LOCUS       BBa_K592021             1767 bp    DNA     linear       22-Nov-2024
DEFINITION  PompC-tetR inverter-B0034-mCherry
ACCESSION   BBa_K592021
VERSION     BBa_K592021.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             138..754
                     /label=tetR
     misc_feature    755..788
                     /label=SsrA
     misc_feature    795..819
                     /label=Help:Barcodes
     CDS             135..794
                     /label=BBa_C0040
     stem_loop       839..882
                     /label=stem_loop
     misc_feature    828..907
                     /label=BBa_B0010
     terminator      828..907
                     /label=BBa_B0010
     stem_loop       923..942
                     /label=T7 TE
     misc_feature    949..949
                     /label=stop
     polyA_site      943..956
                     /label=polya
     misc_feature    916..956
                     /label=BBa_B0012
     terminator      916..956
                     /label=BBa_B0012
     misc_feature    965..983
                     /label=TetR 1
     misc_feature    965..1018
                     /label=BBa_R0040
     promoter        984..989
                     /label=-35
     misc_feature    990..1008
                     /label=TetR 2
     promoter        1007..1012
                     /label=-10
     promoter        965..1018
                     /label=BBa_R0040
     misc_feature    117..1018
                     /label=BBa_Q04400
     misc_feature    1031..1034
                     /label=conserved
     RBS             1027..1038
                     /label=BBa_B0034
     CDS             1045..1752
                     /label=mCherry
     misc_feature    1396..1396
                     /label=C->T (removing PstI site)
     misc_feature    1753..1761
                     /label=LVA
     misc_feature    1045..1767
                     /label=BBa_J06505
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc
      181 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
      241 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
      301 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
      361 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
      421 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
      481 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
      541 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
      601 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
      661 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
      721 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt
      781 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
      841 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      901 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      961 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
     1021 ctagagaaag aggagaaata ctagatggtg agcaagggcg aggaggataa catggccatc
     1081 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1141 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1201 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1261 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1321 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1381 acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1441 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1501 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1561 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1621 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1681 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1741 gagctgtaca agttagtagc ttaataa
//