LOCUS       BBa_K584020              871 bp    DNA     linear       28-Jun-2024
DEFINITION  AFP extracellular protein generator
ACCESSION   BBa_K584020
VERSION     BBa_K584020.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..452
                     /label=OmpA
     misc_feature    453..477
                     /label=Flexible_Linker
     CDS             478..726
                     /label=HPLC6(AFP)
     misc_feature    633..633
                     /label=HPLC6_Point_Mutant
     misc_feature    19..734
                     /label=BBa_K584019
     stem_loop       754..797
                     /label=stem_loop
     misc_feature    743..822
                     /label=BBa_B0010
     terminator      743..822
                     /label=BBa_B0010
     stem_loop       838..857
                     /label=T7 TE
     misc_feature    864..864
                     /label=stop
     polyA_site      858..871
                     /label=polya
     misc_feature    831..871
                     /label=BBa_B0012
     terminator      831..871
                     /label=BBa_B0012
     terminator      743..871
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaaagctact aaactggtac tgggcgcggt aatcctgggt
       61 tctactctgc tggcaggttg ctccagcaac gctaaaatcg atcagggaat taacccgtat
      121 gttggctttg aaatgggtta cgactggtta ggtcgtatgc cgtacaaagg cagcgttgaa
      181 aacggtgcat acaaagctca gggcgttcaa ctgaccgcta aactgggtta cccaatcact
      241 gacgacctgg acatctacac tcgtctgggt ggcatggtat ggcgtgcaga cactaaatcc
      301 aacgtttatg gtaaaaacca cgacaccggc gtttctccgg tcttcgctgg cggtgttgag
      361 tacgcgatca ctcctgaaat cgctacccgt ctggaatacc agtggaccaa caacatcggt
      421 gacgcacaca ccatcggcac tcgtccggac aacggcggag gttctggagg agggagcatg
      481 gctctctcac ttttcactgt cggacaattg attttcttat tttggacaat gagaatcact
      541 gaagccagcc ccgaccccgc agccaaagcc gccccagcag cagttgccgc ccctgccgca
      601 gccgccccag acaccgcctc tgacgccgcc gccgcagccg cccttaccgc cgccaacgcc
      661 aaagccgctg ccgaactcac tgccgccaac gccgccgccg ccgcagcagc caccgccaga
      721 ggttaatact agagtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      841 tcaccttcgg gtgggccttt ctgcgtttat a
//