LOCUS       BBa_K590015             1855 bp    DNA     linear       26-Jan-2026
DEFINITION  sfGFP_mamK_pGA1C3
ACCESSION   BBa_K590015
VERSION     BBa_K590015.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    60..63
                     /label=conserved
     RBS             56..67
                     /label=BBa_B0034
     misc_feature    68..70
                     /label=start
     CDS             68..787
                     /label=superfolder GFP coding region
     misc_feature    782..787
                     /label=stop
     CDS             68..787
                     /label=BBa_I746916
     CDS             788..811
                     /label=protein
     misc_feature    788..811
                     /label=BBa_K590082
     CDS             812..1855
                     /label=BBa_K590080
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacaaaaga
       61 ggagaaaatg cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact
      121 ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac
      181 taatggtaaa ctgacgctga agttcatctg tactactggt aaactgccgg taccttggcc
      241 gactctggta acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat
      301 gaagcagcat gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat
      361 ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac
      421 cctggtaaac cgcattgagc tgaaaggcat tgactttaaa gaagacggca atatcctggg
      481 ccataagctg gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa
      541 aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct
      601 ggctgatcac taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa
      661 tcactatctg agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat
      721 ggttctgctg gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa
      781 atgatgaggc tctagcggtg gcagctctgg tatgagtgaa ggtgaaggcc aggccaagaa
      841 cagattgttc cttggcatcg accttgggac ttcccatact gcggtgatgt cgagccgggg
      901 caaaaagttc ctgctgaagt cggtggttgg atacccgaag gacgtcatcg gtttgaagct
      961 gctcggccgc ccctatgtgg tcggtgacga ggctttcgag atgcgctcct acctggacat
     1021 ccgctatccg ctccaggacg gcgtgctgag cgagatcagc gaccgcgaca tcgaagtggc
     1081 gcggcatctg ctgacccatg tggtgaagtc ggccgagccc ggccccaacg acgagatctg
     1141 cgccgtcatc ggcgtgccgg cgcgggcgtc ggcggccaac aaggcgctgc tgctcaagat
     1201 ggctcaggag gtggttcata ccgccctagt ggtgtccgaa ccgttcatgg tcggctacgg
     1261 cctggacaag ctgatcaaca ccatcatcgt cgatatcggc gccggaacca cggatatctg
     1321 cgccctgaag ggcactgttc ccggccccga agatcaggtt accctgacca aggctggcaa
     1381 ctatgtggat gagcggctcc agaacgccat cttggaacgt catcccgaac tgcagatgaa
     1441 cgtgaatgtc gcctgcgcgg tgaaagagca gttctccttc gtcggcaccc ccaccgaggt
     1501 tgcctcgttc gagttccgcg ccgccggcaa gcccgtgcgc gctgacgtga ccgagccggt
     1561 gaagatcgcc tgcgaggcgc tgatgcccga cattatcgaa agcatcgaga cgctgctgcg
     1621 ttcgttccag cccgaatatc aggccaccgt gcttcaaaac atcgtgtttg ccggcggcgg
     1681 ctcgcgcatt cgcggattgg cggcctacgt caaggaaaag ctgcgtccct tcggcgatgc
     1741 gaacgtgacc tgcgtcaagg accccacttt cgacggttgc cggggcgcct tgcgtctggc
     1801 ggaagagctt ccgccgcagt attggcgtca gcttggagac gtctccggct cgtga
//