LOCUS       BBa_K592027              858 bp    DNA     linear       22-Nov-2024
DEFINITION  T5-lac promoter-B0032-BFP
ACCESSION   BBa_K592027
VERSION     BBa_K592027.1  
FEATURES             Location/Qualifiers
     promoter        1..126
                     /label=T5-lac promoter
     misc_feature    1..26
                     /label=lacO1 site
     misc_feature    69..86
                     /label=lacO1 site
     misc_feature    94..123
                     /label=lacOi site
     promoter        63..68
                     /label=-35 signal
     promoter        87..93
                     /label=-10 signal
     promoter        1..126
                     /label=BBa_K592008
     RBS             135..147
                     /label=RBS-3\Weak
     RBS             141..144
                     /label=RBS
     misc_feature    135..147
                     /label=BBa_B0032
     RBS             135..147
                     /label=BBa_B0032
     CDS             154..852
                     /label=mTagBFP
     CDS             154..858
                     /label=BBa_K592100
ORIGIN
        1 tgtggaattg tgagcggata acaattacga gcttcatgca cagtgaaatc atgaaaaatt
       61 tatttgcttt gtgagcggat aacaattata atatgtggaa ttgtgagcgc tcacaattcc
      121 acaacgtact agagtcacac aggaaagtac tagatgagcg aactgatcaa agagaacatg
      181 cacatgaagc tgtacatgga aggcaccgtt gacaaccacc actttaagtg cacgtctgag
      241 ggtgagggta agccgtacga aggcacccaa accatgcgta tcaaagttgt ggagggcggt
      301 ccactgccgt tcgcttttga cattctggcg accagcttcc tgtacggttc caaaacgttc
      361 attaaccata ctcagggcat tccggatttc ttcaaacaga gctttccgga aggtttcacc
      421 tgggagcgtg tcaccacgta tgaagatggt ggtgtgttga ccgccaccca agatacctcc
      481 ctgcaagatg gctgtctgat ctataacgtg aaaattcgtg gcgtcaactt tacgagcaat
      541 ggtccggtga tgcagaagaa aaccctgggt tgggaggcgt ttacggaaac cctgtatccg
      601 gccgatggtg gcctggaggg ccgtaacgac atggcactga agctggttgg tggcagccat
      661 ttgatcgcaa atatcaagac gacgtaccgc agcaagaaac cggcgaaaaa tctgaagatg
      721 ccgggtgttt actatgtcga ctaccgtctg gaacgcatta aagaagcgaa taatgagact
      781 tacgtggagc agcacgaggt tgcagtcgcg cgctattgcg acttgcctag caagctgggt
      841 cataaactga attaataa
//