LOCUS BBa_K590013 3391 bp DNA linear 22-Nov-2024 DEFINITION pGA4A5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert ACCESSION BBa_K590013 VERSION BBa_K590013.1 FEATURES Location/Qualifiers ORIGIN 1 ggatccaaca gggttctcga ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagctgtcag accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac 241 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 301 tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata ccgctgcctt 361 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg 421 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac 481 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc 541 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg 601 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag 661 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg 721 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc 781 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt 841 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa 901 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag 961 cagaatttac agatacccac aactcaaagg aaaaggacat gtaattatca ttgactagcc 1021 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta 1081 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc 1141 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa 1201 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa 1261 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc 1321 aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca 1381 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa 1441 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg 1501 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag 1561 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa 1621 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg 1681 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta 1741 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac 1801 aaaataccaa caaccattac atcagattcc tacctacgta acggactaag aaaaacacta 1861 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac 1921 atgcaaagta agcatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc 1981 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc 2041 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttagatatc 2101 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga 2161 gcttttacga gtttttggtg catttaaagc tgttcaccat gaacagatcg acaatgtaac 2221 tactagagct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 2281 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 2341 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 2401 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 2461 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 2521 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 2581 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 2641 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 2701 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 2761 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 2821 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 2881 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 2941 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 3001 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 3061 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 3121 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaata ctagctccgg 3181 caaaaaaacg ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact 3241 tcgcgtttgc cacctgacgt ctaagaaaag gaatattcag caatttgccc gtgccgaaga 3301 aaggcccacc cgtgaaggtg agccagtgag ttgattgcta cgtaattagt tagttagccc 3361 ttagtgactc gaattcctgc tgcggagatc t //