BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K314110
1
f1 ori
f1 origin
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
Pet 29 vector
Released HQ 2013
Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
false
false
_496_
0
6395
9
In stock
true
false
IGEM 2010 Team Washington
annotation2115768
1
f1 origin
range2115768
1
18
324
BBa_K590057
1
9A1
RrD_Y21W-PSB1C3-High constitutive
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Gene-synthesis
Released HQ 2013
Plasmid that consists of Acyl-ACP Reductase rbs mutant Synechococcus elongatus PCC7942 Aldehyde Decarbonylase in PSB1C3 high consititutive vector for alkane production
false
false
_761_
0
10712
9
In stock
false
TBD
false
Lei Zheng, Ben Mo, Elaine Lai, Juhye An
component2129369
1
BBa_B0034
component2129366
1
BBa_K314100
component2129370
1
BBa_K590040
component2129367
1
BBa_K590032
annotation2129366
1
BBa_K314100
range2129366
1
1
518
annotation2129367
1
BBa_K590032
range2129367
1
525
1610
annotation2129369
1
BBa_B0034
range2129369
1
1619
1630
annotation2129370
1
BBa_K590040
range2129370
1
1637
2392
BBa_K590032
1
AAR
Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
Ordered from GenScript
Produces enzyme that reduces long chain length acyl-ACPs into the corresponding fatty aldehydes
false
false
_761_
0
10712
9
Not in stock
true
None
false
Lei Zheng
BBa_K314100
1
BBa_K314100
High constitutive expression cassette
2010-10-07T11:00:00Z
2015-05-08T01:11:55Z
construct
Released HQ 2013
High constitutive protein expression insert includes f1 origin and RBS B0034
false
false
_496_
0
6395
9
In stock
true
false
Tomas Huber, Justin Siegel
component2090995
1
BBa_K314110
annotation2090995
1
BBa_K314110
range2090995
1
1
455
BBa_K590040
1
ADC_Y21W
Aldehyde Decarbonylase_Y21W
2011-09-14T11:00:00Z
2015-05-08T01:12:48Z
In-House Mutant
A mutated Aldehyde Decarbonylase as an enzyme aimed to produce C13 alkane.
false
false
_761_
0
10712
9
Not in stock
false
Aldehyde Decarbonylase has a point mutation at residue 21 from Y to W.
false
Benjamin Mo
BBa_K314110_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K314100_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
BBa_K590032_sequence
1
atgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactga
BBa_K590057_sequence
1
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtttggcctgatcggtcatctgacctcactggaacaggcgcgtgatgtgtcgcgtcgcatgggttatgatgaatacgccgaccagggcctggaattttggagcagcgcaccgccgcaaattgtcgatgaaatcaccgtgacgagtgccacgggtaaagttattcacggccgttatatcgaatcctgctttctgccggaaatgctggcggcccgtcgcttcaaaaccgcaacgcgcaaagtcctgaacgctatgagccatgcgcagaaacacggtattgatatcagtgcactgggcggttttacctccattatctttgaaaacttcgatctggcgtctctgcgtcaggtgcgcgacaccacgctggaatttgaacgtttcaccacgggtaatacccatacggcctacgttatttgccgccaggtcgaagcagctgcgaaaaccctgggcattgatatcacccaagccacggttgcagtggttggcgctacgggtgacatcggctctgcggtctgtcgttggctggatctgaaactgggcgtgggtgacctgattctgaccgctcgtaaccaggaacgcctggataatctgcaagccgaactgggccgcggtaaaatcctgccgctggaagcagcactgccggaagccgactttattgtgtgggttgcaagcatgccgcagggtgtcgtgatcgatccggcgaccctgaaacaaccgtgtgtgctgattgacggcggttatccgaaaaacctgggcagtaaagtgcagggcgaaggtatttacgttctgaatggcggtgttgtcgaacactgcttcgatattgactggcagatcatgagcgctgcggaaatggctcgtccggaacgccaaatgtttgcgtgtttcgccgaagcaatgctgctggaatttgaaggttggcataccaacttctcatggggccgtaatcagattacgatcgaaaaaatggaagccatcggtgaagcatcggttcgccacggctttcagccgctggctctggcaattggtagcaccgaaaatctgtatttccaatctggcgcgctcgagcaccaccaccaccaccactgatactagagaaagaggagaaatactagatgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctggtcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggtagtacggaaaatctgtactttcaatccggtgcactcgagcaccaccaccaccaccactga
BBa_K590040_sequence
1
atgccgcagctggaagcgtcactggaactggattttcaaagcgaatcttataaagacgcctggtcgcgtattaacgcaattgtcatcgaaggcgaacaggaagctttcgataactataatcgtctggcggaaatgctgccggatcagcgcgacgaactgcataaactggccaaaatggaacaacgtcacatgaaaggctttatggcatgcggtaaaaacctgtccgttaccccggatatgggctttgcccagaaatttttcgaacgcctgcatgaaaatttcaaagcggcggcggcggaaggcaaagtggttacgtgcctgctgattcaaagcctgattatcgaatgttttgcgatcgcggcctataacatttacatcccggtggcggatgccttcgcacgtaaaattaccgaaggcgtcgtgcgtgacgaatatctgcaccgcaactttggtgaagaatggctgaaagctaatttcgatgctagtaaagcggaactggaagaagcgaaccgccagaatctgccgctggtttggctgatgctgaatgaagtcgccgatgacgcacgtgaactgggcatggaacgcgaaagcctggtggaagactttatgatcgcttacggcgaagcgctggaaaacattggtttcaccacgcgtgaaatcatgcgcatgtctgcctatggcctggcagctgttggtagtacggaaaatctgtactttcaatccggtgcactcgagcaccaccaccaccaccactga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z