LOCUS BBa_K590050 3393 bp DNA linear 22-Nov-2024 DEFINITION ADC-AAR-PSB1C3-Lac Inducible ACCESSION BBa_K590050 VERSION BBa_K590050.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 misc_feature 2401..3423 /label=AAR Protein misc_feature 1678..2370 /label=ADC from Synech misc_feature 2371..2400 /label=Linker CDS 1645..3393 /label=BBa_K590052 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatgccg cagctggaag cgtcactgga actggatttt 1681 caaagcgaat cttataaaga cgcctactcg cgtattaacg caattgtcat cgaaggcgaa 1741 caggaagctt tcgataacta taatcgtctg gcggaaatgc tgccggatca gcgcgacgaa 1801 ctgcataaac tggccaaaat ggaacaacgt cacatgaaag gctttatggc atgcggtaaa 1861 aacctgtccg ttaccccgga tatgggcttt gcccagaaat ttttcgaacg cctgcatgaa 1921 aatttcaaag cggcggcggc ggaaggcaaa gtggttacgt gcctgctgat tcaaagcctg 1981 attatcgaat gttttgcgat cgcggcctat aacatttaca tcccggtggc ggatgccttc 2041 gcacgtaaaa ttaccgaagg cgtcgtgcgt gacgaatatc tgcaccgcaa ctttggtgaa 2101 gaatggctga aagctaattt cgatgctagt aaagcggaac tggaagaagc gaaccgccag 2161 aatctgccgc tggtttggct gatgctgaat gaagtcgccg atgacgcacg tgaactgggc 2221 atggaacgcg aaagcctggt ggaagacttt atgatcgctt acggcgaagc gctggaaaac 2281 attggtttca ccacgcgtga aatcatgcgc atgtctgcct atggcctggc agctgttggc 2341 gggtcaggcg ggggctcagg cgggtcaatg tttggcctga tcggtcatct gacctcactg 2401 gaacaggcgc gtgatgtgtc gcgtcgcatg ggttatgatg aatacgccga ccagggcctg 2461 gaattttgga gcagcgcacc gccgcaaatt gtcgatgaaa tcaccgtgac gagtgccacg 2521 ggtaaagtta ttcacggccg ttatatcgaa tcctgctttc tgccggaaat gctggcggcc 2581 cgtcgcttca aaaccgcaac gcgcaaagtc ctgaacgcta tgagccatgc gcagaaacac 2641 ggtattgata tcagtgcact gggcggtttt acctccatta tctttgaaaa cttcgatctg 2701 gcgtctctgc gtcaggtgcg cgacaccacg ctggaatttg aacgtttcac cacgggtaat 2761 acccatacgg cctacgttat ttgccgccag gtcgaagcag ctgcgaaaac cctgggcatt 2821 gatatcaccc aagccacggt tgcagtggtt ggcgctacgg gtgacatcgg ctctgcggtc 2881 tgtcgttggc tggatctgaa actgggcgtg ggtgacctga ttctgaccgc tcgtaaccag 2941 gaacgcctgg ataatctgca agccgaactg ggccgcggta aaatcctgcc gctggaagca 3001 gcactgccgg aagccgactt tattgtgtgg gttgcaagca tgccgcaggg tgtcgtgatc 3061 gatccggcga ccctgaaaca accgtgtgtg ctgattgacg gcggttatcc gaaaaacctg 3121 ggcagtaaag tgcagggcga aggtatttac gttctgaatg gcggtgttgt cgaacactgc 3181 ttcgatattg actggcagat catgagcgct gcggaaatgg ctcgtccgga acgccaaatg 3241 tttgcgtgtt tcgccgaagc aatgctgctg gaatttgaag gttggcatac caacttctca 3301 tggggccgta atcagattac gatcgaaaaa atggaagcca tcggtgaagc atcggttcgc 3361 cacggctttc agccgctggc tctggcaatt tga //