LOCUS BBa_K590063 12390 bp DNA linear 26-Nov-2024 DEFINITION mamHIEJKL_pLacGFP_pGA3K3 ACCESSION BBa_K590063 VERSION BBa_K590063.1 FEATURES Location/Qualifiers misc_feature 85..1380 /label=mamH misc_feature 1417..1626 /label=mamI misc_feature 25..71 /label=mamAB Promoter CDS 1..4291 /label=BBa_K590000 misc_feature 4328..6514 /label=mamE CDS 4300..6552 /label=BBa_K590001 misc_feature 6630..8150 /label=mamJ CDS 6561..8186 /label=BBa_K590002 misc_feature 8242..9285 /label=mamK misc_feature 9341..9577 /label=mamL CDS 8195..9633 /label=BBa_K590003 misc_feature 9642..9662 /label=pGA suffix misc_feature 9663..9734 /label=E. coli his operon terminator stem_loop 9671..9705 /label=terminator stem loop primer_bind 9798..9817 /label=Vr CDS 9880..10695 /label=kanR CDS 11027..11851 /label=p15A ori primer_bind 12252..12271 /label=Vf2 stem_loop 12341..12360 /label=terminator stem loop misc_feature 12370..12390 /label=pGA prefix misc_feature 9642..12390 /label=BBa_K590014 ORIGIN 1 ggtaccttcg tatgaaccct gtgtttatgt gttgtcaacc gcccccgact cgctgttata 61 gtctgaccga atcggaggtg acgggtgtct cgagtggaag cggctgcggc agaagttaaa 121 gtgcgccagc acaacgccct ttatttgctg tcggcgttgt gcatggtttt catgaccctg 181 gtggttgcta ttcagccgct attcttgcga aacgttctta atatctcatt cgaaaccgcc 241 ggcgccgtca atgccaatgt gcaggtggtg accgaggttc tggatctctt catcttcgcc 301 tatctcggct acctgtcgga tcggatcggg cgggtcagga tcatcgtggc cggttttctg 361 gtcgccgcca tcggtgcggt gatcgcgccg ctcagcccct ggatcggcgg tgcctccatc 421 ggggcgctgg tcgtctatta cgtttcgcgg gtcatcatgt cggcgggcag cggcgcggta 481 tggcctcaat tgtcggccct ggccggggat ttcagcgacg acgacacccg ggcgcgcctg 541 atgtccaata ccgcgttcat gatggccttc ggggtgacgc tggtctacgc tgttttgatg 601 cagatcccgg cccatgcggg catcgccgtg accatgctgc tgacggcggc ggtctccctg 661 gccggtgctt ggctggcggg gaaattcctg gtggatgtcg ctccgcgcac ccaggaaacc 721 tcggttccct ggcgggcggt ctggagcttg gtgaaggcgg agccccgcct gcgcctggct 781 ttcgccagtt cgctgttcgc ccgcagcgac atggtgttcg tagggctctt cctcatgctg 841 tggttcatct actttgccga tctgatcaag gtcgggcagg ccgaggcggc ggcgcgggcc 901 ggaatcttga tcgggctgat gggggcggtg gtcatgctct cgatcccggt ttggcggtcg 961 tttatcgagc atttcggccg cattcaggcg gttctgctcg ggatggtgct ctccgccctg 1021 ggattcataa tgttgggttt catcatcaat ccgttcgact ggttcatcgt actgcccatc 1081 ttgttgattg cgtccgggca ggctggttgt tttgtggcgc ctcaaatatt aacagttgac 1141 tacgcgccta gggatttgct tgggtctgtg cttggcgcat ttaatgttat tgggtgcatt 1201 ggtattattt tctttgttca ggtcggcggg tttttatttg attatgttgg cccgccggca 1261 ccttttgtct ttactggtgt tggaaacttg attatatcgg cgtatgcatt gcgcctgcta 1321 aaacgcgagg cgcgtgacgg cggcggcgac gacgccccag gggacgacgg ggtggcgtag 1381 cgccattcgc tctgcatcaa gactggagta acgtttatgc caagcgtgat tttcggactg 1441 ctggcgcttg ccctcggatt gctgggggtg acggcatggt ggtggtcggt gaccgagttc 1501 ctgcgcggag cggtgccggt ggccctgctc atccttggct tggtcgcgtt ggcctccggg 1561 gtgcaatccg tgcggttgcc tcgttccaac aaggggaccg cttcagaccc tgacatcgat 1621 ggttgatggc catgttcaat ggtgacgtag aagactcgga tcgggcgggt caggatcatc 1681 gtggccggtt ttctggtcgc cgccatcggt gcggtgatcg cgccgctcag cccctggatc 1741 ggcggtgcct ccatcggggc gctggtcgtc tattacgttt cgcgggtcat catgtcggcg 1801 ggcagcggcg cggtatggcc tcaattgtcg gccctggccg gggatttcag cgacgacgac 1861 acccgggcgc gcctgatgtc caataccgcg ttcatgatgg ccttcggggt gacgctggtc 1921 tacgctgttt tgatgcagat cccggcccat gcgggcatcg ccgtgaccat gctgctgacg 1981 gcggcggtct ccctggccgg tgcttggctg gcggggaaat tcctggtgga tgtcgctccg 2041 cgcacccagg aaacctcggt tccctggcgg gcggtctgga gcttggtgaa ggcggagccc 2101 cgcctgcgcc tggctttcgc cagttcgctg ttcgcccgca gcgacatggt gttcgtaggg 2161 ctcttcctca tgctgtggtt catctacttt gccgatctga tcaaggtcgg gcaggccgag 2221 gcggcggcgc gggccggaat cttgatcggg ctgatggggg cggtggtcat gctctcgatc 2281 ccggtttggc ggtcgtttat cgagcatttc ggccgcattc aggcggttct gctcgggatg 2341 gtgctctccg ccctgggatt cataatgttg ggtttcatca tcaatccgtt cgactggttc 2401 atcgtactgc ccatcttgtt gattgcgtcc gggcaggctg gttgttttgt ggcgcctcaa 2461 atattaacag ttgactacgc gcctagggat ttgcttgggt ctgtgcttgg cgcatttaat 2521 gttattgggt gcattggtat tattttcttt gttcaggtcg gcgggttttt atttgattat 2581 gttggcccgc cggcaccttt tgtctttact ggtgttggaa acttgattat atcggcgtat 2641 gcattgcgcc tgctaaaacg cgaggcgcgt gacggcggcg gcgacgacgc cccaggggac 2701 gacggggtgg cgtagcgcca ttcgctctgc atcaagactg gagtaacgtt tatgccaagc 2761 gtgattttcg gactgctggc gcttgccctc ggattgctgg gggtgacggc atggtggtgg 2821 tcggtgaccg agttcctgcg cggagcggtg ccggtggccc tgctcatcct tggcttggtc 2881 gcgttggcct ccggggtgca atccgtgcgg ttgcctcgtt ccaacaaggg gaccgcttca 2941 gaccctgaca tcgatggttg atggccatgt tcaatggtga cgtagaagac atcgggcggg 3001 tcaggatcat cgtggccggt tttctggtcg ccgccatcgg tgcggtgatc gcgccgctca 3061 gcccctggat cggcggtgcc tccatcgggg cgctggtcgt ctattacgtt tcgcgggtca 3121 tcatgtcggc gggcagcggc gcggtatggc ctcaattgtc ggccctggcc ggggatttca 3181 gcgacgacga cacccgggcg cgcctgatgt ccaataccgc gttcatgatg gccttcgggg 3241 tgacgctggt ctacgctgtt ttgatgcaga tcccggccca tgcgggcatc gccgtgacca 3301 tgctgctgac ggcggcggtc tccctggccg gtgcttggct ggcggggaaa ttcctggtgg 3361 atgtcgctcc gcgcacccag gaaacctcgg ttccctggcg ggcggtctgg agcttggtga 3421 aggcggagcc ccgcctgcgc ctggctttcg ccagttcgct gttcgcccgc agcgacatgg 3481 tgttcgtagg gctcttcctc atgctgtggt tcatctactt tgccgatctg atcaaggtcg 3541 ggcaggccga ggcggcggcg cgggccggaa tcttgatcgg gctgatgggg gcggtggtca 3601 tgctctcgat cccggtttgg cggtcgttta tcgagcattt cggccgcatt caggcggttc 3661 tgctcgggat ggtgctctcc gccctgggat tcataatgtt gggtttcatc atcaatccgt 3721 tcgactggtt catcgtactg cccatcttgt tgattgcgtc cgggcaggct ggttgttttg 3781 tggcgcctca aatattaaca gttgactacg cgcctaggga tttgcttggg tctgtgcttg 3841 gcgcatttaa tgttattggg tgcattggta ttattttctt tgttcaggtc ggcgggtttt 3901 tatttgatta tgttggcccg ccggcacctt ttgtctttac tggtgttgga aacttgatta 3961 tatcggcgta tgcattgcgc ctgctaaaac gcgaggcgcg tgacggcggc ggcgacgacg 4021 ccccagggga cgacggggtg gcgtagcgcc attcgctctg catcaagact ggagtaacgt 4081 ttatgccaag cgtgattttc ggactgctgg cgcttgccct cggattgctg ggggtgacgg 4141 catggtggtg gtcggtgacc gagttcctgc gcggagcggt gccggtggcc ctgctcatcc 4201 ttggcttggt cgcgttggcc tccggggtgc aatccgtgcg gttgcctcgt tccaacaagg 4261 ggaccgcttc agaccctgac atcgatggtt gtactagagc cgcttcagac cctgacatcg 4321 atggttgatg gccatgttca atggtgacgt agaagacggc gggcgcggcg atgcttcctg 4381 cggcaaggat ctgaagcgct atctgatgct catgggcgtg gttgccctgg tcgtcctatt 4441 tggcgccttc atttaccggc agtcctcggg cggtcttcgt ctcggcgcca tgttggagca 4501 gatggggcgg ggcaccggcc ctgccgtcaa tgttccggta cagcagggcg ggccgtcggc 4561 agccgtcaat ccggccatgt cggttccggc tggtgcccgc gtggcgccgc cctcggccgc 4621 cggtgctata gcgaccatgc ctcccatggt ggatttcgga ccggctccca ttggggccgg 4681 ggggccgttc tccagcgtcg tcaccttgct gcgcaacagc gtcgtcgccg tgaccgcgtc 4741 atcggccaac gggcaggcca tgcccgatcc cctgggcttg gccaaccctg acggactgcc 4801 ccatttcgcc aatcccgcca cccggtcggt ggaaaacatc ggcaccggcg tgatcgtccg 4861 taacgacggc ttcatcgtca ccaactatca tgtggtgcgc ggcgccaatt cggtgttcgt 4921 taccgtgcag gacgacgtgg gctcgacccg ctattccgcc gaaatcatca agatggacga 4981 ggctctggat ctcgccttgc tgaaggtcgc ccccaagacg ccgttgacag ccgccgttct 5041 gggcgacagc gacggggtgc aggtcgccga cgaagtgatc gccatcggga ctcccttcgg 5101 tctggacatg accgtcagcc gcggcatcat ttccgccaag cgcaagtcca tggtgatcga 5161 gggcgtgacc cattccaacc tgctgcagac cgacgccgcc atcaaccagg gcaattccgg 5221 cggtccgctg gtgatttcca acgggacggt ggtcggcatc aataccgcca tctacacccc 5281 caacggcgcc tttgccggaa taggcttcgc cgtgcccagc aatcaggccc gcctgttcat 5341 cttggatgaa gtgggctggc tgccgacctc caccgccgag ggggcctcca tgggcctggt 5401 ggccatgcag cgccccatgg gcggcggcgt gggcgcggcg ggtccggcca ttttcgccgg 5461 gactagggcg ccccacaccg atggccgcca gaacatggat tgcaccacct gccacgatct 5521 gattccggcc ggaaacgggc ggcccgcgcc gatgatgccc atcgccgccc cgattccgcc 5581 gccgccgatt cccatgggcg ccgtctcgcc ccataccgac ggccgtcaga acatgaactg 5641 cgccaattgc catcagatgc tgggcggtgc cgcgcccatc gccgcaccgg gactcggcgg 5701 gggggcttac cgcttcgcgc agccgccggg aagcctggcc atcaatattc aggggccgcg 5761 cggcgggcag tcgaccgccg ccggaaccgg ccgggtcacg cttttgggcg ccgccttgac 5821 tcccatgtcg cagcgccttg gcgcccagac cggcgtaccg gtcggacgcg gagtgttcat 5881 ctccggcgtc acgcccaata ctccggcggc gacggcgggg ctgcggcccg gcgacgtctt 5941 gctcaaggtg gacggccgtc ctgtccgcct gccggaagaa gtctccgcca tcatggtcga 6001 gatgcatgcc ggacggtcgg tgcggctcgg cgtcctgcgc gatggcgatg tgcgcaacat 6061 gaccctggtc gcgggacccg ccggtctggc cgccgcagcg gtccaggctc cggccattgc 6121 cgacatggcg cagccgccca tgggcggcat ggcccccact gcacccggca tggtcgcggt 6181 tcccggtggt ccggccgtca tgccgaagcc tccgaccgag ttcaactggc tgggcatgga 6241 gatcgaaacc ttccaggcgc cgcgtccgat caccggtgtg ccgggcgccg ttccggttcc 6301 cggagccaag ggtgcccagg tggccgaggt tctggtcggg tcccgggccg ccgtggcggg 6361 gctccaggcc aatgacctca tcctcgaggt caataatcgg ccggtggccg gtccggctcg 6421 tctcgacgcc gccattaagg gggcgacgaa tgccgggcag cagattttgc tgaaggtcaa 6481 ccgcaatggc caagagttct ggattgtcct ttgatggtgg agctggataa tggcgaataa 6541 ccggcgagat cgtactagag cagattttgc tgaaggtcaa ccgcaatggc caagagttct 6601 ggattgtcct ttgatggtgg agctggataa tggcgaataa ccggcgagat cgcgacaaag 6661 gcgacgggag ccagggtgaa ggcttgtcgg cgggcggcgg aatgccgtcg gagcccgaga 6721 tcgtctcggt gacggtccat cccacgccaa ctttggccgt gtcgctgaag ccggcgcagc 6781 aaggcgatat ttgggccagt ttgctggaaa gctcgccctg gtcggccaat cacggcggcc 6841 ggatcgagcc ggttcagccg agccccgccc ctacccgccc cacggaatcc ccgtccgtcg 6901 gcgatctggt cagccgctgg tcgcagccga tctggcggac ccccagccgc gtggaagagg 6961 tcgagacccc ggagagcaat ttcgtcatcg agttggaggc gtcccagccg atcacttcgg 7021 atatcacggt gtccgtctcc gatctggtga tcgaggttca gccgatccag accaccgaag 7081 ccgagccgga gcccgagctt ccggtggccg agcccgagcc cggagtcgtg ccggtggacg 7141 ttgtggccga agccgagccg gagcccgagc ttccggtggc cgagcccgag cccggagtcg 7201 tgccggtgga cgttgtggcc gaagccgagc cggagccgga gcttccggtg gccgagcccg 7261 agcccgaagt cgtgccggtg gacgttgtgg ccgaagccga gccggagccg gagcttccgg 7321 tggccgagcc cgagcccgaa gtcgtgccgg tggacgttgt ggccgaagcc gagccggagc 7381 cggagcttcc ggtggccgag cccgagcccg aagtcgtgcc ggtggacgtt gtggccgaag 7441 ccgagccgga gccggagctt ccggtggccg cgcccgagcc cgaagtcttg ccggtggagg 7501 ttgtggccga agccgagccg gagccggagc ttccggtggc cgcgcccgag cccgaagtct 7561 tgccggtgga ggttgtggcc gaagccgagc cggagcccga gcttccggtg gccgcgcccg 7621 agcccgaagt cgtgccggtg gacgttgtgg ccgaagccga gccggagccc gagcttccgg 7681 tggccgagcc cgaattggtt catgaggcgg ctgtttccga gccggtggcc gaaagcatcg 7741 aggacgcggt ggcggttccc gccccggcga ctaatttcgg tcttgccgaa ctgatgcagg 7801 cggctgcggc catggccccg gttttggcct ctcccccttc cgttgcccca gcggccaagc 7861 ccgtccgaga gcccgcgaag aagaggaagg ctccgccggt cagaaagaag gtcgccacgg 7921 ttcaggacgt tcccgtcgaa gaccttctcg gtggaatttt cgggatcgcc ggttcggcgg 7981 tgcgtagtgt cctcagtctt ggtgccgggg tcgtcggcgg cgtggtcaag ggggggcgtg 8041 tcattgggga caacgtcgtc gccggtgcgc gtcggcttac ggggtccgcc gagggaagtt 8101 gcgggacctg ctccacgtct caatgcgata cggtgggcaa gaaaaaatag ctggcagccg 8161 tcaattggtc atttagcacg cgaacgtact agagctggca gccgtcaatt ggtcatttag 8221 cacgcgaacg gagtgacaaa aatgagtgaa ggtgaaggcc aggccaagaa cagattgttc 8281 cttggcatcg accttgggac ttcccatact gcggtgatgt cgagccgggg caaaaagttc 8341 ctgctgaagt cggtggttgg atacccgaag gacgtcatcg gtttgaagct gctcggccgc 8401 ccctatgtgg tcggtgacga ggctttcgag atgcgctcct acctggacat ccgctatccg 8461 ctccaggacg gcgtgctgag cgagatcagc gaccgcgaca tcgaagtggc gcggcatctg 8521 ctgacccatg tggtgaagtc ggccgagccc ggccccaacg acgagatctg cgccgtcatc 8581 ggcgtgccgg cgcgggcgtc ggcggccaac aaggcgctgc tgctcaagat ggctcaggag 8641 gtggttcata ccgccctagt ggtgtccgaa ccgttcatgg tcggctacgg cctggacaag 8701 ctgatcaaca ccatcatcgt cgatatcggc gccggaacca cggatatctg cgccctgaag 8761 ggcactgttc ccggccccga agatcaggtt accctgacca aggctggcaa ctatgtggat 8821 gagcggctcc agaacgccat cttggaacgt catcccgaac tgcagatgaa cgtgaatgtc 8881 gcctgcgcgg tgaaagagca gttctccttc gtcggcaccc ccaccgaggt tgcctcgttc 8941 gagttccgcg ccgccggcaa gcccgtgcgc gctgacgtga ccgagccggt gaagatcgcc 9001 tgcgaggcgc tgatgcccga cattatcgaa agcatcgaga cgctgctgcg ttcgttccag 9061 cccgaatatc aggccaccgt gcttcaaaac atcgtgtttg ccggcggcgg ctcgcgcatt 9121 cgcggattgg cggcctacgt caaggaaaag ctgcgtccct tcggcgatgc gaacgtgacc 9181 tgcgtcaagg accccacttt cgacggttgc cggggcgcct tgcgtctggc ggaagagctt 9241 ccgccgcagt attggcgtca gcttggagac gtctccggct cgtgacgggg ggagagttcg 9301 atatgcggga cggggatacg cggcagtcta ggacagtggc atggtaagat tgatcggatc 9361 gttggtgttc ggcgggttga tcgtgctgtt ggcgtcgtcc aacgcgcata tggtcgaaac 9421 ccgcctggga ccactggcga tgattgcgcc gcatttcgtt gttctgggca taacgttttt 9481 tctcggtttc gccatcggca tcgtctcggt gctggcgaac gtcatgacgc ggcgcaagca 9541 gaagtcgccg gggaagagca tcgtcatcaa gcgctgaatc ccttcgcttg ggttgaaggc 9601 ggggtcgggg ctttggagtg agtaaggatg aggtactaga gggatccaac agggttctcg 9661 agtccggcaa aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa 9721 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 9781 tgcggcgagc ggtatcagct cactcaaagg cggtaatctc gagtcccgtc aagtcagcgt 9841 aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat 9901 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg 9961 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta 10021 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa 10081 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa 10141 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa 10201 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac 10261 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac 10321 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc 10381 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg 10441 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt 10501 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt 10561 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata 10621 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg 10681 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt 10741 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg 10801 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga 10861 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa 10921 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc 10981 ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctagc ggagtgtata 11041 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa 11101 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc 11161 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg 11221 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc 11281 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa 11341 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc tggcggctcc 11401 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg 11461 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga 11521 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct 11581 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt 11641 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg 11701 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct 11761 tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac 11821 caaaacgatc tcaagaagat catcttatta aggggtctga cgctcagtgg aacgaaaact 11881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 11941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 12001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 12061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 12121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 12181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 12241 ctattccatg gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 12301 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga 12361 tgatttctgg aattcctgct gcggagatct //