BBa_K590050 1 CR-1C3-Lac ADC-AAR-PSB1C3-Lac Inducible 2011-09-14T11:00:00Z 2015-05-08T01:12:48Z Gene-synthesis Synechococcus elongatus PCC7942 Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) direct gene fusion w/ linker for co-localization in Lac inducible vector false false _761_ 0 10712 9 It's complicated false None false Austin Moon, Casey Ager component2129312 1 BBa_K590052 component2129308 1 BBa_K314103 annotation2129308 1 BBa_K314103 range2129308 1 1 1638 annotation2129312 1 BBa_K590052 range2129312 1 1645 3393 BBa_K314111 1 BBa_K314111 Lac I 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Lac I gene with promoter and RBS false false _496_ 0 6395 9 It's complicated false false IGEM 2010 Team Washington BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K314110 1 f1 ori f1 origin 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Released HQ 2013 Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage false false _496_ 0 6395 9 In stock true false IGEM 2010 Team Washington annotation2115768 1 f1 origin range2115768 1 18 324 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation1999 1 lac O1 range1999 1 3 19 annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2001 1 lac O1 range2001 1 26 42 annotation2000 1 -35 range2000 1 20 25 BBa_K314103 1 BBa_K314103 Lac induced expression cassette 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z construct Released HQ 2013 Lac induced protein expression insert includes f1 origin, Lac I gene and RBS false false _496_ 0 6395 9 In stock true false Tomas Huber, Justin Siegel component2091089 1 BBa_B0034 component2091081 1 BBa_K314110 component2091083 1 BBa_R0011 component2091082 1 BBa_K314111 annotation2091089 1 BBa_B0034 range2091089 1 1627 1638 annotation2091082 1 BBa_K314111 range2091082 1 464 1555 annotation2091083 1 BBa_R0011 range2091083 1 1564 1617 annotation2091081 1 BBa_K314110 range2091081 1 1 455 BBa_K590052 1 ADC-AAR Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion 2011-09-14T11:00:00Z 2015-05-08T01:12:48Z Gene-synthesis Synechococcus elongatus PCC7942 Acyl-ACP Reductase to Aldehyde Decarbonylase (ADC-AAR) direct gene fusion w/ linker for co-localization false false _761_ 0 10712 9 Not in stock false None false Austin Moon, Casey Ager, Ingrid Swanson, Seth Sagulo annotation2129090 1 ADC from Synech range2129090 1 34 726 annotation2129089 1 AAR Protein range2129089 1 757 1779 annotation2129091 1 Linker range2129091 1 727 756 BBa_K314110_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt BBa_B0034_sequence 1 aaagaggagaaa BBa_K590052_sequence 1 atgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctactcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggcgggtcaggcgggggctcaggcgggtcaatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaatttga BBa_K314111_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga BBa_K590050_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctactcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggcgggtcaggcgggggctcaggcgggtcaatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaatttga BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K314103_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z