LOCUS BBa_K590090 5229 bp DNA linear 25-Nov-2024 DEFINITION LuxCDE Under Lac ACCESSION BBa_K590090 VERSION BBa_K590090.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 RBS 1647..1658 /label=BBa_B0034 CDS 1665..3108 /label=LuxC CDS 1647..3108 /label=BBa_K590059 RBS 3117..3128 /label=BBa_B0034 CDS 3135..4062 /label=LuxD CDS 3117..4062 /label=BBa_K590060 RBS 4071..4082 /label=BBa_B0034 CDS 4089..5229 /label=LuxE CDS 4071..5229 /label=BBa_K590061 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagagaaag aggagaaata ctagatgaac aaatgcatcc 1681 cgatgattat caacggtatg atccaggact tcgacaacta tgcgtacaaa gaagttaagc 1741 tgaataatga caaccgtgtc aaactctctg tgattaccga atcttctgtt tctaaaaccc 1801 tgaacatcaa ggaccgtatc aacctcaacc tgaatcagat tgttaacttt ctgtacacgg 1861 taggccagcg ttggaaatct gaagaataca atcgtcgtcg tacctacatc cgcgaactga 1921 aaacctacct gggttactcc gacgaaatgg cccgtctcga agccaactgg attgcgatgc 1981 tgctctgctc taaatctgcg ctgtacgaca tcgttaacta cgacctgggc tctatccacg 2041 ttctggatga gtggctcccg cgtggcgact gctacgttaa agcgcagcca aaaggcgttt 2101 ctgttcacct gctggcgggt aatgttccgc tctccggtgt tacctctatt ctgcgtgcga 2161 tcctcaccaa gaacgagtgc atcattaaaa cctcttcttc tgatccattc acggcgaacg 2221 cgctggtttc ctcctttatc gacgtcaacg ccgaccaccc gatcacgaag tctatgtctg 2281 ttatgtactg gcctcacgac gaggacatga cgctctctca gcgtatcatg aaccacgccg 2341 atgtggttat cgcgtggggt ggtgacgaag cgatcaaatg ggcggttaaa tactctccgc 2401 cgcacgttga catcctgaaa ttcggtccaa aaaagtccct gtctatcatt gaggcgccga 2461 aggacatcga agcagccgca atgggtgtgg cgcatgacat ctgcttctac gatcagcagg 2521 cgtgcttctc tacccaggac gtttactaca tcggtgataa cctgccactg ttcctgaacg 2581 aactcgaaaa acagctggac cgctacgcga aaatcctccc taaaggctct aacagctttg 2641 acgaaaaagc ggcgttcact ctgaccgaga aggagtctct gttcgcaggc tatgaagttc 2701 gtaaaggtga caaacaagcc tggctgattg ttgtttctcc gaccaactct tttggtaacc 2761 agccgctgag ccgttctgtc tacgttcacc aggtttctga catcaaagaa atcatcccgt 2821 tcgttaataa gaaccgtacg caaaccgttt ctatctaccc gtgggaagcg tctctgaaat 2881 accgtgacaa gctcgcgcgc agcggtgtcg aacgtatcgt tgagtctggt atgaataaca 2941 tcttccgtgt tggtggtgca cacgattctc tgtctccgct ccagtatctg gttcgtttcg 3001 tgtctcacga acgtccgttc aactacacca cgaaagacgt tgcggttgaa atcgagcaga 3061 cccgttatct ggaagaggac aaattcctgg ttttcgttcc gtaataatta ctagagaaag 3121 aggagaaata ctagatgaaa gacgaatctg cgttcttcac tattgaccac attatcaagc 3181 tggacaacgg ccagtctatc cgtgtttggg aaaccctgcc aaagaagaat gtacctgaaa 3241 aaaagcacac catcctcatt gcttctggtt tcgcgcgtcg tatggaccac ttcgcgggtc 3301 tggcagaata cctgtctacc aacggtttcc acgttattcg ttacgactct ctgcatcacg 3361 ttggtctcag ctctggctgt atcaacgagt tcaccatgtc tatcggtaaa aacagcctgc 3421 tcactgttgt tgactggctg accgaccacg gtgttgaacg tatcggcctg attgcggcgt 3481 ctctctccgc gcgtattgcg tacgaagtcg tcaataagat caaactcagc ttcctgatca 3541 ctgcggttgg tgttgtaaac ctgcgtgaca cgctggagaa ggcactggaa tacgactatc 3601 tgcaactgcc gatctctgag ctgccggagg atctcgattt cgaaggccac aacctcggtt 3661 ctgaggtttt tgttaccgac tgcttcaaac acaactggga caccctggac tctaccctca 3721 actctgtcaa aggtctcgct atcccgttca tcgcgtttac cgcgaacgat gattcttggg 3781 ttaaacagtc tgaagttatc gaactgatcg actctatcga atcttctaac tgcaaactgt 3841 actccctgat cggttcctct cacgatctgg gtgaaaacct ggttgtcctg cgtaacttct 3901 accagtctgt taccaaagcg gcgctggcgc tcgacgacgg tctgctggac ctggaaattg 3961 acatcatcga accgcgtttc gaggacgtta cctctatcac cgttaaagaa cgtcgtctca 4021 aaaacgagat tgagaacgaa ctcctggaac tggcgtaata attactagag aaagaggaga 4081 aatactagat gaccgttcac accgaataca aacgtaatca aatcatcgca tcttctgaaa 4141 ttgatgacct gatcttcatg accaaacctc aggaatggtc ctttgaagag cagaaggaga 4201 tccgcgataa gctcgtacgt gaagcgttct acttccacta caaccgtaat gaggaatacc 4261 gtaactactg catcaaccag cacgtttctg acaacctgca cacgatcgac gaaatcccgg 4321 ttttcccgac ctctgtgttt aagtataaga aactccacac ggtaaccgct gaagacattg 4381 aaaactggta cacctcttct ggtactcgtg gtgtcaagtc tcacatcgcg cgtgatcgcc 4441 tgtccatcga acgtctgctg ggttctgtta acttcggtat gaagtacgtt ggtgactggt 4501 tcgagcacca aatggaactg atcaacctcg gcccagaccg ctttaatacc aacaatattt 4561 ggttcaaata tgttatgtct ctcgttgagc tgctgtaccc gacggagttt actgtcgata 4621 atgataagat tgacttcgaa aagaccgtta agcacctgtt ccgtatcaaa aactctaaaa 4681 aggacatttg tctgatcggt ccgccgttct tcgtttacct cctgtgccag tacatgaaag 4741 agaataacat cgagttcaag ggtggtgacc gtgttcacat catcaccggc ggtggttgga 4801 aatctaacca aaacgactct ctggaccgtg ccgacttcaa ccaactcctg atggatacct 4861 ttcagctcga caagattaac cagattcgcg acacctttaa tcaggttgaa ctgaatacct 4921 gctttttcga ggacgagttc cagcgtaaac acgttccgcc ttgggtttat gcacgcgccc 4981 tggacccgga aaccctgaaa ccagttgcgg acggcgaaat cggcctgctc tcttacatgg 5041 atgcgtcttc taccgcgtac ccggcgttca tcgttaccga cgacatcggt attgtgaaag 5101 aaatccgtga accggacccg taccctggcg tcactgttga aatcgttcgt cgcctgaaca 5161 cccgcgcaca gaaaggttgc gcgctgtcta tggcgaacgt gatccagaaa aacatcaaag 5221 actaataat //