LuxC, LuxD and LuxE in a low copy plasmid under the lac promoter. This part codes for a series of enzymes that catalyzes the production of tetradecanal, the C14 aldehyde. This part is to be co-transformed with ADC for the production of alkanes.
Lac induced protein expression insert includes f1 origin, Lac I gene and RBS
LuxC; standalone, E. Coli codon optimized
A synthesized version of the LuxC gene from the Cambridge 2010 LuxBrick
LuxD; standalone, E. Coli codon optimized
A synthesized version of the LuxD gene from the Cambridge 2010 LuxBrick
LuxE; standalone, E. Coli codon optimized
A synthesized version of the LuxE gene from the Cambridge 2010 LuxBrick
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Promoter (lacI regulated, lambda pL hybrid)
represillator of Elowitz and Leibler (2000)
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
Conversion of the iGEM parts registry to SBOL2.1