BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K592033
1
BBa_K592033
PcpcG2-B0034
2011-09-20T11:00:00Z
2015-05-08T01:12:49Z
PcpcG2: Synechocystis sp. PCC6803.
This is the output promoter to the CcaS/CcaR green light-sensing system with RBS. One can add any coding gene downstream to it in order to introduce control by green light.
false
false
_763_
0
7929
9
Not in stock
false
This construct is made to make it easy for users to just add any gene to the green light-sensing output.
false
Lei Sun
component2137385
1
BBa_K592003
component2137387
1
BBa_B0034
annotation2137385
1
BBa_K592003
range2137385
1
1
238
annotation2137387
1
BBa_B0034
range2137387
1
247
258
BBa_K592003
1
BBa_K592003
PcpcG2 promoter, ccaR-regulated
2011-09-16T11:00:00Z
2015-05-08T01:12:48Z
This sequence can be amplified from the genome of Synechocystis PCC6803.
Reference: Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M (2008)
Cyanobacteriochrome CcaS is the green light receptor that induces the expression of
phycobilisome linker protein. Proc Natl Acad Sci U S A 105: 9528-9533.
The 238 bp sequence can be directly taken from the pJT122 plasmid designed by Jeffrey Tabor.
Reference: Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene
expression in Escherichia coli. J. Mol. Biol. 405, 2, 315???324.
PcpcG2 is the green-light activated promoter from the genome of Synechocystis PCC6803. This 238 bp sequence is used by Jeffrey Tabor in pJT122 plasmid and contains the entire region upstream of cpcG2 and downstream of ccaR.
PcpcG2 is regulated by the light-activation of ccaS/ccaR. The gene downstream to this promoter is transcribed upon activation by ccaS/ccaR, via green light.
false
false
_763_
0
7929
9
Not in stock
false
This sequence was PCR-amplified directly from the pJT122 plasmid provided by Chris Voigt, the co-author of Tabor's paper on multichromatic light-sensing (Tabor et al. 2011). Due to its nature, optimizing primers to extract this sequence from the source plasmid was difficult. Recommend touchdown-PCR.
false
Lei Sun
annotation2130109
1
PcpcG2 promoter
range2130109
1
1
238
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K592033_sequence
1
agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatcttactagagaaagaggagaaa
BBa_K592003_sequence
1
agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z