BBa_K590090
type: ComponentDefinition
persistent identity: BBa K590090
display id: BBa_K590090
version: 1
was generated by: iGEM to SBOL conversion
description: LuxCDE Under Lac
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Fill in later.
owned by: james
owned by: myers
mutable description: LuxC, LuxD and LuxE in a low copy plasmid under the lac promoter. This part codes for a series of enzymes that catalyzes the production of tetradecanal, the C14 aldehyde. This part is to be co-transformed with ADC for the production of alkanes.
discontinued: false
dominant: false
experience: experience/None
group u list: _761_
m user id: 0
owner id: 7700
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Fill in later.
star: false
creator: Chris Choe, David Zong
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K314103
component: BBa_K590060
component: BBa_K590059
component: BBa_K590061
sequence annotation: BBa_K314103
sequence annotation: BBa_K590059
sequence annotation: BBa_K590060
sequence annotation: BBa_K590061
sequence: https://synbiohub.org/public/igem/BBa_K590090_sequence/1

BBa_K314103
type: Component
persistent identity: component2160578
display id: component2160578
version: 1
top level: BBa_K590090
definition: BBa_K314103
access: public

BBa_K590060
type: Component
persistent identity: component2160584
display id: component2160584
version: 1
top level: BBa_K590090
definition: LuxD
access: public

BBa_K590059
type: Component
persistent identity: component2160581
display id: component2160581
version: 1
top level: BBa_K590090
definition: LuxC
access: public

BBa_K590061
type: Component
persistent identity: component2160587
display id: component2160587
version: 1
top level: BBa_K590090
definition: LuxE
access: public

BBa_K314103
type: SequenceAnnotation
persistent identity: annotation2160578
display id: annotation2160578
version: 1
top level: BBa_K590090
location: https://synbiohub.org/public/igem/BBa_K590090/annotation2160578/range2160578/1
component: BBa_K314103

https://synbiohub.org/public/igem/BBa_K590090/annotation2160578/range2160578/1
type: Range
persistent identity: range2160578
display id: range2160578
version: 1
top level: BBa_K590090
direction: direction/reverse
start: 1
end: 1638
orientation: inline

BBa_K590059
type: SequenceAnnotation
persistent identity: annotation2160581
display id: annotation2160581
version: 1
top level: BBa_K590090
location: https://synbiohub.org/public/igem/BBa_K590090/annotation2160581/range2160581/1
component: BBa_K590059

https://synbiohub.org/public/igem/BBa_K590090/annotation2160581/range2160581/1
type: Range
persistent identity: range2160581
display id: range2160581
version: 1
top level: BBa_K590090
direction: direction/reverse
start: 1647
end: 3108
orientation: inline

BBa_K590060
type: SequenceAnnotation
persistent identity: annotation2160584
display id: annotation2160584
version: 1
top level: BBa_K590090
location: https://synbiohub.org/public/igem/BBa_K590090/annotation2160584/range2160584/1
component: BBa_K590060

https://synbiohub.org/public/igem/BBa_K590090/annotation2160584/range2160584/1
type: Range
persistent identity: range2160584
display id: range2160584
version: 1
top level: BBa_K590090
direction: direction/reverse
start: 3117
end: 4062
orientation: inline

BBa_K590061
type: SequenceAnnotation
persistent identity: annotation2160587
display id: annotation2160587
version: 1
top level: BBa_K590090
location: https://synbiohub.org/public/igem/BBa_K590090/annotation2160587/range2160587/1
component: BBa_K590061

https://synbiohub.org/public/igem/BBa_K590090/annotation2160587/range2160587/1
type: Range
persistent identity: range2160587
display id: range2160587
version: 1
top level: BBa_K590090
direction: direction/reverse
start: 4071
end: 5229
orientation: inline

BBa_K314103
type: ComponentDefinition
persistent identity: BBa K314103
display id: BBa_K314103
version: 1
was generated by: iGEM to SBOL conversion
description: Lac induced expression cassette
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: construct
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lac induced protein expression insert includes f1 origin, Lac I gene and RBS
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
star: false
creator: Tomas Huber, Justin Siegel
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K314111
component: BBa_K314110
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_K314110
sequence annotation: BBa_K314111
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K314103_sequence/1

BBa_K314111
type: Component
persistent identity: component2091082
display id: component2091082
version: 1
top level: BBa_K314103
definition: BBa_K314111
access: public

BBa_K314110
type: Component
persistent identity: component2091081
display id: component2091081
version: 1
top level: BBa_K314103
definition: f1 ori
access: public

BBa_B0034
type: Component
persistent identity: component2091089
display id: component2091089
version: 1
top level: BBa_K314103
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2091083
display id: component2091083
version: 1
top level: BBa_K314103
definition: lacI+pL
access: public

BBa_K314110
type: SequenceAnnotation
persistent identity: annotation2091081
display id: annotation2091081
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091081/range2091081/1
component: BBa_K314110

https://synbiohub.org/public/igem/BBa_K314103/annotation2091081/range2091081/1
type: Range
persistent identity: range2091081
display id: range2091081
version: 1
top level: BBa_K314103
direction: direction/reverse
start: 1
end: 455
orientation: inline

BBa_K314111
type: SequenceAnnotation
persistent identity: annotation2091082
display id: annotation2091082
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091082/range2091082/1
component: BBa_K314111

https://synbiohub.org/public/igem/BBa_K314103/annotation2091082/range2091082/1
type: Range
persistent identity: range2091082
display id: range2091082
version: 1
top level: BBa_K314103
direction: direction/reverse
start: 464
end: 1555
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2091083
display id: annotation2091083
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091083/range2091083/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K314103/annotation2091083/range2091083/1
type: Range
persistent identity: range2091083
display id: range2091083
version: 1
top level: BBa_K314103
direction: direction/forward
start: 1564
end: 1617
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2091089
display id: annotation2091089
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091089/range2091089/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K314103/annotation2091089/range2091089/1
type: Range
persistent identity: range2091089
display id: range2091089
version: 1
top level: BBa_K314103
direction: direction/reverse
start: 1627
end: 1638
orientation: inline

LuxC
type: ComponentDefinition
persistent identity: BBa K590059
display id: BBa_K590059
version: 1
was generated by: iGEM to SBOL conversion
description: LuxC; standalone, E. Coli codon optimized
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
mutable description: A synthesized version of the LuxC gene from the Cambridge 2010 LuxBrick
discontinued: false
dominant: false
experience: experience/Issues
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBD
star: false
creator: Chris Choe and David Zong
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: LuxC
sequence: https://synbiohub.org/public/igem/BBa_K590059_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129313
display id: annotation2129313
version: 1
top level: LuxC
location: https://synbiohub.org/public/igem/BBa_K590059/annotation2129313/range2129313/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K590059/annotation2129313/range2129313/1
type: Range
persistent identity: range2129313
display id: range2129313
version: 1
top level: LuxC
direction: direction/forward
start: 1
end: 12
orientation: inline

LuxC
type: SequenceAnnotation
persistent identity: annotation2129314
display id: annotation2129314
version: 1
top level: LuxC
location: https://synbiohub.org/public/igem/BBa_K590059/annotation2129314/range2129314/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K590059/annotation2129314/range2129314/1
type: Range
persistent identity: range2129314
display id: range2129314
version: 1
top level: LuxC
direction: direction/forward
start: 19
end: 1462
orientation: inline

LuxD
type: ComponentDefinition
persistent identity: BBa K590060
display id: BBa_K590060
version: 1
was generated by: iGEM to SBOL conversion
description: LuxD; standalone, E. Coli codon optimized
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
mutable description: A synthesized version of the LuxD gene from the Cambridge 2010 LuxBrick
discontinued: false
dominant: false
experience: experience/Issues
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBD
star: false
creator: Chris Choe and David Zong
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: LuxD
sequence: https://synbiohub.org/public/igem/BBa_K590060_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129315
display id: annotation2129315
version: 1
top level: LuxD
location: https://synbiohub.org/public/igem/BBa_K590060/annotation2129315/range2129315/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K590060/annotation2129315/range2129315/1
type: Range
persistent identity: range2129315
display id: range2129315
version: 1
top level: LuxD
direction: direction/forward
start: 1
end: 12
orientation: inline

LuxD
type: SequenceAnnotation
persistent identity: annotation2129316
display id: annotation2129316
version: 1
top level: LuxD
location: https://synbiohub.org/public/igem/BBa_K590060/annotation2129316/range2129316/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K590060/annotation2129316/range2129316/1
type: Range
persistent identity: range2129316
display id: range2129316
version: 1
top level: LuxD
direction: direction/forward
start: 19
end: 946
orientation: inline

LuxE
type: ComponentDefinition
persistent identity: BBa K590061
display id: BBa_K590061
version: 1
was generated by: iGEM to SBOL conversion
description: LuxE; standalone, E. Coli codon optimized
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Gene-synthesis
owned by: james
owned by: myers
mutable description: A synthesized version of the LuxE gene from the Cambridge 2010 LuxBrick
discontinued: false
dominant: false
experience: experience/Issues
group u list: _761_
m user id: 0
owner id: 10712
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBD
star: false
creator: Chris Choe and David Zong
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: LuxE
sequence: https://synbiohub.org/public/igem/BBa_K590061_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2129317
display id: annotation2129317
version: 1
top level: LuxE
location: https://synbiohub.org/public/igem/BBa_K590061/annotation2129317/range2129317/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K590061/annotation2129317/range2129317/1
type: Range
persistent identity: range2129317
display id: range2129317
version: 1
top level: LuxE
direction: direction/forward
start: 1
end: 12
orientation: inline

LuxE
type: SequenceAnnotation
persistent identity: annotation2129318
display id: annotation2129318
version: 1
top level: LuxE
location: https://synbiohub.org/public/igem/BBa_K590061/annotation2129318/range2129318/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K590061/annotation2129318/range2129318/1
type: Range
persistent identity: range2129318
display id: range2129318
version: 1
top level: LuxE
direction: direction/forward
start: 19
end: 1159
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

f1 ori
type: ComponentDefinition
persistent identity: BBa K314110
display id: BBa_K314110
version: 1
was generated by: iGEM to SBOL conversion
description: f1 origin
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: Pet 29 vector
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
star: false
creator: IGEM 2010 Team Washington
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: f1 origin
sequence: https://synbiohub.org/public/igem/BBa_K314110_sequence/1

f1 origin
type: SequenceAnnotation
persistent identity: annotation2115768
display id: annotation2115768
version: 1
top level: f1 ori
location: https://synbiohub.org/public/igem/BBa_K314110/annotation2115768/range2115768/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K314110/annotation2115768/range2115768/1
type: Range
persistent identity: range2115768
display id: range2115768
version: 1
top level: f1 ori
direction: direction/forward
start: 18
end: 324
orientation: inline

BBa_K314111
type: ComponentDefinition
persistent identity: BBa K314111
display id: BBa_K314111
version: 1
was generated by: iGEM to SBOL conversion
description: Lac I
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: Pet 29 vector
owned by: james
owned by: myers
mutable description: Lac I gene with promoter and RBS
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: IGEM 2010 Team Washington
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K314111_sequence/1

https://synbiohub.org/public/igem/BBa_K590090_sequence/1
type: Sequence
persistent identity: BBa K590090 sequence
display id: BBa_K590090_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagagaaagaggagaaatactagatgaacaaatgcatcccgatgattatcaacggtatgatccaggacttcgacaactatgcgtacaaagaagttaagctgaataatgacaaccgtgtcaaactctctgtgattaccgaatcttctgtttctaaaaccctgaacatcaaggaccgtatcaacctcaacctgaatcagattgttaactttctgtacacggtaggccagcgttggaaatctgaagaatacaatcgtcgtcgtacctacatccgcgaactgaaaacctacctgggttactccgacgaaatggcccgtctcgaagccaactggattgcgatgctgctctgctctaaatctgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314103_sequence/1
type: Sequence
persistent identity: BBa K314103 sequence
display id: BBa_K314103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K590059_sequence/1
type: Sequence
persistent identity: BBa K590059 sequence
display id: BBa_K590059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaacaaatgcatcccgatgattatcaacggtatgatccaggacttcgacaactatgcgtacaaagaagttaagctgaataatgacaaccgtgtcaaactctctgtgattaccgaatcttctgtttctaaaaccctgaacatcaaggaccgtatcaacctcaacctgaatcagattgttaactttctgtacacggtaggccagcgttggaaatctgaagaatacaatcgtcgtcgtacctacatccgcgaactgaaaacctacctgggttactccgacgaaatggcccgtctcgaagccaactggattgcgatgctgctctgctctaaatctgcgctgtacgacatcgttaactacgacctgggctctatccacgttctggatgagtggctcccgcgtggcgactgctacgttaaagcgcagccaaaaggcgtttctgttcacctgctggcgggtaatgttccgctctccggtgttacctctattctgcgtgcgatcctcaccaagaacgagtgcatcattaaaacctcttcttctgatccattcacggcgaacgcgctggtttcctcctttatcgacgtcaacgccgaccacccgatcacgaagtctatgtctgttatgtactggcctcacgacgaggacatgacgctctctcagcgtatcatgaaccacgccgatgtggttatcgcgtggggtggtgacgaagcgatcaaatgggcggttaaatactctccgccgcacgttgacatcctgaaattcggtccaaaaaagtccctgtctatcattgaggcgccgaaggacatcgaagcagccgcaatgggtgtggcgcatgacatctgcttctacgatcagcaggcgtgcttctctacccaggacgtttactacatcggtgataacctgccactgttcctgaacgaactcgaaaaacagctggaccgctacgcgaaaatcctccctaaaggctctaacagctttgacgaaaaagcggcgttcactctgaccgagaaggagtctctgttcgcaggctatgaagttcgtaaaggtgacaaacaagcctggctgattgttgtttctccgaccaactcttttggtaaccagccgctgagccgttctgtctacgttcaccaggtttctgacatcaaagaaatcatcccgttcgttaataagaaccgtacgcaaaccgtttctatctacccgtgggaagcgtctctgaaataccgtgacaagctcgcgcgcagcggtgtcgaacgtatcgttgagtctggtatgaataacatcttccgtgttggtggtgcacacgattctctgtctccgctccagtatctggttcgtttcgtgtctcacgaacgtccgttcaactacaccacgaaagacgttgcggttgaaatcgagcagacccgttatctggaagaggacaaattcctggttttcgttccgtaataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K590060_sequence/1
type: Sequence
persistent identity: BBa K590060 sequence
display id: BBa_K590060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaagacgaatctgcgttcttcactattgaccacattatcaagctggacaacggccagtctatccgtgtttgggaaaccctgccaaagaagaatgtacctgaaaaaaagcacaccatcctcattgcttctggtttcgcgcgtcgtatggaccacttcgcgggtctggcagaatacctgtctaccaacggtttccacgttattcgttacgactctctgcatcacgttggtctcagctctggctgtatcaacgagttcaccatgtctatcggtaaaaacagcctgctcactgttgttgactggctgaccgaccacggtgttgaacgtatcggcctgattgcggcgtctctctccgcgcgtattgcgtacgaagtcgtcaataagatcaaactcagcttcctgatcactgcggttggtgttgtaaacctgcgtgacacgctggagaaggcactggaatacgactatctgcaactgccgatctctgagctgccggaggatctcgatttcgaaggccacaacctcggttctgaggtttttgttaccgactgcttcaaacacaactgggacaccctggactctaccctcaactctgtcaaaggtctcgctatcccgttcatcgcgtttaccgcgaacgatgattcttgggttaaacagtctgaagttatcgaactgatcgactctatcgaatcttctaactgcaaactgtactccctgatcggttcctctcacgatctgggtgaaaacctggttgtcctgcgtaacttctaccagtctgttaccaaagcggcgctggcgctcgacgacggtctgctggacctggaaattgacatcatcgaaccgcgtttcgaggacgttacctctatcaccgttaaagaacgtcgtctcaaaaacgagattgagaacgaactcctggaactggcgtaataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K590061_sequence/1
type: Sequence
persistent identity: BBa K590061 sequence
display id: BBa_K590061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccgttcacaccgaatacaaacgtaatcaaatcatcgcatcttctgaaattgatgacctgatcttcatgaccaaacctcaggaatggtcctttgaagagcagaaggagatccgcgataagctcgtacgtgaagcgttctacttccactacaaccgtaatgaggaataccgtaactactgcatcaaccagcacgtttctgacaacctgcacacgatcgacgaaatcccggttttcccgacctctgtgtttaagtataagaaactccacacggtaaccgctgaagacattgaaaactggtacacctcttctggtactcgtggtgtcaagtctcacatcgcgcgtgatcgcctgtccatcgaacgtctgctgggttctgttaacttcggtatgaagtacgttggtgactggttcgagcaccaaatggaactgatcaacctcggcccagaccgctttaataccaacaatatttggttcaaatatgttatgtctctcgttgagctgctgtacccgacggagtttactgtcgataatgataagattgacttcgaaaagaccgttaagcacctgttccgtatcaaaaactctaaaaaggacatttgtctgatcggtccgccgttcttcgtttacctcctgtgccagtacatgaaagagaataacatcgagttcaagggtggtgaccgtgttcacatcatcaccggcggtggttggaaatctaaccaaaacgactctctggaccgtgccgacttcaaccaactcctgatggatacctttcagctcgacaagattaaccagattcgcgacacctttaatcaggttgaactgaatacctgctttttcgaggacgagttccagcgtaaacacgttccgccttgggtttatgcacgcgccctggacccggaaaccctgaaaccagttgcggacggcgaaatcggcctgctctcttacatggatgcgtcttctaccgcgtacccggcgttcatcgttaccgacgacatcggtattgtgaaagaaatccgtgaaccggacccgtaccctggcgtcactgttgaaatcgttcgtcgcctgaacacccgcgcacagaaaggttgcgcgctgtctatggcgaacgtgatccagaaaaacatcaaagactaataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314110_sequence/1
type: Sequence
persistent identity: BBa K314110 sequence
display id: BBa_K314110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314111_sequence/1
type: Sequence
persistent identity: BBa K314111 sequence
display id: BBa_K314111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z