LOCUS       BBa_K592014             1655 bp    DNA     linear       21-May-2024
DEFINITION  HO1-pcyA, low expression, B1001 terminator
ACCESSION   BBa_K592014
VERSION     BBa_K592014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    788..812
                     /label=Help:Barcodes
     CDS             62..812
                     /label=BBa_I15008
     misc_feature    825..828
                     /label=conserved
     RBS             821..832
                     /label=BBa_B0034
     CDS             839..1588
                     /label=PcyA
     misc_feature    1589..1613
                     /label=Help:Barcodes
     CDS             839..1613
                     /label=BBa_I15009
     stem_loop       1631..1646
                     /label=stem loop
     polyA_site      1647..1655
                     /label=Poly A tail
     polyA_site      1622..1630
                     /label=PolyA tail
     misc_feature    1622..1655
                     /label=B1001
     terminator      1622..1655
                     /label=BBa_B1001
     misc_feature    44..1655
                     /label=BBa_K592013
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta
       61 gatgagtgtc aacttagctt cccagttgcg ggaagggacg aaaaaatccc actccatggc
      121 ggagaacgtc ggctttgtca aatgcttcct caagggcgtt gtcgagaaaa attcctaccg
      181 taagctggtt ggcaatctct actttgtcta cagtgccatg gaagaggaaa tggcaaaatt
      241 taaggaccat cccatcctca gccacattta cttccccgaa ctcaaccgca aacaaagcct
      301 agagcaagac ctgcaattct attacggctc caactggcgg caagaagtga aaatttctgc
      361 cgctggccaa gcctatgtgg accgagtccg gcaagtggcc gctacggccc ctgaattgtt
      421 ggtggcccat tcctacaccc gttacctggg ggatctttcc ggcggtcaaa ttctcaagaa
      481 aattgcccaa aatgccatga atctccacga tggtggcaca gctttctatg aatttgccga
      541 cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa gctatgaatg atctgcccat
      601 tgaccaagcc accgccgaac ggattgtgga tgaagccaat gacgcctttg ccatgaacat
      661 gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg atcggcatta tggtgttcaa
      721 cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt ggcctcgcca cctccgaagg
      781 ctaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat
      841 ggccgtcact gatttaagtt tgaccaattc ttccctgatg cctacgttga acccgatgat
      901 tcaacagttg gccctggcga tcgccgctag ttggcaaagt ttacccctca agccctatca
      961 attgccggag gatttgggct acgtagaagg ccgcctggaa ggggaaaagt tagtgattga
     1021 aaatcggtgc taccaaacgc cccagtttcg caaaatgcat ttggagttgg ccaaggtggg
     1081 caaagggttg gatattctcc actgtgtaat gtttcctgag cctttatacg gtctaccttt
     1141 gtttggctgt gacattgtgg ccggccccgg tggagtaagt gcggctattg cggatctatc
     1201 ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa aaatcattgg cagagctagg
     1261 ccagccagaa tttgagcaac aacgggaatt gcccccctgg ggagaaatat tttctgaata
     1321 ttgtttattc atccgtccca gcaatgtcac tgaagaagaa agatttgtac aaagggtagt
     1381 ggactttttg caaattcatt gtcaccaatc catcgttgcc gaacccttgt ctgaagctca
     1441 aactttggag caccgtcagg ggcaaattca ttactgccaa caacaacaga aaaatgataa
     1501 aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg gcggaacggt atatgagcca
     1561 agtcttattt gatgttatcc aataataact ctgatagtgc tagtgtagat ctctactaga
     1621 gaaaaaaaaa ccccgcttcg gcggggtttt ttttt
//