Michael Brasino, Alicia Wong, Rashmi Ravichandran
mamHI, from Magnetospirillum magneticum
Extracted from host genome Magnetospirillum magneticum strain AMB-1
AMB# 0961,0962; Gene function: Loss of mamI causes no membrane formation, found localization onto chains
mamE, from Magnetospirillum magneticum
AMB#0963; Gene function: membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
mamJ, from Magnetospirillum magneticum
AMB#0964; Gene function: proper magnetosome chain organization/assembly
mamKL, from Magnetospirillum magneticum
AMB#0965,0966; Gene function: mamK is required for proper magnetosome chain organization; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI; mamL is crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines.
pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert
Backbone that is Gibson reaction friendly (Bgl Brick)
Conversion of the iGEM parts registry to SBOL2.1