LOCUS BBa_K584028 3840 bp DNA linear 22-Dec-2024 DEFINITION pLac-Lux hybrid promoter + INP(Ice Nucleating Protein Generator) ACCESSION BBa_K584028 VERSION BBa_K584028.1 FEATURES Location/Qualifiers misc_feature 10..15 /label=-35 misc_feature 34..39 /label=-10 misc_feature 16..33 /label=lux box misc_feature 40..74 /label=lacI binding site promoter 1..74 /label=BBa_K091100 misc_feature 87..90 /label=conserved RBS 83..94 /label=BBa_B0034 CDS 101..3703 /label=InaZ CDS 101..3703 /label=BBa_K584021 stem_loop 3723..3766 /label=stem_loop misc_feature 3712..3791 /label=BBa_B0010 terminator 3712..3791 /label=BBa_B0010 stem_loop 3807..3826 /label=T7 TE misc_feature 3833..3833 /label=stop polyA_site 3827..3840 /label=polya misc_feature 3800..3840 /label=BBa_B0012 terminator 3800..3840 /label=BBa_B0012 terminator 3712..3840 /label=BBa_B0015 misc_feature 83..3840 /label=BBa_K584024 ORIGIN 1 accccaggct tgacacctgt aggatcgtac aggtataatt gtgtggaatt gtgagcggat 61 aacaatttca cacatactag agaaagagga gaaatactag atgaatctcg acaaggcgtt 121 ggtgctgcgt acctgtgcaa ataacatggc cgatcactgc ggccttatat ggcccgcgtc 181 cggcacggtg gaatccagat actggcagtc aaccaggcgg catgagaatg gtctggtcgg 241 tttactgtgg ggcgctggaa ccagcgcttt tctaagcgtg catgccgatg ctcgatggat 301 tgtctgtgaa gttgccgttg cagacatcat cagtctggaa gagccgggaa tggtcaagtt 361 tccgcgggcc gaggtggttc atgtcggcga caggatcagc gcgtcacact tcatttcggc 421 acgtcaggcc gaccctgcgt caacatcaac atcaacgtta acgccaatgc ccactgccat 481 acccacgccc atgcctgccg tagcaagtgt cacgttaccg gtggccgaac aggcccgtca 541 tgaagtgttc gatgtcgcgt cggtcagcgc ggctgccgcc ccagtaaaca ccctgccggt 601 gacgacgccg cagaatgtgc agaccgccac ttacggcagc acgttgagtg gcgacaatca 661 cagtcgtctg attgccggtt atggcagtaa cgagaccgct ggcaaccaca gtgatctaat 721 tgccggttat ggaagtacag gcaccgccgg ctccgacagc tggctggtcg ctggctatgg 781 aagcacccag accgccggtg gggacagcgc gctgacagcg ggttacggca gcacccagac 841 cgcccgcgaa ggcagcaacc tgacggcagg gtacggcagc accggcacgg caggctcgga 901 cagttcgctg atcgccggtt acggcagtac tcagacttcg ggcggggaca gctcactcac 961 agcgggttac ggcagcacgc aaacggctca ggaaggcagc aatctcaccg ctgggtatgg 1021 cagcaccggc acggcaggct cggacagctc cttgatcgcc ggttatggca gtacacaaac 1081 ctcgggaggc gacagttcgc tgaccgcggg ctacggcagt acgcagacgg cccaggaggg 1141 cagcaatctg acggcggggt acggcagcac gggtacagca ggtgtcgaca gctctctgat 1201 cgcgggatac ggcagcacgc agacctcggg aagtgacagc gccctgaccg caggctatgg 1261 cagcacgcaa acggcccagg aaggcagcaa tctcactgct gggtatggca gcaccggcac 1321 ggcaggttcc gacagctcgc tgatcgccgg ttacggcagc acgcaaacct cgggcagtga 1381 cagctcgctc acggcggggt acggcagtac gcagacggct caggaaggca gcattctgac 1441 ggcggggtac ggcagcacgg gtacagcagg tgtcgacagt tcgttgatcg ccggatatgg 1501 cagcacgcag acctcgggaa gtgacagtgc gctgacagcg ggttacggca gcacgcaaac 1561 ggcccaggaa ggcagcaacc tgacggcggg ctacggcagc actggcacgg caggtgccga 1621 cagttcgttg atcgccggat atggcagcac gcagacgtca ggcagcgaaa gttcgcttac 1681 cgcaggctat ggcagtaccc agactgcccg tgagggcagc accctgacgg ccggatatgg 1741 cagtaccgga acagctggcg ctgacagctc gctgatcgcc ggttacggca gcacgcaaac 1801 ctcgggcagt gaaagctcgc tcacggcagg ttatggcagt acccagaccg cacagcaggg 1861 cagcgtactc acatcaggct atggcagtac gcaaacggcc ggggctgcca gtaacctcac 1921 caccggttac ggaagtacag gtaccgcagg tcacgagagt ttcatcattg cgggttatgg 1981 aagtacacag acagcgggcc acaaaagtat cctgaccgct ggttatggca gtactcagac 2041 ggccagggac ggtagcgacc tgattgcggg ctatggcagt acgggaaccg caggctcggg 2101 cagttcgctg atcgcaggtt atggcagcac ccagaccgcg agttacagaa gcatgctgac 2161 cgccggttat ggcagtaccc agaccgccag agaacacagc gaccttgtca caggctatgg 2221 cagcacttca acggcagggt caaacagttc gctgatcgcc ggctatggaa gcactcagac 2281 ggcgggcttc aaaagcatac tgaccgccgg ttacggcagt acccagacgg cacaggagcg 2341 cacgagcctg gtcgcaggct acggaagcac gtcgactgcg ggctattcca gttccttgat 2401 cgccggctat ggcagcacgc agacggcagg ctacgaaagc acgttgaccg ccggttacgg 2461 cagtacgcaa accgctcagg aaaacagctc gctcaccaca ggttacggaa gtacttctac 2521 tgcgggctat tccagctcgc tcatcgcggg ttacggcagt acgcaaacgg caggctacga 2581 gagcacgttg accgccggtt acggtagtac gcaaaccgcg caggagcgca gtgatctggt 2641 gacaggttat ggaagtacct ccaccgccgg ctatgcgagc tcgctgattg cgggttatgg 2701 cagcacgcag actgcgggtt atgagagcac gttgaccgcc ggttacggca gcacgcaaac 2761 cgcacaggaa aacagctcgc tcaccaccgg gtacggaagt acctccacag ccggctttgc 2821 cagctcgctg atctccggtt atggcagtac gcagacagcc ggctataaaa gtaccctcac 2881 ggccggttac ggcagtactc agaccgcaga gtatggaagc tcactcactg cgggctacgg 2941 cagcactgca acggccgggc aggacagttc attgatagcc ggctatggca gctccctgac 3001 cagcggaatc agaagttttc tgacggcagg ctatggcagt acgctgatcg ccggacttcg 3061 cagcgttttg atcgccggtt atggcagtag tcttacatcg ggcgttcgca gcacgttgac 3121 tgcgggttat ggcagtaacc agattgcaag ttacggcagc tcgttgattg caggccatga 3181 aagcattcag gtcgccggaa ataaaagcat gctgatcgcc ggcaagggca gctcgcagac 3241 agcaggtttt cgcagcacgc tgattgccgg tgcgggcagt gtacaactgg cgggtgatcg 3301 cagccggttg attgccggtg cagacagtaa tcagaccgcg ggtgaccgca gcaaactgct 3361 ggccggtaat aacagttatc tgactgccgg cgatagaagc aaactgaccg gcgggcatga 3421 ctgcaccctg atggcgggag accaaagcag attgaccgct ggtaagaaca gtgtcttgac 3481 ggcaggcgct cgtagcaaac tgattggcag tgaaggctcg acgctctcgg ctggagaaga 3541 ctccatactt attttcaggc tctgggacgg gaagaggtac aggcaactgg tcgccagaac 3601 gggtgagaac ggtgttgagg ccgacatacc gtattacgtg aacgaagatg acgatattgt 3661 cgataaaccc gacgaggacg atgactggat agaggtaaag tagtactaga gccaggcatc 3721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //