##gff-version 3
##sequence-region BBa_K584010 1 4294
BBa_K584010	.	engineered_region	3476	4294	.	+	0	ID=BBa_K081013;Name=BBa_K081013
BBa_K584010	.	ribosome_entry_site	3476	3490	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081013
BBa_K584010	.	engineered_region	3476	3490	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K584010	.	ribosome_entry_site	3476	3490	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K584010	.	ribosome_entry_site	3483	3487	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K584010	.	CDS	3497	4271	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K081013
BBa_K584010	.	tag	4208	4240	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K584010	.	CDS	3500	4207	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K584010	.	engineered_tag	4247	4271	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K584010	.	ribosome_entry_site	4280	4294	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081013
BBa_K584010	.	engineered_region	4280	4294	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K584010	.	ribosome_entry_site	4280	4294	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K584010	.	ribosome_entry_site	4287	4291	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K584010	.	promoter	1	74	.	+	0	ID=BBa_K091100;Name=BBa_K091100
BBa_K584010	.	non_covalent_binding_site	16	33	.	+	0	ID=annotation1963335;Name=lux box;Parent=BBa_K091100
BBa_K584010	.	sequence_feature	34	39	.	+	0	ID=annotation1963334;Name=-10;Parent=BBa_K091100
BBa_K584010	.	sequence_feature	10	15	.	+	0	ID=annotation1963333;Name=-35;Parent=BBa_K091100
BBa_K584010	.	non_covalent_binding_site	40	74	.	+	0	ID=annotation1963336;Name=lacI binding site;Parent=BBa_K091100
BBa_K584010	.	engineered_region	83	3467	.	+	0	ID=BBa_K274100;Name=BBa_K274100
BBa_K584010	.	CDS	1986	3467	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_K584010	.	CDS	1986	3464	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K584010	.	point_mutation	2874	2879	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K584010	.	point_mutation	2463	2468	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K584010	.	CDS	1029	1961	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_K584010	.	CDS	1029	1958	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K584010	.	ribosome_entry_site	83	86	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
BBa_K584010	.	CDS	93	1004	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_K584010	.	ribosome_entry_site	1970	1979	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K584010	.	sequence_feature	1970	1972	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K584010	.	ribosome_entry_site	1973	1979	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K584010	.	ribosome_entry_site	1013	1022	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K584010	.	sequence_feature	1013	1015	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K584010	.	ribosome_entry_site	1016	1022	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K584010	.	CDS	3497	4246	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K584010	.	tag	4208	4240	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K584010	.	CDS	3500	4207	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K584010	.	engineered_tag	4247	4271	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_K584010
accccaggcttgacacctgtaggatcgtacaggtataattgtgtggaattgtgagcggataacaatttcacacatacta
gaggaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgat
attgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgc
tggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacgg
attactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgat
gcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgc
tgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtc
aaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaa
gctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatg
cctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgac
cgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagg
gcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgcca
ctcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtacta
gatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaag
ttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatc
agacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcg
ccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgcc
ccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgc
tgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgct
ggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggc
cgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcagg
cgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggtt
gcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggt
cagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggccc
ttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaag
gaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcg
gggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacct
ttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaaga
gtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgat
caaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcac
gcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacc
tcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccag
gcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctgg
agcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatct
gggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagag
gacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagc
accctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggttt
gaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaattttt
aatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaag
gttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaa
actacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatg
tttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccaga
gcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgc
aggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataatactagaga
ttaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaa
agcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtca
ggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagtta
gcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttag
aagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggt
gatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatga
ccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccagg
tgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgttttta
caaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagt
ggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtag
atcgctactagagattaaagaggagaaa