttta tcgttcagag acatgcttcg cgccgcacct caactggcga 2461 aactgcaagc atggagaagc gtttacagta aggttgccag ttacatcgaa gatgaacatc 2521 tgcgccaggc gttttctttc cactcgctgt tggtgggcgg caatcccttc gccacctcat 2581 ccatttatac gttgatacac gcgctggagc gtgagtgggg cgtctggttt ccgcgtggcg 2641 gcaccggcgc attagttcag gggatgataa agctgtttca ggatctgggt ggcgaagtcg 2701 tgttaaacgc cagagtcagc catatggaaa cgacaggaaa caagattgaa gccgtgcatt 2761 tagaggacgg tcgcaggttc ctgacgcaag ccgtcgcgtc aaatgcagat gtggttcata 2821 cctatcgcga cctgttaagc cagcaccctg ccgcggttaa gcagtccaac aaactgcaaa 2881 ctaagcgcat gagtaactct ctgtttgtgc tctattttgg tttgaatcac catcatgatc 2941 agctcgcgca tcacacggtt tgtttcggcc cgcgttaccg cgagctgatt gacgaaattt 3001 ttaatcatga tggcctcgca gaggacttct cactttatct gcacgcgccc tgtgtcacgg 3061 attcgtcact ggcgcctgaa ggttgcggca gttactatgt gttggcgccg gtgccgcatt 3121 taggcaccgc gaacctcgac tggacggttg aggggccaaa actacgcgac cgtatttttg 3181 cgtaccttga gcagcattac atgcctggct tacggagtca gctggtcacg caccggatgt 3241 ttacgccgtt tgattttcgc gaccagctta atgcctatca tggctcagcc ttttctgtgg 3301 agcccgttct tacccagagc gcctggtttc ggccgcataa ccgcgataaa accattacta 3361 atctctacct ggtcggcgca ggcacgcatc ccggcgcagg cattcctggc gtcatcggct 3421 cggcaaaagc gacagcaggt ttgatgctgg aggatctgat ataataatac tagagattaa 3481 agaggagaaa tactagatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga 3541 cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga 3601 atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat 3661 caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga 3721 agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca 3781 gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt 3841 ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa 3901 aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac 3961 aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt 4021 tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact 4081 gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc 4141 atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac 4201 gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta 4261 gtgtagatcg ctactagaga ttaaagagga gaaa //