BBa_K584018
1
BBa_K584018
OmpA+Linker+point_Mutant_HPLC6(AFP)
2011-09-15T11:00:00Z
2015-05-08T01:12:47Z
Source - Pseudopleuronectes americanus (winter flounder)
gene="HPLC6"
This is basic part which has a sequence containing the OmpA Transmembrane Domain, linked to the point mutant HPLC6 (Anti-freeze protein) gene.
This part cna be traslated to produce the Antifreeze protein on the outermembrane of the E.Coli. The function of this protein is to raise the Freezing Point.
true
false
_755_
0
9860
9
Discontinued
false
A point mutation was necassary as the natural gene had the Pst1 restriction enzyme sequence in it.
false
Bakul Jitendra Vinchhi
annotation2129391
1
OmpA
range2129391
1
1
433
annotation2129393
1
HPLC6(AFP)
range2129393
1
459
707
annotation2129392
1
Linker
range2129392
1
434
458
BBa_K584018_sequence
1
atgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagcatggctctctcacttttcactgtcggacaattgattttcttattttggacaatgagaatcactgaagccagccccgaccccgcagccaaagccgccccagcagcagttgccgcccctgccgcagccgccccagacaccgcctctgacgccgccgccgcagccgcccttaccgccgccaacgccaaagccgctgccgaactcactgccgccaacgccgccgccgccgcagcagccaccgccagaggttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z