BBa_R0065
1
cI+luxR
Promoter (lambda cI and luxR regulated -- hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
Released HQ 2013
cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. </P> <P> </P> <table width="75%" border="1"> <tr> <td><strong>LuxI</strong></td> <td><strong>cI</strong></td> <td><strong>activity of promoter</strong></td> </tr> <tr> <td>+</td> <td>+</td> <td>zero</td> </tr> <tr> <td>+</td> <td>-</td> <td>maximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky (no quantitative information)</td> </tr> </table> <P> </P>
false
false
_1_
0
24
7
In stock
false
<P> <P>This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1986781
1
-10
range1986781
1
94
97
annotation1986779
1
-35
range1986779
1
71
76
annotation1986778
1
lux p(R) start
range1986778
1
58
58
annotation1986776
1
-10
range1986776
1
47
52
annotation1986774
1
BBa_R0065
range1986774
1
1
97
annotation1986780
1
OR1 cI
range1986780
1
81
97
annotation1986777
1
OR2 cI
range1986777
1
57
73
annotation1986775
1
Lux Box
range1986775
1
6
25
BBa_K584029
1
BBa_K584029
Ribolock + ceaB + Terminator
2011-09-18T11:00:00Z
2015-05-08T01:12:47Z
This is a basic biobrick we made for our project. We performed a PCR to get the CeaB part of the K131009 biobrick. The primer design for this was such that it already contained the ribolock resulting in ???Ribolock + ceaB??? as the final product. This is fused with the terminator B0015 to get ???Ribolock + ceaB + B0015???.
Therefore ???Ribolock + ceaB + B0015??? is a basic buildig block.
false
false
_755_
0
9860
9
It's complicated
true
false
Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
annotation2134150
1
Ribolock
range2134150
1
1
42
annotation2134151
1
ceaB
range2134151
1
49
1794
annotation2134152
1
Terminator (B0015)
range2134152
1
1803
1932
BBa_K584015
1
BBa_K584015
Lambda cI and LuxR regulated hybrid promoter + Ribolock-CeaB + Terminator
2011-08-15T11:00:00Z
2015-05-08T01:12:47Z
/
More information will be added soon.
false
false
_755_
0
9879
9
Not in stock
false
/
false
Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
component2134192
1
BBa_K584029
component2134181
1
BBa_R0065
annotation2134192
1
BBa_K584029
range2134192
1
106
2037
annotation2134181
1
BBa_R0065
range2134181
1
1
97
BBa_K584029_sequence
1
aactagaatcacctcttgcttttgggtaagacgaagaggagatactagatgagcggtggcgatggacgcggccataacacgggcgcgcatagcacaagtggtaacattaatggtggcccgaccgggcttggtgtaggtggtggtgcttctgatggctccggatggagttcggaaaataacccgtggggtggtggttccggtagcggcattcactggggtggtggttccggtcatggtaatggcggggggaatggtaattccggtggtggttcgggaacaggcggtaatctgtcagcagtagctgcgccagtggcatttggttttccggcactttccactccaggagctggcggtctggcggtcagtatttcagcgggagcattatcggcagctattgctgatattatggctgccctgaaaggaccgtttaaatttggtctttggggggtggctttatatggtgtattgccatcacaaatagcgaaagatgaccccaatatgatgtcaaagattgtgacgtcattacccgcagatgatattactgaatcacctgtcagttcattacctctcgataaggcaacagtaaacgtaaatgttcgtgttgttgatgatgtaaaagacgaacgacagaatatttcggttgtttcaggtgttccgatgagtgttccggtggttgatgcaaaacctaccgaacgtccaggtgtttttacggcatcaattccaggtgcacctgttctgaatatttcagttaataacagtacgccagaagtacagacattaagcccaggtgttacaaataatactgataaggatgttcgcccggcaggatttactcagggtggtaataccagggatgcagttattcgattcccgaaggacagcggtcataatgccgtatatgtttcagtgagtgatgttcttagtcctgaccaggtaaaacaacgtcaggatgaagaaaatcgccgtcagcaggaatgggatgctacgcatccggttgaagcggctgagcgaaattatgaacgcgcgcgtgcagagctgaatcaggcaaatgaagatgttgccagaaatcaggagcgacaggctaaagctgttcaggtttataattcgcgtaaaagcgaacttgatgcagcgaataaaactcttgctgatgcaatagctgaaataaaacaatttaatcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccggacttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaaactttgacgatttccggaagaaattctgggaagaagtgtcaaaagatcccgatcttagtaagcaatttaaaggcagtaataagacgaacattcaaaagggaaaagcaccttttgcaaggaagaaagaccaagtaggtggtagggaacgctttgaattacatcatgataaaccaatcagtcaggatggtggtgtctatgatatgaataatatcagagtgaccacacctaagcgacatattgatattcatcggggtaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
BBa_K584015_sequence
1
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaactagaatcacctcttgcttttgggtaagacgaagaggagatactagatgagcggtggcgatggacgcggccataacacgggcgcgcatagcacaagtggtaacattaatggtggcccgaccgggcttggtgtaggtggtggtgcttctgatggctccggatggagttcggaaaataacccgtggggtggtggttccggtagcggcattcactggggtggtggttccggtcatggtaatggcggggggaatggtaattccggtggtggttcgggaacaggcggtaatctgtcagcagtagctgcgccagtggcatttggttttccggcactttccactccaggagctggcggtctggcggtcagtatttcagcgggagcattatcggcagctattgctgatattatggctgccctgaaaggaccgtttaaatttggtctttggggggtggctttatatggtgtattgccatcacaaatagcgaaagatgaccccaatatgatgtcaaagattgtgacgtcattacccgcagatgatattactgaatcacctgtcagttcattacctctcgataaggcaacagtaaacgtaaatgttcgtgttgttgatgatgtaaaagacgaacgacagaatatttcggttgtttcaggtgttccgatgagtgttccggtggttgatgcaaaacctaccgaacgtccaggtgtttttacggcatcaattccaggtgcacctgttctgaatatttcagttaataacagtacgccagaagtacagacattaagcccaggtgttacaaataatactgataaggatgttcgcccggcaggatttactcagggtggtaataccagggatgcagttattcgattcccgaaggacagcggtcataatgccgtatatgtttcagtgagtgatgttcttagtcctgaccaggtaaaacaacgtcaggatgaagaaaatcgccgtcagcaggaatgggatgctacgcatccggttgaagcggctgagcgaaattatgaacgcgcgcgtgcagagctgaatcaggcaaatgaagatgttgccagaaatcaggagcgacaggctaaagctgttcaggtttataattcgcgtaaaagcgaacttgatgcagcgaataaaactcttgctgatgcaatagctgaaataaaacaatttaatcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccggacttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaaactttgacgatttccggaagaaattctgggaagaagtgtcaaaagatcccgatcttagtaagcaatttaaaggcagtaataagacgaacattcaaaagggaaaagcaccttttgcaaggaagaaagaccaagtaggtggtagggaacgctttgaattacatcatgataaaccaatcagtcaggatggtggtgtctatgatatgaataatatcagagtgaccacacctaagcgacatattgatattcatcggggtaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
BBa_R0065_sequence
1
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z