BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation1999 1 lac O1 range1999 1 3 19 BBa_K590032 1 AAR Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942 2011-09-14T11:00:00Z 2015-05-08T01:12:48Z Ordered from GenScript Produces enzyme that reduces long chain length acyl-ACPs into the corresponding fatty aldehydes false false _761_ 0 10712 9 Not in stock true None false Lei Zheng BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K590047 1 BBa_K590047 AAR-PSB4C5-Lac Inducible w/o LacI 2011-09-14T11:00:00Z 2015-05-08T01:12:48Z Gene-synthesis Synechococcus elongatus PCC7942 Acyl-ACP Reductase (AAR) in PSB3K3 Lac inducible w/o LacI nor F1 ori false true _761_ 0 10712 9 It's complicated false None false Austin Moon, Lei Zheng component2129281 1 BBa_B0034 component2129275 1 BBa_R0011 component2129282 1 BBa_K590032 annotation2129282 1 BBa_K590032 range2129282 1 82 1167 annotation2129281 1 BBa_B0034 range2129281 1 64 75 annotation2129275 1 BBa_R0011 range2129275 1 1 54 BBa_K590047_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga BBa_B0034_sequence 1 aaagaggagaaa BBa_K590032_sequence 1 atgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z