##gff-version 3
##sequence-region BBa_K590063 1 12390
BBa_K590063	.	CDS	8195	9633	.	+	0	ID=BBa_K590003;Name=BBa_K590003
BBa_K590063	.	sequence_feature	8242	9285	.	+	0	ID=annotation2138181;Name=mamK;Parent=BBa_K590003
BBa_K590063	.	sequence_feature	9341	9577	.	+	0	ID=annotation2138182;Name=mamL;Parent=BBa_K590003
BBa_K590063	.	plasmid_vector	9642	12390	.	+	0	ID=BBa_K590014;Name=BBa_K590014
BBa_K590063	.	stem_loop	9671	9705	.	+	0	ID=annotation2145071;Name=terminator stem loop;Parent=BBa_K590014
BBa_K590063	.	CDS	9880	10695	.	+	0	ID=annotation2145073;Name=kanR;Parent=BBa_K590014
BBa_K590063	.	sequence_feature	12370	12390	.	+	0	ID=annotation2145077;Name=pGA prefix;Parent=BBa_K590014
BBa_K590063	.	primer_binding_site	12252	12271	.	+	0	ID=annotation2145075;Name=Vf2;Parent=BBa_K590014
BBa_K590063	.	engineered_region	9642	9662	.	+	0	ID=annotation2145069;Name=pGA suffix;Parent=BBa_K590014
BBa_K590063	.	CDS	11027	11851	.	+	0	ID=annotation2145074;Name=p15A ori;Parent=BBa_K590014
BBa_K590063	.	sequence_feature	9663	9734	.	+	0	ID=annotation2145070;Name=E. coli his operon terminator;Parent=BBa_K590014
BBa_K590063	.	primer_binding_site	9798	9817	.	+	0	ID=annotation2145072;Name=Vr;Parent=BBa_K590014
BBa_K590063	.	stem_loop	12341	12360	.	+	0	ID=annotation2145076;Name=terminator stem loop;Parent=BBa_K590014
BBa_K590063	.	CDS	6561	8186	.	+	0	ID=BBa_K590002;Name=BBa_K590002
BBa_K590063	.	sequence_feature	6630	8150	.	+	0	ID=annotation2138180;Name=mamJ;Parent=BBa_K590002
BBa_K590063	.	CDS	1	4291	.	+	0	ID=BBa_K590000;Name=BBa_K590000
BBa_K590063	.	sequence_feature	1417	1626	.	+	0	ID=annotation2138082;Name=mamI;Parent=BBa_K590000
BBa_K590063	.	sequence_feature	85	1380	.	+	0	ID=annotation2138081;Name=mamH ;Parent=BBa_K590000
BBa_K590063	.	sequence_feature	25	71	.	+	0	ID=annotation2138176;Name=mamAB Promoter;Parent=BBa_K590000
BBa_K590063	.	CDS	4300	6552	.	+	0	ID=BBa_K590001;Name=BBa_K590001
BBa_K590063	.	sequence_feature	4328	6514	.	+	0	ID=annotation2138177;Name=mamE;Parent=BBa_K590001
>BBa_K590063
ggtaccttcgtatgaaccctgtgtttatgtgttgtcaaccgcccccgactcgctgttatagtctgaccgaatcggaggt
gacgggtgtctcgagtggaagcggctgcggcagaagttaaagtgcgccagcacaacgccctttatttgctgtcggcgtt
gtgcatggttttcatgaccctggtggttgctattcagccgctattcttgcgaaacgttcttaatatctcattcgaaacc
gccggcgccgtcaatgccaatgtgcaggtggtgaccgaggttctggatctcttcatcttcgcctatctcggctacctgt
cggatcggatcgggcgggtcaggatcatcgtggccggttttctggtcgccgccatcggtgcggtgatcgcgccgctcag
cccctggatcggcggtgcctccatcggggcgctggtcgtctattacgtttcgcgggtcatcatgtcggcgggcagcggc
gcggtatggcctcaattgtcggccctggccggggatttcagcgacgacgacacccgggcgcgcctgatgtccaataccg
cgttcatgatggccttcggggtgacgctggtctacgctgttttgatgcagatcccggcccatgcgggcatcgccgtgac
catgctgctgacggcggcggtctccctggccggtgcttggctggcggggaaattcctggtggatgtcgctccgcgcacc
caggaaacctcggttccctggcgggcggtctggagcttggtgaaggcggagccccgcctgcgcctggctttcgccagtt
cgctgttcgcccgcagcgacatggtgttcgtagggctcttcctcatgctgtggttcatctactttgccgatctgatcaa
ggtcgggcaggccgaggcggcggcgcgggccggaatcttgatcgggctgatgggggcggtggtcatgctctcgatcccg
gtttggcggtcgtttatcgagcatttcggccgcattcaggcggttctgctcgggatggtgctctccgccctgggattca
taatgttgggtttcatcatcaatccgttcgactggttcatcgtactgcccatcttgttgattgcgtccgggcaggctgg
ttgttttgtggcgcctcaaatattaacagttgactacgcgcctagggatttgcttgggtctgtgcttggcgcatttaat
gttattgggtgcattggtattattttctttgttcaggtcggcgggtttttatttgattatgttggcccgccggcacctt
ttgtctttactggtgttggaaacttgattatatcggcgtatgcattgcgcctgctaaaacgcgaggcgcgtgacggcgg
cggcgacgacgccccaggggacgacggggtggcgtagcgccattcgctctgcatcaagactggagtaacgtttatgcca
agcgtgattttcggactgctggcgcttgccctcggattgctgggggtgacggcatggtggtggtcggtgaccgagttcc
tgcgcggagcggtgccggtggccctgctcatccttggcttggtcgcgttggcctccggggtgcaatccgtgcggttgcc
tcgttccaacaaggggaccgcttcagaccctgacatcgatggttgatggccatgttcaatggtgacgtagaagactcgg
atcgggcgggtcaggatcatcgtggccggttttctggtcgccgccatcggtgcggtgatcgcgccgctcagcccctgga
tcggcggtgcctccatcggggcgctggtcgtctattacgtttcgcgggtcatcatgtcggcgggcagcggcgcggtatg
gcctcaattgtcggccctggccggggatttcagcgacgacgacacccgggcgcgcctgatgtccaataccgcgttcatg
atggccttcggggtgacgctggtctacgctgttttgatgcagatcccggcccatgcgggcatcgccgtgaccatgctgc
tgacggcggcggtctccctggccggtgcttggctggcggggaaattcctggtggatgtcgctccgcgcacccaggaaac
ctcggttccctggcgggcggtctggagcttggtgaaggcggagccccgcctgcgcctggctttcgccagttcgctgttc
gcccgcagcgacatggtgttcgtagggctcttcctcatgctgtggttcatctactttgccgatctgatcaaggtcgggc
aggccgaggcggcggcgcgggccggaatcttgatcgggctgatgggggcggtggtcatgctctcgatcccggtttggcg
gtcgtttatcgagcatttcggccgcattcaggcggttctgctcgggatggtgctctccgccctgggattcataatgttg
ggtttcatcatcaatccgttcgactggttcatcgtactgcccatcttgttgattgcgtccgggcaggctggttgttttg
tggcgcctcaaatattaacagttgactacgcgcctagggatttgcttgggtctgtgcttggcgcatttaatgttattgg
gtgcattggtattattttctttgttcaggtcggcgggtttttatttgattatgttggcccgccggcaccttttgtcttt
actggtgttggaaacttgattatatcggcgtatgcattgcgcctgctaaaacgcgaggcgcgtgacggcggcggcgacg
acgccccaggggacgacggggtggcgtagcgccattcgctctgcatcaagactggagtaacgtttatgccaagcgtgat
tttcggactgctggcgcttgccctcggattgctgggggtgacggcatggtggtggtcggtgaccgagttcctgcgcgga
gcggtgccggtggccctgctcatccttggcttggtcgcgttggcctccggggtgcaatccgtgcggttgcctcgttcca
acaaggggaccgcttcagaccctgacatcgatggttgatggccatgttcaatggtgacgtagaagacatcgggcgggtc
aggatcatcgtggccggttttctggtcgccgccatcggtgcggtgatcgcgccgctcagcccctggatcggcggtgcct
ccatcggggcgctggtcgtctattacgtttcgcgggtcatcatgtcggcgggcagcggcgcggtatggcctcaattgtc
ggccctggccggggatttcagcgacgacgacacccgggcgcgcctgatgtccaataccgcgttcatgatggccttcggg
gtgacgctggtctacgctgttttgatgcagatcccggcccatgcgggcatcgccgtgaccatgctgctgacggcggcgg
tctccctggccggtgcttggctggcggggaaattcctggtggatgtcgctccgcgcacccaggaaacctcggttccctg
gcgggcggtctggagcttggtgaaggcggagccccgcctgcgcctggctttcgccagttcgctgttcgcccgcagcgac
atggtgttcgtagggctcttcctcatgctgtggttcatctactttgccgatctgatcaaggtcgggcaggccgaggcgg
cggcgcgggccggaatcttgatcgggctgatgggggcggtggtcatgctctcgatcccggtttggcggtcgtttatcga
gcatttcggccgcattcaggcggttctgctcgggatggtgctctccgccctgggattcataatgttgggtttcatcatc
aatccgttcgactggttcatcgtactgcccatcttgttgattgcgtccgggcaggctggttgttttgtggcgcctcaaa
tattaacagttgactacgcgcctagggatttgcttgggtctgtgcttggcgcatttaatgttattgggtgcattggtat
tattttctttgttcaggtcggcgggtttttatttgattatgttggcccgccggcaccttttgtctttactggtgttgga
aacttgattatatcggcgtatgcattgcgcctgctaaaacgcgaggcgcgtgacggcggcggcgacgacgccccagggg
acgacggggtggcgtagcgccattcgctctgcatcaagactggagtaacgtttatgccaagcgtgattttcggactgct
ggcgcttgccctcggattgctgggggtgacggcatggtggtggtcggtgaccgagttcctgcgcggagcggtgccggtg
gccctgctcatccttggcttggtcgcgttggcctccggggtgcaatccgtgcggttgcctcgttccaacaaggggaccg
cttcagaccctgacatcgatggttgtactagagccgcttcagaccctgacatcgatggttgatggccatgttcaatggt
gacgtagaagacggcgggcgcggcgatgcttcctgcggcaaggatctgaagcgctatctgatgctcatgggcgtggttg
ccctggtcgtcctatttggcgccttcatttaccggcagtcctcgggcggtcttcgtctcggcgccatgttggagcagat
ggggcggggcaccggccctgccgtcaatgttccggtacagcagggcgggccgtcggcagccgtcaatccggccatgtcg
gttccggctggtgcccgcgtggcgccgccctcggccgccggtgctatagcgaccatgcctcccatggtggatttcggac
cggctcccattggggccggggggccgttctccagcgtcgtcaccttgctgcgcaacagcgtcgtcgccgtgaccgcgtc
atcggccaacgggcaggccatgcccgatcccctgggcttggccaaccctgacggactgccccatttcgccaatcccgcc
acccggtcggtggaaaacatcggcaccggcgtgatcgtccgtaacgacggcttcatcgtcaccaactatcatgtggtgc
gcggcgccaattcggtgttcgttaccgtgcaggacgacgtgggctcgacccgctattccgccgaaatcatcaagatgga
cgaggctctggatctcgccttgctgaaggtcgcccccaagacgccgttgacagccgccgttctgggcgacagcgacggg
gtgcaggtcgccgacgaagtgatcgccatcgggactcccttcggtctggacatgaccgtcagccgcggcatcatttccg
ccaagcgcaagtccatggtgatcgagggcgtgacccattccaacctgctgcagaccgacgccgccatcaaccagggcaa
ttccggcggtccgctggtgatttccaacgggacggtggtcggcatcaataccgccatctacacccccaacggcgccttt
gccggaataggcttcgccgtgcccagcaatcaggcccgcctgttcatcttggatgaagtgggctggctgccgacctcca
ccgccgagggggcctccatgggcctggtggccatgcagcgccccatgggcggcggcgtgggcgcggcgggtccggccat
tttcgccgggactagggcgccccacaccgatggccgccagaacatggattgcaccacctgccacgatctgattccggcc
ggaaacgggcggcccgcgccgatgatgcccatcgccgccccgattccgccgccgccgattcccatgggcgccgtctcgc
cccataccgacggccgtcagaacatgaactgcgccaattgccatcagatgctgggcggtgccgcgcccatcgccgcacc
gggactcggcgggggggcttaccgcttcgcgcagccgccgggaagcctggccatcaatattcaggggccgcgcggcggg
cagtcgaccgccgccggaaccggccgggtcacgcttttgggcgccgccttgactcccatgtcgcagcgccttggcgccc
agaccggcgtaccggtcggacgcggagtgttcatctccggcgtcacgcccaatactccggcggcgacggcggggctgcg
gcccggcgacgtcttgctcaaggtggacggccgtcctgtccgcctgccggaagaagtctccgccatcatggtcgagatg
catgccggacggtcggtgcggctcggcgtcctgcgcgatggcgatgtgcgcaacatgaccctggtcgcgggacccgccg
gtctggccgccgcagcggtccaggctccggccattgccgacatggcgcagccgcccatgggcggcatggcccccactgc
acccggcatggtcgcggttcccggtggtccggccgtcatgccgaagcctccgaccgagttcaactggctgggcatggag
atcgaaaccttccaggcgccgcgtccgatcaccggtgtgccgggcgccgttccggttcccggagccaagggtgcccagg
tggccgaggttctggtcgggtcccgggccgccgtggcggggctccaggccaatgacctcatcctcgaggtcaataatcg
gccggtggccggtccggctcgtctcgacgccgccattaagggggcgacgaatgccgggcagcagattttgctgaaggtc
aaccgcaatggccaagagttctggattgtcctttgatggtggagctggataatggcgaataaccggcgagatcgtacta
gagcagattttgctgaaggtcaaccgcaatggccaagagttctggattgtcctttgatggtggagctggataatggcga
ataaccggcgagatcgcgacaaaggcgacgggagccagggtgaaggcttgtcggcgggcggcggaatgccgtcggagcc
cgagatcgtctcggtgacggtccatcccacgccaactttggccgtgtcgctgaagccggcgcagcaaggcgatatttgg
gccagtttgctggaaagctcgccctggtcggccaatcacggcggccggatcgagccggttcagccgagccccgccccta
cccgccccacggaatccccgtccgtcggcgatctggtcagccgctggtcgcagccgatctggcggacccccagccgcgt
ggaagaggtcgagaccccggagagcaatttcgtcatcgagttggaggcgtcccagccgatcacttcggatatcacggtg
tccgtctccgatctggtgatcgaggttcagccgatccagaccaccgaagccgagccggagcccgagcttccggtggccg
agcccgagcccggagtcgtgccggtggacgttgtggccgaagccgagccggagcccgagcttccggtggccgagcccga
gcccggagtcgtgccggtggacgttgtggccgaagccgagccggagccggagcttccggtggccgagcccgagcccgaa
gtcgtgccggtggacgttgtggccgaagccgagccggagccggagcttccggtggccgagcccgagcccgaagtcgtgc
cggtggacgttgtggccgaagccgagccggagccggagcttccggtggccgagcccgagcccgaagtcgtgccggtgga
cgttgtggccgaagccgagccggagccggagcttccggtggccgcgcccgagcccgaagtcttgccggtggaggttgtg
gccgaagccgagccggagccggagcttccggtggccgcgcccgagcccgaagtcttgccggtggaggttgtggccgaag
ccgagccggagcccgagcttccggtggccgcgcccgagcccgaagtcgtgccggtggacgttgtggccgaagccgagcc
ggagcccgagcttccggtggccgagcccgaattggttcatgaggcggctgtttccgagccggtggccgaaagcatcgag
gacgcggtggcggttcccgccccggcgactaatttcggtcttgccgaactgatgcaggcggctgcggccatggccccgg
ttttggcctctcccccttccgttgccccagcggccaagcccgtccgagagcccgcgaagaagaggaaggctccgccggt
cagaaagaaggtcgccacggttcaggacgttcccgtcgaagaccttctcggtggaattttcgggatcgccggttcggcg
gtgcgtagtgtcctcagtcttggtgccggggtcgtcggcggcgtggtcaagggggggcgtgtcattggggacaacgtcg
tcgccggtgcgcgtcggcttacggggtccgccgagggaagttgcgggacctgctccacgtctcaatgcgatacggtggg
caagaaaaaatagctggcagccgtcaattggtcatttagcacgcgaacgtactagagctggcagccgtcaattggtcat
ttagcacgcgaacggagtgacaaaaatgagtgaaggtgaaggccaggccaagaacagattgttccttggcatcgacctt
gggacttcccatactgcggtgatgtcgagccggggcaaaaagttcctgctgaagtcggtggttggatacccgaaggacg
tcatcggtttgaagctgctcggccgcccctatgtggtcggtgacgaggctttcgagatgcgctcctacctggacatccg
ctatccgctccaggacggcgtgctgagcgagatcagcgaccgcgacatcgaagtggcgcggcatctgctgacccatgtg
gtgaagtcggccgagcccggccccaacgacgagatctgcgccgtcatcggcgtgccggcgcgggcgtcggcggccaaca
aggcgctgctgctcaagatggctcaggaggtggttcataccgccctagtggtgtccgaaccgttcatggtcggctacgg
cctggacaagctgatcaacaccatcatcgtcgatatcggcgccggaaccacggatatctgcgccctgaagggcactgtt
cccggccccgaagatcaggttaccctgaccaaggctggcaactatgtggatgagcggctccagaacgccatcttggaac
gtcatcccgaactgcagatgaacgtgaatgtcgcctgcgcggtgaaagagcagttctccttcgtcggcacccccaccga
ggttgcctcgttcgagttccgcgccgccggcaagcccgtgcgcgctgacgtgaccgagccggtgaagatcgcctgcgag
gcgctgatgcccgacattatcgaaagcatcgagacgctgctgcgttcgttccagcccgaatatcaggccaccgtgcttc
aaaacatcgtgtttgccggcggcggctcgcgcattcgcggattggcggcctacgtcaaggaaaagctgcgtcccttcgg
cgatgcgaacgtgacctgcgtcaaggaccccactttcgacggttgccggggcgccttgcgtctggcggaagagcttccg
ccgcagtattggcgtcagcttggagacgtctccggctcgtgacggggggagagttcgatatgcgggacggggatacgcg
gcagtctaggacagtggcatggtaagattgatcggatcgttggtgttcggcgggttgatcgtgctgttggcgtcgtcca
acgcgcatatggtcgaaacccgcctgggaccactggcgatgattgcgccgcatttcgttgttctgggcataacgttttt
tctcggtttcgccatcggcatcgtctcggtgctggcgaacgtcatgacgcggcgcaagcagaagtcgccggggaagagc
atcgtcatcaagcgctgaatcccttcgcttgggttgaaggcggggtcggggctttggagtgagtaaggatgaggtacta
gagggatccaacagggttctcgagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccga
aaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatc
agctcactcaaaggcggtaatctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaatt
ctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaa
aagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgatt
ccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgag
tgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctc
gtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctg
ttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctg
aatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagt
acggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatca
ttggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcac
ctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctcga
gcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcat
gatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaactt
ttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcac
caactggtccacctacaacaaagctctcatcaaccgtggctccctcactttctggctggatgatggggcgattcaggcc
tggtatgagtcagcaacaccttcttcacgaggcagacctcagcgctagcggagtgtatactggcttactatgttggcac
tgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgataca
ggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacg
gggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccata
ggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagata
ccaggcgtttcccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttat
ggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaac
cccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcacc
actggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggac
aagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccg
ccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttatta
aggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcaccta
gatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc
ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa
ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt
atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatt
ccatggtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa
tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcctgctgcggagatct