BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2002
1
-10
range2002
1
43
48
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
annotation2001
1
lac O1
range2001
1
26
42
annotation1999
1
lac O1
range1999
1
3
19
BBa_K314111
1
BBa_K314111
Lac I
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
Pet 29 vector
Lac I gene with promoter and RBS
false
false
_496_
0
6395
9
It's complicated
false
false
IGEM 2010 Team Washington
BBa_K590032
1
AAR
Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Ordered from GenScript
Produces enzyme that reduces long chain length acyl-ACPs into the corresponding fatty aldehydes
false
false
_761_
0
10712
9
Not in stock
true
None
false
Lei Zheng
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K314110
1
f1 ori
f1 origin
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
Pet 29 vector
Released HQ 2013
Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
false
false
_496_
0
6395
9
In stock
true
false
IGEM 2010 Team Washington
annotation2115768
1
f1 origin
range2115768
1
18
324
BBa_K590045
1
BBa_K590045
AAR-PSB3K3-Lac Inducible
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Gene-synthesis
Synechococcus elongatus PCC7942 Acyl-ACP Reductase (AAR) in PSB3K3 high consititutive vector
false
false
_761_
0
10712
9
It's complicated
false
None
false
Lei Zheng
component2129265
1
BBa_K314103
component2129266
1
BBa_K590032
annotation2129266
1
BBa_K590032
range2129266
1
1645
2730
annotation2129265
1
BBa_K314103
range2129265
1
1
1638
BBa_K314103
1
BBa_K314103
Lac induced expression cassette
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
construct
Released HQ 2013
Lac induced protein expression insert includes f1 origin, Lac I gene and RBS
false
false
_496_
0
6395
9
In stock
true
false
Tomas Huber, Justin Siegel
component2091082
1
BBa_K314111
component2091083
1
BBa_R0011
component2091081
1
BBa_K314110
component2091089
1
BBa_B0034
annotation2091089
1
BBa_B0034
range2091089
1
1627
1638
annotation2091081
1
BBa_K314110
range2091081
1
1
455
annotation2091083
1
BBa_R0011
range2091083
1
1564
1617
annotation2091082
1
BBa_K314111
range2091082
1
464
1555
BBa_K314110_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K590045_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga
BBa_K590032_sequence
1
atgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga
BBa_K314111_sequence
1
gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_K314103_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z