LOCUS BBa_K590012 3220 bp DNA linear 25-Nov-2024 DEFINITION pGA4C5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert ACCESSION BBa_K590012 VERSION BBa_K590012.1 FEATURES Location/Qualifiers misc_feature 1..15 /label=pGA Suffix misc_feature 16..21 /label=BgIBrK Suffix, XhoI stem_loop 56..88 /label=terminator stem loop primer_bind 116..135 /label=Vr misc_feature 143..178 /label=3K3 Pre-Prefix misc_feature 3200..3220 /label=pGA Prefix ORIGIN 1 ggatccaaca gggttctcga ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagctgtcag accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac 241 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 301 tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata ccgctgcctt 361 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg 421 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac 481 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc 541 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg 601 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag 661 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg 721 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc 781 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt 841 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa 901 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag 961 cagaatttac agatacccac aactcaaagg aaaaggacat gtaattatca ttgactagcc 1021 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta 1081 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc 1141 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa 1201 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa 1261 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc 1321 aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca 1381 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa 1441 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg 1501 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag 1561 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa 1621 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg 1681 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta 1741 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac 1801 aaaataccaa caaccattac atcagattcc tacctacgta acggactaag aaaaacacta 1861 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac 1921 atgcaaagta agcatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc 1981 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc 2041 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttagatatc 2101 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga 2161 gcttttacga gtttttggtg catttaaagc tgttcaccat gaacagatcg acaatgtaac 2221 tactagaggt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca 2281 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag 2341 aaaaaaatca cgggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 2401 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 2461 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 2521 cttgcccgcc tgatgaacgc tcacccggag tttcgtatgg ccatgaaaga cggtgagctg 2581 gtgatctggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 2641 tcgtccctct ggagtgaata ccacgacgat ttccggcagt ttctccacat atattcgcaa 2701 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 2761 ttttttgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 2821 atggacaact tcttcgcccc cgttttcacg atgggcaaat attatacgca aggcgacaag 2881 gtgctgatgc cgctggcgat ccaggttcat catgccgttt gtgatggctt ccatgtcggc 2941 cgcatgctta atgaattaca acagtactgt gatgagtggc agggcggggc gtaataatac 3001 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa 3061 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg 3121 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt 3181 agttagccct tagtgactcg aattcctgct gcggagatct //