BBa_K314100
1
BBa_K314100
High constitutive expression cassette
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
construct
Released HQ 2013
High constitutive protein expression insert includes f1 origin and RBS B0034
false
false
_496_
0
6395
9
In stock
true
false
Tomas Huber, Justin Siegel
component2090995
1
BBa_K314110
annotation2090995
1
BBa_K314110
range2090995
1
1
455
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K590031
1
ADC
Aldehyde Decarbonylase (ADC) - Synechococcus elongatus PCC7942
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Ordered from GenScript
Produces the enzyme that removes the carbonyl group (C=O) from the fatty aldehyde, yielding an alkane one carbon shorter than the original Acyl-ACP and a molecule of formate
false
false
_761_
0
10712
9
Not in stock
true
Aldehyde decarbonylase gene from synechococcus elongatus
false
Lei Zheng
BBa_K590025
1
PetroBrick
The PetroBrick: Strong Constitutive Expression of ADC and AAR in pSB1C3
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Gene-synthesis
Synechococcus elongatus PCC7942 Aldehyde Decarbonylase rbs Acyl-ACP Reductase in PSB1C3 high consititutive vector for alkane production
false
true
_761_
0
10712
9
It's complicated
true
None
false
Lei Zheng
component2129103
1
BBa_B0034
component2129104
1
BBa_K590032
component2129101
1
BBa_K590031
component2129100
1
BBa_K314100
annotation2129100
1
BBa_K314100
range2129100
1
1
518
annotation2129103
1
BBa_B0034
range2129103
1
1289
1300
annotation2129104
1
BBa_K590032
range2129104
1
1307
2392
annotation2129101
1
BBa_K590031
range2129101
1
525
1280
BBa_K590032
1
AAR
Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Ordered from GenScript
Produces enzyme that reduces long chain length acyl-ACPs into the corresponding fatty aldehydes
false
false
_761_
0
10712
9
Not in stock
true
None
false
Lei Zheng
BBa_K590030
1
BBa_K590030
DrR-FabH2-PSB1C3-High constitutive
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Gene-synthesis
Released HQ 2013
Aldehyde Decarbonylase rbs Acyl-ACP Reductase FabH2 in PSB1C3 high consititutive vector for branched alkane production
false
false
_761_
0
10712
9
In stock
false
None
false
Matthew Harger, Lei Zheng
component2129215
1
BBa_B0034
component2129216
1
BBa_K590034
component2129213
1
BBa_K590025
annotation2129213
1
BBa_K590025
range2129213
1
1
2392
annotation2129216
1
BBa_K590034
range2129216
1
2419
3441
annotation2129215
1
BBa_B0034
range2129215
1
2401
2412
BBa_K314110
1
f1 ori
f1 origin
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
Pet 29 vector
Released HQ 2013
Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
false
false
_496_
0
6395
9
In stock
true
false
IGEM 2010 Team Washington
annotation2115768
1
f1 origin
range2115768
1
18
324
BBa_K590034
1
FabH2
FabH2
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
TBD
Released HQ 2013
Makes Branched Chain Alkanes
false
false
_761_
0
10712
9
In stock
true
None
false
Matthew Harger
BBa_K314110_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K590025_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctactcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggtagtacggaaaatctgtactttcaatccggtgcactcgagcaccaccaccaccaccactgatactagagaaagaggagaaatactagatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga
BBa_K314100_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
BBa_K590032_sequence
1
atgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga
BBa_K590030_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctactcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggtagtacggaaaatctgtactttcaatccggtgcactcgagcaccaccaccaccaccactgatactagagaaagaggagaaatactagatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactgatactagagaaagaggagaaatactagatgtctaaagcgaaaatcaccgcgatcggcacctacgcgccgtctcgtcgcctcaccaacgcggacctcgaaaaaatcgtggacacgtctgacgagtggatcgttcagcgtaccggcatgcgtgaacgtcgtatcgcggacgaacaccagttcacctctgacctgtgcatcgaagcggttaaaaacctgaaatctcgttacaaaggtactctggacgatgttgacatgatcctggttgcgaccaccaccagcgactacgcgttcccgtctaccgcgtgccgcgttcaggagtacttcggttgggaatctaccggtgcgctcgacatcaacgctacgtgcgcaggcctcacttacggtctccacctggcgaatggcctgattacctctggcctgcaccagaagattctggttattgctggtgagactctctctaaagttaccgactataccgaccgtaccacctgtgttctcttcggtgacgccgcaggcgcgctgctcgtggagcgtgacgaggaaactccgggtttcctggcgtctgtgcagggtactagcggtaacggtggtgatatcctgtaccgtgcgggtctgcgtaacgaaatcaacggtgtacagctggttggttccggtaaaatggttcagaacggtcgtgaagtttacaaatgggccgctcgtaccgttccgggtgaatttgaacgcctgctgcacaaagcgggcctgtctagcgacgatctggactggtttgttccgcactctgcgaacctgcgtatgatcgaatctatctgcgagaaaaccccgttccctatcgagaagaccctgacctctgttgaacactacggtaacacctcttctgtttctatcgttctggcgctggatctggcggtgaaagcaggcaaactgaaaaaagaccaaattgttctgctgttcggtttcggcggtggtctgacttacaccggtctgctgatcaaatggggtatgggtggtagcggtggttctgactacaaagacgacgatgacaaataatga
BBa_K590034_sequence
1
atgtctaaagcgaaaatcaccgcgatcggcacctacgcgccgtctcgtcgcctcaccaacgcggacctcgaaaaaatcgtggacacgtctgacgagtggatcgttcagcgtaccggcatgcgtgaacgtcgtatcgcggacgaacaccagttcacctctgacctgtgcatcgaagcggttaaaaacctgaaatctcgttacaaaggtactctggacgatgttgacatgatcctggttgcgaccaccaccagcgactacgcgttcccgtctaccgcgtgccgcgttcaggagtacttcggttgggaatctaccggtgcgctcgacatcaacgctacgtgcgcaggcctcacttacggtctccacctggcgaatggcctgattacctctggcctgcaccagaagattctggttattgctggtgagactctctctaaagttaccgactataccgaccgtaccacctgtgttctcttcggtgacgccgcaggcgcgctgctcgtggagcgtgacgaggaaactccgggtttcctggcgtctgtgcagggtactagcggtaacggtggtgatatcctgtaccgtgcgggtctgcgtaacgaaatcaacggtgtacagctggttggttccggtaaaatggttcagaacggtcgtgaagtttacaaatgggccgctcgtaccgttccgggtgaatttgaacgcctgctgcacaaagcgggcctgtctagcgacgatctggactggtttgttccgcactctgcgaacctgcgtatgatcgaatctatctgcgagaaaaccccgttccctatcgagaagaccctgacctctgttgaacactacggtaacacctcttctgtttctatcgttctggcgctggatctggcggtgaaagcaggcaaactgaaaaaagaccaaattgttctgctgttcggtttcggcggtggtctgacttacaccggtctgctgatcaaatggggtatgggtggtagcggtggttctgactacaaagacgacgatgacaaataatga
BBa_K590031_sequence
1
atgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctactcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggtagtacggaaaatctgtactttcaatccggtgcactcgagcaccaccaccaccaccactga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z