BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K592001 1 BBa_K592001 CcaS, green light sensor from Synechocystis sp. PCC6803 2011-09-20T11:00:00Z 2015-05-08T01:12:48Z Synechocystic sp. PCC6803 CcaS is a green light sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by [[Part:BBa_I15008|ho1]] and [[Part:BBa_I15009|PcyA]]. Furthermore, CcaS works in conjunction with [[Part:BBa_K592002|CcaR]], its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ. false false _763_ 0 7929 9 It's complicated false This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. false Lei Sun annotation2136230 1 ccaS range2136230 1 1 2265 BBa_K592002 1 BBa_K592002 ccaR 2011-09-08T11:00:00Z 2015-05-08T01:12:48Z The ccaR was amplified from a pJT118 provided by Voight et al (2011) Multichromatic Control of Gene Expression in Escherichia coli, J. Mol. Biol., 405, p315???324 Released HQ 2013 ccaR is the response regulator of the green sensor that also includes the membrane-associated histidine kinase ccaS. The two-componet system is also dependent of a chromophore. false false _763_ 0 9827 9 In stock false The ccaR amplified from pJT118 was point mutated to get rid of illegal restriction site. false Erik Lundin annotation2136237 1 cds range2136237 1 1 708 BBa_K592017 1 BBa_K592017 B0034-ccaS-B0034-ccaR 2011-09-20T11:00:00Z 2015-05-08T01:12:49Z CcaR and CcaS can be found in the genome of Synechocystis sp. PCC6803. This gene consists of the green light sensing protein CcaS along with its response regulator CcaR. false false _763_ 0 7929 9 It's complicated false The SYnechocystis sp. codon usage not tuned for expression in E.coli. Strong RBS to ensure expression. false Lei Sun component2136349 1 BBa_K592002 component2136345 1 BBa_K592001 component2136347 1 BBa_B0034 component2136343 1 BBa_B0034 annotation2136345 1 BBa_K592001 range2136345 1 19 2283 annotation2136349 1 BBa_K592002 range2136349 1 2310 3017 annotation2136347 1 BBa_B0034 range2136347 1 2292 2303 annotation2136343 1 BBa_B0034 range2136343 1 1 12 BBa_B0034_sequence 1 aaagaggagaaa BBa_K592001_sequence 1 atgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccggtccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttgaagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgtcagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgctaaaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttaataa BBa_K592017_sequence 1 aaagaggagaaatactagatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccggtccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttgaagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgtcagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgctaaaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttaataatactagagaaagaggagaaatactagatgcgcattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactaataa BBa_K592002_sequence 1 atgcgcattcttttagtggaggatgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z