BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_K592027
1
BBa_K592027
T5-lac promoter-B0032-BFP
2011-09-20T11:00:00Z
2015-05-08T01:12:49Z
The information of <partinfo>BBa_K592008</partinfo> and <partinfo>BBa_K592100</partinfo> can be found in the registry. T5-lac promoter is a fusion promoter with parts from T5 phage.
Released HQ 2013
The promoter T5-lac (<partinfo>BBa_K592008</partinfo>) for testing its promoter strength coupled with BFP (<partinfo>BBa_K592100</partinfo>). This construct should be cloned into pSB3K3 plasmid to measure the BFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.
false
false
_763_
0
7929
9
In stock
false
This part was assembled into pSB3K3 for testing, and later cloned into pSB1C3 for submission.
false
Lei Sun
component2136786
1
BBa_K592100
component2136783
1
BBa_B0032
component2136781
1
BBa_K592008
annotation2136786
1
BBa_K592100
range2136786
1
154
858
annotation2136781
1
BBa_K592008
range2136781
1
1
126
annotation2136783
1
BBa_B0032
range2136783
1
135
147
BBa_K592100
1
BFP
Blue Fluorescent Protein (mTagBFP)
2011-09-19T11:00:00Z
2016-01-25T04:45:27Z
Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
Released HQ 2013
This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
false
_763_
4206
10137
9
In stock
true
The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
Erik Gullberg
annotation2135490
1
mTagBFP
range2135490
1
1
699
BBa_K592008
1
PT5lac
T5-lac Promoter
2011-09-10T11:00:00Z
2015-05-08T01:12:48Z
Phage T5
Released HQ 2013
T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.
false
false
_763_
0
7929
9
In stock
false
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
false
Erik Lundin
annotation2135661
1
-10 signal
range2135661
1
87
93
annotation2135656
1
lacO1 site
range2135656
1
1
26
annotation2135658
1
lacOi site
range2135658
1
94
123
annotation2135659
1
-35 signal
range2135659
1
63
68
annotation2135657
1
lacO1 site
range2135657
1
69
86
annotation2127135
1
T5-lac promoter
range2127135
1
1
126
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K592027_sequence
1
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagtcacacaggaaagtactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataa
BBa_K592100_sequence
1
atgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataa
BBa_K592008_sequence
1
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z