BBa_K590087 1 KumaMax KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H 2011-09-21T11:00:00Z 2015-05-08T01:12:48Z Engineered but the original protein is form Alicyclobacillus sendaiensis A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively. false false _761_ 0 5005 9 It's complicated true Aimed to increase binding affinity to the immunogenic PQLP peptide found in gluten false Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu BBa_K590087_sequence 1 atgagtgatatggaaaaaccgtggaaagaaggtgaagaagcccgcgcagtgctgcaaggtcatgctcgtgcgcaggcaccgcaagcagtcgataaaggcccggtggcaggtgacgaacgcatggctgttaccgtggttctgcgtcgccagcgtgcaggtgaactggcggcccacgtggaacgtcaagcagctattgctccgcatgcgcgcgaacacctgaaacgtgaagcgtttgcggccagtcatggtgcgtccctggatgactttgccgaactgcgtcgcttcgcagatgctcacggcctggcgctggaccgtgcaaacgttgcagctggcaccgccgttctgtctggtccggtcgatgcaatcaatcgcgcttttggtgtggaactgcgtcatttcgatcacccggacggctcatatcgttcgtacctgggtgaagtcaccgtgccggccagtattgcaccgatgatcgaagcggttctgggcctggatacgcgtccggtcgcccgcccgcattttcgtatgcagcgtcgcgcagaaggcggtttcgaagctcgttcccaagcggcggcaccgaccgcatatacgccgctggatgttgcgcaggcctaccaatttccggaaggtctggacggccagggtcaatgcattgccattatcgaactgggcggtggctatgatgaagcttcactggcgcagtacttcgcgtcgctgggcgtgccggcaccgcaagtcgtgagtgtttccgtcgatggtgcgagcaaccagccgaccggtgatccgtctggtccggacggtgaagtggaactggatatcgaagttgcaggcgctctggcgccgggtgccaaatttgcagtgtatttcgcgccggataccgatgccggttttctggacgcgattaccacggccatccacgatccgacgctgaaaccgagcgttgtctcaatttcgtggagcggcccggaagacagctggacctctgctgcgatcgccgcaatgaaccgtgcgtttctggatgctgcggccctgggtgtgaccgttctggcagctgcgggcgacagcggttctacgggtggcgaacaggacggtctgtatcatgtgcatttcccggccgcatcaccgtacgttctggcgtgcggtggcacgcgcctggtcgcatcgggtggccgtattgcgcaggaaaccgtctggaacgatggtccggacggtggtgcaacgggtggcggtgtgagccgcatcttcccgctgccggcatggcaggaacacgctaacgttccgccgtctgcaaatccgggcgcgagcagcggccgtggtgtcccggatctggctggtaatgcggacccggcaaccggttatgaagtggttattgatggcgaagcaaccgtcatcggcggtacgagcgccgtggcaccgctgtttgctgcgctggttgcgcgtattaaccagaaactgggcaaagcagttggttatctgaatccgaccctgtaccaactgccggcagatgttttccatgacatcacggagggtaacaatgatattgcaaaccgtgcgcagatttatcaagcaggtccgggctgggacccgtgtaccggtctgggttcaccgattggtgtgcgtctgctgcaagcactgctgccgagtgcctcccagccgcaaccgggtagtacggaaaatctgtactttcagtccggcgcgctcgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z