LOCUS BBa_K590062 4302 bp DNA linear 22-Nov-2024 DEFINITION Gli1-ADC HivC-AAR Construct ACCESSION BBa_K590062 VERSION BBa_K590062.1 FEATURES Location/Qualifiers misc_feature 1..456 /label=F1 origin CDS 463..1555 /label=LacI promoter 1564..1617 /label=Lac promoter RBS 1627..1638 /label=BBa_0034 CDS 1639..2166 /label=GLI-1 CDS 2167..2196 /label=linker CDS 2197..2934 /label=ADC RBS 2937..2948 /label=BBa_0034 CDS 2950..3204 /label=HIV1 CDS 3205..3234 /label=linker CDS 3235..4302 /label=AAR ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaaat gaagcgtgag cctgaatctg tgtatgaaac tgactgccgt 1681 tgggatggct gtagccagga atttgactcc caagagcagc tggtgcacca catcaacagc 1741 gagcacatcc acggggagcg gaaggagttc gtgtgccact gggggggctg ctccagggag 1801 ctgaggccct tcaaagccca gtacatgctg gtggttcaca tgcgcagaca cactggcgag 1861 aagccacaca agtgcacgtt tgaagggtgc cggaagtcat actcacgcct cgaaaacctg 1921 aagacgcacc tgcggtcaca cacgggtgag aagccataca tgtgtgagca cgagggctgt 1981 agtaaagcct tcagcaatgc cagtgaccga gccaagcacc agaatcggac ccattccaat 2041 gagaagccgt atgtatgtaa gctccctggc tgcaccaaac gctatacaga tcctagctcg 2101 ctgcgaaaac atgtcaagac agtgcatggt cctgacgccc atgtgaccaa acggcaccgt 2161 ggggatggcg ggtcaggcgg gggctcaggc gggtcaatgc cgcagctgga agcgtcactg 2221 gaactggatt ttcaaagcga atcttataaa gacgcctact cgcgtattaa cgcaattgtc 2281 atcgaaggcg aacaggaagc tttcgataac tataatcgtc tggcggaaat gctgccggat 2341 cagcgcgacg aactgcataa actggccaaa atggaacaac gtcacatgaa aggctttatg 2401 gcatgcggta aaaacctgtc cgttaccccg gatatgggct ttgcccagaa atttttcgaa 2461 cgcctgcatg aaaatttcaa agcggcggcg gcggaaggca aagtggttac gtgcctgctg 2521 attcaaagcc tgattatcga atgttttgcg atcgcggcct ataacattta catcccggtg 2581 gcggatgcct tcgcacgtaa aattaccgaa ggcgtcgtgc gtgacgaata tctgcaccgc 2641 aactttggtg aagaatggct gaaagctaat ttcgatgcta gtaaagcgga actggaagaa 2701 gcgaaccgcc agaatctgcc gctggtttgg ctgatgctga atgaagtcgc cgatgacgca 2761 cgtgaactgg gcatggaacg cgaaagcctg gtggaagact ttatgatcgc ttacggcgaa 2821 gcgctggaaa acattggttt caccacgcgt gaaatcatgc gcatgtctgc ctatggcctg 2881 gcagctgttg gtagtacgga aaatctgtac tttcaatccg gtgcactcga gtgacgaaag 2941 aggagaaata tgcccttcca gtgtcgcatt tgcatgcgga acttttcgct caggacggac 3001 cttgacaggc atacccgtac tcataccggt gaaaaaccgt ttcagtgtcg gatctgtatg 3061 cgaaatttct ccctcagcca gaccttgcgc cgccatctac gtacgcacac cggcgagaag 3121 ccattccaat gccgaatatg catgcgcaac ttcagtctca ggagcaacct ggggcggcac 3181 ctaaaaaccc acacaggaga aaaaggtggc agcggcggtg gctcaggcgg gagcatgttt 3241 ggcctgatcg gtcatctgac ctcactggaa caggcgcgtg atgtgtcgcg tcgcatgggt 3301 tatgatgaat acgccgacca gggcctggaa ttttggagca gcgcaccgcc gcaaattgtc 3361 gatgaaatca ccgtgacgag tgccacgggt aaagttattc acggccgtta tatcgaatcc 3421 tgctttctgc cggaaatgct ggcggcccgt cgcttcaaaa ccgcaacgcg caaagtcctg 3481 aacgctatga gccatgcgca gaaacacggt attgatatca gtgcactggg cggttttacc 3541 tccattatct ttgaaaactt cgatctggcg tctctgcgtc aggtgcgcga caccacgctg 3601 gaatttgaac gtttcaccac gggtaatacc catacggcct acgttatttg ccgccaggtc 3661 gaagcagctg cgaaaaccct gggcattgat atcacccaag ccacggttgc agtggttggc 3721 gctacgggtg acatcggctc tgcggtctgt cgttggctgg atctgaaact gggcgtgggt 3781 gacctgattc tgaccgctcg taaccaggaa cgcctggata atctgcaagc cgaactgggc 3841 cgcggtaaaa tcctgccgct ggaagcagca ctgccggaag ccgactttat tgtgtgggtt 3901 gcaagcatgc cgcagggtgt cgtgatcgat ccggcgaccc tgaaacaacc gtgtgtgctg 3961 attgacggcg gttatccgaa aaacctgggc agtaaagtgc agggcgaagg tatttacgtt 4021 ctgaatggcg gtgttgtcga acactgcttc gatattgact ggcagatcat gagcgctgcg 4081 gaaatggctc gtccggaacg ccaaatgttt gcgtgtttcg ccgaagcaat gctgctggaa 4141 tttgaaggtt ggcataccaa cttctcatgg ggccgtaatc agattacgat cgaaaaaatg 4201 gaagccatcg gtgaagcatc ggttcgccac ggctttcagc cgctggctct ggcaattggt 4261 agcaccgaaa atctgtattt ccaatctggc gcgctcgagt ga //