BBa_K590087
1
KumaMax
KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H
2011-09-21T11:00:00Z
2015-05-08T01:12:48Z
Engineered but the original protein is form Alicyclobacillus sendaiensis
A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
false
false
_761_
0
5005
9
It's complicated
true
Aimed to increase binding affinity to the immunogenic PQLP peptide found in gluten
false
Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu
BBa_K590087_sequence
1
atgagtgatatggaaaaaccgtggaaagaaggtgaagaagcccgcgcagtgctgcaaggtcatgctcgtgcgcaggcaccgcaagcagtcgataaaggcccggtggcaggtgacgaacgcatggctgttaccgtggttctgcgtcgccagcgtgcaggtgaactggcggcccacgtggaacgtcaagcagctattgctccgcatgcgcgcgaacacctgaaacgtgaagcgtttgcggccagtcatggtgcgtccctggatgactttgccgaactgcgtcgcttcgcagatgctcacggcctggcgctggaccgtgcaaacgttgcagctggcaccgccgttctgtctggtccggtcgatgcaatcaatcgcgcttttggtgtggaactgcgtcatttcgatcacccggacggctcatatcgttcgtacctgggtgaagtcaccgtgccggccagtattgcaccgatgatcgaagcggttctgggcctggatacgcgtccggtcgcccgcccgcattttcgtatgcagcgtcgcgcagaaggcggtttcgaagctcgttcccaagcggcggcaccgaccgcatatacgccgctggatgttgcgcaggcctaccaatttccggaaggtctggacggccagggtcaatgcattgccattatcgaactgggcggtggctatgatgaagcttcactggcgcagtacttcgcgtcgctgggcgtgccggcaccgcaagtcgtgagtgtttccgtcgatggtgcgagcaaccagccgaccggtgatccgtctggtccggacggtgaagtggaactggatatcgaagttgcaggcgctctggcgccgggtgccaaatttgcagtgtatttcgcgccggataccgatgccggttttctggacgcgattaccacggccatccacgatccgacgctgaaaccgagcgttgtctcaatttcgtggagcggcccggaagacagctggacctctgctgcgatcgccgcaatgaaccgtgcgtttctggatgctgcggccctgggtgtgaccgttctggcagctgcgggcgacagcggttctacgggtggcgaacaggacggtctgtatcatgtgcatttcccggccgcatcaccgtacgttctggcgtgcggtggcacgcgcctggtcgcatcgggtggccgtattgcgcaggaaaccgtctggaacgatggtccggacggtggtgcaacgggtggcggtgtgagccgcatcttcccgctgccggcatggcaggaacacgctaacgttccgccgtctgcaaatccgggcgcgagcagcggccgtggtgtcccggatctggctggtaatgcggacccggcaaccggttatgaagtggttattgatggcgaagcaaccgtcatcggcggtacgagcgccgtggcaccgctgtttgctgcgctggttgcgcgtattaaccagaaactgggcaaagcagttggttatctgaatccgaccctgtaccaactgccggcagatgttttccatgacatcacggagggtaacaatgatattgcaaaccgtgcgcagatttatcaagcaggtccgggctgggacccgtgtaccggtctgggttcaccgattggtgtgcgtctgctgcaagcactgctgccgagtgcctcccagccgcaaccgggtagtacggaaaatctgtactttcagtccggcgcgctcgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z