BBa_K584028
type: ComponentDefinition
persistent identity: BBa K584028
display id: BBa_K584028
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-Lux hybrid promoter + INP(Ice Nucleating Protein Generator)
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:47Z
owned by: james
owned by: myers
mutable description: The pLac-Lux hybrid promoter is repressed by the lacI repressor and induced by IPTG. It is repressed by the luxR repressor bound to the corepressor CO6HSL. It contains a lux box and a lacI binding site. The "Design Notes" portion of this page contains a truth table for this logic gate. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein. We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''. To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.
discontinued: false
dominant: false
experience: experience/None
group u list: _755_
m user id: 0
owner id: 9860
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K584024
component: BBa_K091100
sequence annotation: BBa_K091100
sequence annotation: BBa_K584024
sequence: https://synbiohub.org/public/igem/BBa_K584028_sequence/1

BBa_K584024
type: Component
persistent identity: component2133446
display id: component2133446
version: 1
top level: BBa_K584028
definition: BBa_K584024
access: public

BBa_K091100
type: Component
persistent identity: component2133434
display id: component2133434
version: 1
top level: BBa_K584028
definition: BBa_K091100
access: public

BBa_K091100
type: SequenceAnnotation
persistent identity: annotation2133434
display id: annotation2133434
version: 1
top level: BBa_K584028
location: https://synbiohub.org/public/igem/BBa_K584028/annotation2133434/range2133434/1
component: BBa_K091100

https://synbiohub.org/public/igem/BBa_K584028/annotation2133434/range2133434/1
type: Range
persistent identity: range2133434
display id: range2133434
version: 1
top level: BBa_K584028
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_K584024
type: SequenceAnnotation
persistent identity: annotation2133446
display id: annotation2133446
version: 1
top level: BBa_K584028
location: https://synbiohub.org/public/igem/BBa_K584028/annotation2133446/range2133446/1
component: BBa_K584024

https://synbiohub.org/public/igem/BBa_K584028/annotation2133446/range2133446/1
type: Range
persistent identity: range2133446
display id: range2133446
version: 1
top level: BBa_K584028
direction: direction/reverse
start: 83
end: 3840
orientation: inline

BBa_K091100
type: ComponentDefinition
persistent identity: BBa K091100
display id: BBa_K091100
version: 1
was generated by: iGEM to SBOL conversion
description: pLac_lux hybrid promoter
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: R0010 modified with the insertion of a lux box in between -35 and -10.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This promoter is repressed by lacI and luxR with HSL. It contains a lux box and a lacI binding site.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Deleted the CAP binding site in R0010.
star: true
creator: Pallavi Penumetcha
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: lux box
sequence annotation: -10
sequence annotation: lacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K091100_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1963333
display id: annotation1963333
version: 1
top level: BBa_K091100
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
type: Range
persistent identity: range1963333
display id: range1963333
version: 1
top level: BBa_K091100
direction: direction/forward
start: 10
end: 15
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1963335
display id: annotation1963335
version: 1
top level: BBa_K091100
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
type: Range
persistent identity: range1963335
display id: range1963335
version: 1
top level: BBa_K091100
direction: direction/forward
start: 16
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963334
display id: annotation1963334
version: 1
top level: BBa_K091100
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
type: Range
persistent identity: range1963334
display id: range1963334
version: 1
top level: BBa_K091100
direction: direction/forward
start: 34
end: 39
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963336
display id: annotation1963336
version: 1
top level: BBa_K091100
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
type: Range
persistent identity: range1963336
display id: range1963336
version: 1
top level: BBa_K091100
direction: direction/forward
start: 40
end: 74
orientation: inline

BBa_K584024
type: ComponentDefinition
persistent identity: BBa K584024
display id: BBa_K584024
version: 1
was generated by: iGEM to SBOL conversion
description: Ice Nucleating Protein Generator (RBS + InaZ [K584021] + Terminator)
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:47Z
owned by: james
owned by: myers
mutable description: This part is the Ice Nucleating protien generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _755_
m user id: 0
owner id: 9860
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0015
component: BBa_K584021
sequence annotation: BBa_B0034
sequence annotation: BBa_K584021
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K584024_sequence/1

BBa_B0034
type: Component
persistent identity: component2130281
display id: component2130281
version: 1
top level: BBa_K584024
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2130290
display id: component2130290
version: 1
top level: BBa_K584024
definition: BBa_B0015
access: public

BBa_K584021
type: Component
persistent identity: component2130283
display id: component2130283
version: 1
top level: BBa_K584024
definition: BBa_K584021
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2130281
display id: annotation2130281
version: 1
top level: BBa_K584024
location: https://synbiohub.org/public/igem/BBa_K584024/annotation2130281/range2130281/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K584024/annotation2130281/range2130281/1
type: Range
persistent identity: range2130281
display id: range2130281
version: 1
top level: BBa_K584024
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K584021
type: SequenceAnnotation
persistent identity: annotation2130283
display id: annotation2130283
version: 1
top level: BBa_K584024
location: https://synbiohub.org/public/igem/BBa_K584024/annotation2130283/range2130283/1
component: BBa_K584021

https://synbiohub.org/public/igem/BBa_K584024/annotation2130283/range2130283/1
type: Range
persistent identity: range2130283
display id: range2130283
version: 1
top level: BBa_K584024
direction: direction/reverse
start: 19
end: 3621
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2130290
display id: annotation2130290
version: 1
top level: BBa_K584024
location: https://synbiohub.org/public/igem/BBa_K584024/annotation2130290/range2130290/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K584024/annotation2130290/range2130290/1
type: Range
persistent identity: range2130290
display id: range2130290
version: 1
top level: BBa_K584024
direction: direction/reverse
start: 3630
end: 3758
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K584021
type: ComponentDefinition
persistent identity: BBa K584021
display id: BBa_K584021
version: 1
was generated by: iGEM to SBOL conversion
description: Ice Nucleating Protein Gene - inaZ
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:47Z
owned by: james
owned by: myers
mutable description: The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria [1] This part contains the Ice nuceating protein coding sequence. References [1] Amarjeet S. Bassi1 et.al, Biotechnol. Prog. 2000, 16, 557-563
discontinued: false
dominant: false
experience: experience/None
group u list: _755_
m user id: 0
owner id: 9860
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: InaZ
sequence: https://synbiohub.org/public/igem/BBa_K584021_sequence/1

InaZ
type: SequenceAnnotation
persistent identity: annotation2130178
display id: annotation2130178
version: 1
top level: BBa_K584021
location: https://synbiohub.org/public/igem/BBa_K584021/annotation2130178/range2130178/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K584021/annotation2130178/range2130178/1
type: Range
persistent identity: range2130178
display id: range2130178
version: 1
top level: BBa_K584021
direction: direction/forward
start: 1
end: 3603
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K584028_sequence/1
type: Sequence
persistent identity: BBa K584028 sequence
display id: BBa_K584028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accccaggcttgacacctgtaggatcgtacaggtataattgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaatctcgacaaggcgttggtgctgcgtacctgtgcaaataacatggccgatcactgcggccttatatggcccgcgtccggcacggtggaatccagatactggcagtcaaccaggcggcatgagaatggtctggtcggtttactgtggggcgctggaaccagcgcttttctaagcgtgcatgccgatgctcgatggattgtctgtgaagttgccgttgcagacatcatcagtctggaagagccgggaatggtcaagtttccgcgggccgaggtggttcatgtcggcgacaggatcagcgcgtcacacttcatttcggcacgtcaggccgaccctgcgtcaacatcaacatcaacgttaacgccaatgcccactgccatacccacgcccatgcctgccgtagcaagtgtcacgttaccggtggccgaacaggcccgtcatgaagtgttcgatgtcgcgtcggtcagcgcggctgccgccccagtaaacaccctgccggtgacgacgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgagaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091100_sequence/1
type: Sequence
persistent identity: BBa K091100 sequence
display id: BBa_K091100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accccaggcttgacacctgtaggatcgtacaggtataattgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K584024_sequence/1
type: Sequence
persistent identity: BBa K584024 sequence
display id: BBa_K584024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaatctcgacaaggcgttggtgctgcgtacctgtgcaaataacatggccgatcactgcggccttatatggcccgcgtccggcacggtggaatccagatactggcagtcaaccaggcggcatgagaatggtctggtcggtttactgtggggcgctggaaccagcgcttttctaagcgtgcatgccgatgctcgatggattgtctgtgaagttgccgttgcagacatcatcagtctggaagagccgggaatggtcaagtttccgcgggccgaggtggttcatgtcggcgacaggatcagcgcgtcacacttcatttcggcacgtcaggccgaccctgcgtcaacatcaacatcaacgttaacgccaatgcccactgccatacccacgcccatgcctgccgtagcaagtgtcacgttaccggtggccgaacaggcccgtcatgaagtgttcgatgtcgcgtcggtcagcgcggctgccgccccagtaaacaccctgccggtgacgacgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgagaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K584021_sequence/1
type: Sequence
persistent identity: BBa K584021 sequence
display id: BBa_K584021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatctcgacaaggcgttggtgctgcgtacctgtgcaaataacatggccgatcactgcggccttatatggcccgcgtccggcacggtggaatccagatactggcagtcaaccaggcggcatgagaatggtctggtcggtttactgtggggcgctggaaccagcgcttttctaagcgtgcatgccgatgctcgatggattgtctgtgaagttgccgttgcagacatcatcagtctggaagagccgggaatggtcaagtttccgcgggccgaggtggttcatgtcggcgacaggatcagcgcgtcacacttcatttcggcacgtcaggccgaccctgcgtcaacatcaacatcaacgttaacgccaatgcccactgccatacccacgcccatgcctgccgtagcaagtgtcacgttaccggtggccgaacaggcccgtcatgaagtgttcgatgtcgcgtcggtcagcgcggctgccgccccagtaaacaccctgccggtgacgacgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgagaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z