BBa_K584018 1 BBa_K584018 OmpA+Linker+point_Mutant_HPLC6(AFP) 2011-09-15T11:00:00Z 2015-05-08T01:12:47Z Source - Pseudopleuronectes americanus (winter flounder) gene="HPLC6" This is basic part which has a sequence containing the OmpA Transmembrane Domain, linked to the point mutant HPLC6 (Anti-freeze protein) gene. This part cna be traslated to produce the Antifreeze protein on the outermembrane of the E.Coli. The function of this protein is to raise the Freezing Point. true false _755_ 0 9860 9 Discontinued false A point mutation was necassary as the natural gene had the Pst1 restriction enzyme sequence in it. false Bakul Jitendra Vinchhi annotation2129391 1 OmpA range2129391 1 1 433 annotation2129393 1 HPLC6(AFP) range2129393 1 459 707 annotation2129392 1 Linker range2129392 1 434 458 BBa_K584018_sequence 1 atgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagcatggctctctcacttttcactgtcggacaattgattttcttattttggacaatgagaatcactgaagccagccccgaccccgcagccaaagccgccccagcagcagttgccgcccctgccgcagccgccccagacaccgcctctgacgccgccgccgcagccgcccttaccgccgccaacgccaaagccgctgccgaactcactgccgccaacgccgccgccgccgcagcagccaccgccagaggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z