LOCUS       BBa_K592200             3137 bp    DNA     linear       22-Dec-2024
DEFINITION  Low to medium copy BioBrick standard vector
ACCESSION   BBa_K592200
VERSION     BBa_K592200.1  
FEATURES             Location/Qualifiers
     misc_feature    35..46
                     /label=Translational stop BBa_B0042
     misc_feature    47..115
                     /label=Terminator BBa_B0054
     primer_bind     116..135
                     /label=VR site BBa_G00102
     misc_feature    136..176
                     /label=Terminator BBa_B0062
     misc_feature    184..1748
                     /label=p15A low to medium copy replication origin 
                     BBa_I50032
     primer_bind     1726..1745
                     /label=Lambda red F primer
     CDS             1841..2840
                     /label=Spectinomycin resistance marker
     promoter        2907..2933
                     /label=SpcR promoter
     misc_feature    2952..2992
                     /label=P22 late terminator
     primer_bind     2993..3012
                     /label=VF2 site BBa_G00100
     misc_feature    3013..3090
                     /label=T3 terminator BBa_B0055
     misc_feature    3091..3102
                     /label=Translational stop BBa_B0042
     primer_bind     20..41
                     /label=Lambda Red R primer
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt
      601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
      661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
      721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
      781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
     1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
     1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
     1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac
     1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct
     1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac
     1741 tagaggcgtc gaccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     1801 taagggcacc aataactgcc ttaaaaaaat tatttgccga ctaccttggt gatctcgcct
     1861 ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
     1921 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
     1981 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
     2041 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
     2101 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
     2161 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     2221 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
     2281 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     2341 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
     2401 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     2461 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
     2521 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     2581 gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta
     2641 gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     2701 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca
     2761 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     2821 gaccagttgc gtaagcgcat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     2881 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     2941 tcaacgagct caaataaagc cctgagttta accgctcggg gctttttgcg tttgccacct
     3001 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
     3061 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
     3121 cgcggccgct tctagag
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