LOCUS BBa_K584024 3758 bp DNA linear 22-Dec-2024 DEFINITION Ice Nucleating Protein Generator (RBS + InaZ [K584021] + Terminator) ACCESSION BBa_K584024 VERSION BBa_K584024.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..3621 /label=InaZ CDS 19..3621 /label=BBa_K584021 stem_loop 3641..3684 /label=stem_loop misc_feature 3630..3709 /label=BBa_B0010 terminator 3630..3709 /label=BBa_B0010 stem_loop 3725..3744 /label=T7 TE misc_feature 3751..3751 /label=stop polyA_site 3745..3758 /label=polya misc_feature 3718..3758 /label=BBa_B0012 terminator 3718..3758 /label=BBa_B0012 terminator 3630..3758 /label=BBa_B0015 ORIGIN 1 aaagaggaga aatactagat gaatctcgac aaggcgttgg tgctgcgtac ctgtgcaaat 61 aacatggccg atcactgcgg ccttatatgg cccgcgtccg gcacggtgga atccagatac 121 tggcagtcaa ccaggcggca tgagaatggt ctggtcggtt tactgtgggg cgctggaacc 181 agcgcttttc taagcgtgca tgccgatgct cgatggattg tctgtgaagt tgccgttgca 241 gacatcatca gtctggaaga gccgggaatg gtcaagtttc cgcgggccga ggtggttcat 301 gtcggcgaca ggatcagcgc gtcacacttc atttcggcac gtcaggccga ccctgcgtca 361 acatcaacat caacgttaac gccaatgccc actgccatac ccacgcccat gcctgccgta 421 gcaagtgtca cgttaccggt ggccgaacag gcccgtcatg aagtgttcga tgtcgcgtcg 481 gtcagcgcgg ctgccgcccc agtaaacacc ctgccggtga cgacgccgca gaatgtgcag 541 accgccactt acggcagcac gttgagtggc gacaatcaca gtcgtctgat tgccggttat 601 ggcagtaacg agaccgctgg caaccacagt gatctaattg ccggttatgg aagtacaggc 661 accgccggct ccgacagctg gctggtcgct ggctatggaa gcacccagac cgccggtggg 721 gacagcgcgc tgacagcggg ttacggcagc acccagaccg cccgcgaagg cagcaacctg 781 acggcagggt acggcagcac cggcacggca ggctcggaca gttcgctgat cgccggttac 841 ggcagtactc agacttcggg cggggacagc tcactcacag cgggttacgg cagcacgcaa 901 acggctcagg aaggcagcaa tctcaccgct gggtatggca gcaccggcac ggcaggctcg 961 gacagctcct tgatcgccgg ttatggcagt acacaaacct cgggaggcga cagttcgctg 1021 accgcgggct acggcagtac gcagacggcc caggagggca gcaatctgac ggcggggtac 1081 ggcagcacgg gtacagcagg tgtcgacagc tctctgatcg cgggatacgg cagcacgcag 1141 acctcgggaa gtgacagcgc cctgaccgca ggctatggca gcacgcaaac ggcccaggaa 1201 ggcagcaatc tcactgctgg gtatggcagc accggcacgg caggttccga cagctcgctg 1261 atcgccggtt acggcagcac gcaaacctcg ggcagtgaca gctcgctcac ggcggggtac 1321 ggcagtacgc agacggctca ggaaggcagc attctgacgg cggggtacgg cagcacgggt 1381 acagcaggtg tcgacagttc gttgatcgcc ggatatggca gcacgcagac ctcgggaagt 1441 gacagtgcgc tgacagcggg ttacggcagc acgcaaacgg cccaggaagg cagcaacctg 1501 acggcgggct acggcagcac tggcacggca ggtgccgaca gttcgttgat cgccggatat 1561 ggcagcacgc agacgtcagg cagcgaaagt tcgcttaccg caggctatgg cagtacccag 1621 actgcccgtg agggcagcac cctgacggcc ggatatggca gtaccggaac agctggcgct 1681 gacagctcgc tgatcgccgg ttacggcagc acgcaaacct cgggcagtga aagctcgctc 1741 acggcaggtt atggcagtac ccagaccgca cagcagggca gcgtactcac atcaggctat 1801 ggcagtacgc aaacggccgg ggctgccagt aacctcacca ccggttacgg aagtacaggt 1861 accgcaggtc acgagagttt catcattgcg ggttatggaa gtacacagac agcgggccac 1921 aaaagtatcc tgaccgctgg ttatggcagt actcagacgg ccagggacgg tagcgacctg 1981 attgcgggct atggcagtac gggaaccgca ggctcgggca gttcgctgat cgcaggttat 2041 ggcagcaccc agaccgcgag ttacagaagc atgctgaccg ccggttatgg cagtacccag 2101 accgccagag aacacagcga ccttgtcaca ggctatggca gcacttcaac ggcagggtca 2161 aacagttcgc tgatcgccgg ctatggaagc actcagacgg cgggcttcaa aagcatactg 2221 accgccggtt acggcagtac ccagacggca caggagcgca cgagcctggt cgcaggctac 2281 ggaagcacgt cgactgcggg ctattccagt tccttgatcg ccggctatgg cagcacgcag 2341 acggcaggct acgaaagcac gttgaccgcc ggttacggca gtacgcaaac cgctcaggaa 2401 aacagctcgc tcaccacagg ttacggaagt acttctactg cgggctattc cagctcgctc 2461 atcgcgggtt acggcagtac gcaaacggca ggctacgaga gcacgttgac cgccggttac 2521 ggtagtacgc aaaccgcgca ggagcgcagt gatctggtga caggttatgg aagtacctcc 2581 accgccggct atgcgagctc gctgattgcg ggttatggca gcacgcagac tgcgggttat 2641 gagagcacgt tgaccgccgg ttacggcagc acgcaaaccg cacaggaaaa cagctcgctc 2701 accaccgggt acggaagtac ctccacagcc ggctttgcca gctcgctgat ctccggttat 2761 ggcagtacgc agacagccgg ctataaaagt accctcacgg ccggttacgg cagtactcag 2821 accgcagagt atggaagctc actcactgcg ggctacggca gcactgcaac ggccgggcag 2881 gacagttcat tgatagccgg ctatggcagc tccctgacca gcggaatcag aagttttctg 2941 acggcaggct atggcagtac gctgatcgcc ggacttcgca gcgttttgat cgccggttat 3001 ggcagtagtc ttacatcggg cgttcgcagc acgttgactg cgggttatgg cagtaaccag 3061 attgcaagtt acggcagctc gttgattgca ggccatgaaa gcattcaggt cgccggaaat 3121 aaaagcatgc tgatcgccgg caagggcagc tcgcagacag caggttttcg cagcacgctg 3181 attgccggtg cgggcagtgt acaactggcg ggtgatcgca gccggttgat tgccggtgca 3241 gacagtaatc agaccgcggg tgaccgcagc aaactgctgg ccggtaataa cagttatctg 3301 actgccggcg atagaagcaa actgaccggc gggcatgact gcaccctgat ggcgggagac 3361 caaagcagat tgaccgctgg taagaacagt gtcttgacgg caggcgctcg tagcaaactg 3421 attggcagtg aaggctcgac gctctcggct ggagaagact ccatacttat tttcaggctc 3481 tgggacggga agaggtacag gcaactggtc gccagaacgg gtgagaacgg tgttgaggcc 3541 gacataccgt attacgtgaa cgaagatgac gatattgtcg ataaacccga cgaggacgat 3601 gactggatag aggtaaagta gtactagagc caggcatcaa ataaaacgaa aggctcagtc 3661 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3721 cactggctca ccttcgggtg ggcctttctg cgtttata //