LOCUS BBa_K590045 2730 bp DNA linear 14-Nov-2024 DEFINITION AAR-PSB3K3-Lac Inducible ACCESSION BBa_K590045 VERSION BBa_K590045.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 CDS 1645..2730 /label=BBa_K590032 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatgttt ggcctgatcg gtcatctgac ctcactggaa 1681 caggcgcgtg atgtgtcgcg tcgcatgggt tatgatgaat acgccgacca gggcctggaa 1741 ttttggagca gcgcaccgcc gcaaattgtc gatgaaatca ccgtgacgag tgccacgggt 1801 aaagttattc acggccgtta tatcgaatcc tgctttctgc cggaaatgct ggcggcccgt 1861 cgcttcaaaa ccgcaacgcg caaagtcctg aacgctatga gccatgcgca gaaacacggt 1921 attgatatca gtgcactggg cggttttacc tccattatct ttgaaaactt cgatctggcg 1981 tctctgcgtc aggtgcgcga caccacgctg gaatttgaac gtttcaccac gggtaatacc 2041 catacggcct acgttatttg ccgccaggtc gaagcagctg cgaaaaccct gggcattgat 2101 atcacccaag ccacggttgc agtggttggc gctacgggtg acatcggctc tgcggtctgt 2161 cgttggctgg atctgaaact gggcgtgggt gacctgattc tgaccgctcg taaccaggaa 2221 cgcctggata atctgcaagc cgaactgggc cgcggtaaaa tcctgccgct ggaagcagca 2281 ctgccggaag ccgactttat tgtgtgggtt gcaagcatgc cgcagggtgt cgtgatcgat 2341 ccggcgaccc tgaaacaacc gtgtgtgctg attgacggcg gttatccgaa aaacctgggc 2401 agtaaagtgc agggcgaagg tatttacgtt ctgaatggcg gtgttgtcga acactgcttc 2461 gatattgact ggcagatcat gagcgctgcg gaaatggctc gtccggaacg ccaaatgttt 2521 gcgtgtttcg ccgaagcaat gctgctggaa tttgaaggtt ggcataccaa cttctcatgg 2581 ggccgtaatc agattacgat cgaaaaaatg gaagccatcg gtgaagcatc ggttcgccac 2641 ggctttcagc cgctggctct ggcaattggt agcaccgaaa atctgtattt ccaatctggc 2701 gcgctcgagc accaccacca ccaccactga //