LOCUS       BBa_K584026              914 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive_Promoter + AFP(Antifreeze extra-cellular Protein 
            Generator)
ACCESSION   BBa_K584026
VERSION     BBa_K584026.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..495
                     /label=OmpA
     misc_feature    496..520
                     /label=Flexible_Linker
     CDS             521..769
                     /label=HPLC6(AFP)
     misc_feature    676..676
                     /label=HPLC6_Point_Mutant
     misc_feature    62..777
                     /label=BBa_K584019
     stem_loop       797..840
                     /label=stem_loop
     misc_feature    786..865
                     /label=BBa_B0010
     terminator      786..865
                     /label=BBa_B0010
     stem_loop       881..900
                     /label=T7 TE
     misc_feature    907..907
                     /label=stop
     polyA_site      901..914
                     /label=polya
     misc_feature    874..914
                     /label=BBa_B0012
     terminator      874..914
                     /label=BBa_B0012
     terminator      786..914
                     /label=BBa_B0015
     misc_feature    44..914
                     /label=BBa_K584020
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaagct actaaactgg tactgggcgc ggtaatcctg ggttctactc tgctggcagg
      121 ttgctccagc aacgctaaaa tcgatcaggg aattaacccg tatgttggct ttgaaatggg
      181 ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc
      241 tcagggcgtt caactgaccg ctaaactggg ttacccaatc actgacgacc tggacatcta
      301 cactcgtctg ggtggcatgg tatggcgtgc agacactaaa tccaacgttt atggtaaaaa
      361 ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactcctga
      421 aatcgctacc cgtctggaat accagtggac caacaacatc ggtgacgcac acaccatcgg
      481 cactcgtccg gacaacggcg gaggttctgg aggagggagc atggctctct cacttttcac
      541 tgtcggacaa ttgattttct tattttggac aatgagaatc actgaagcca gccccgaccc
      601 cgcagccaaa gccgccccag cagcagttgc cgcccctgcc gcagccgccc cagacaccgc
      661 ctctgacgcc gccgccgcag ccgcccttac cgccgccaac gccaaagccg ctgccgaact
      721 cactgccgcc aacgccgccg ccgccgcagc agccaccgcc agaggttaat actagagtac
      781 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tata
//