##gff-version 3
##sequence-region BBa_K592200 20 3102
BBa_K592200	.	sequence_feature	3013	3090	.	+	0	ID=annotation2136379;Name=T3 terminator BBa_B0055
BBa_K592200	.	primer_binding_site	2993	3012	.	+	0	ID=annotation2136378;Name=VF2 site BBa_G00100
BBa_K592200	.	stop_codon	3091	3102	.	+	0	ID=annotation2136380;Name=Translational stop BBa_B0042
BBa_K592200	.	sequence_feature	47	115	.	+	0	ID=annotation2136370;Name=Terminator BBa_B0054
BBa_K592200	.	CDS	1841	2840	.	+	0	ID=annotation2136375;Name=Spectinomycin resistance marker
BBa_K592200	.	promoter	2907	2933	.	+	0	ID=annotation2136376;Name=SpcR promoter
BBa_K592200	.	primer_binding_site	116	135	.	+	0	ID=annotation2136371;Name=VR site BBa_G00102
BBa_K592200	.	sequence_feature	184	1748	.	+	0	ID=annotation2136373;Name=p15A low to medium copy replication origin BBa_I50032
BBa_K592200	.	sequence_feature	136	176	.	+	0	ID=annotation2136372;Name=Terminator BBa_B0062
BBa_K592200	.	sequence_feature	2952	2992	.	+	0	ID=annotation2136377;Name=P22 late terminator
BBa_K592200	.	primer_binding_site	20	41	.	+	0	ID=annotation2136398;Name=Lambda Red R primer
BBa_K592200	.	stop_codon	35	46	.	+	0	ID=annotation2136369;Name=Translational stop BBa_B0042
BBa_K592200	.	primer_binding_site	1726	1745	.	+	0	ID=annotation2136374;Name=Lambda red F primer
>BBa_K592200
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcgg
cccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggg
gccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacag
atcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatt
taaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtga
gttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgc
tctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaact
ggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctc
taaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagt
gtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgc
acgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagat
ttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatct
tcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcga
gtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgcca
catgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcct
cgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttga
tgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaag
atgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgt
tacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttt
actagaggcgtcgaccattcatccgcttattatcacttattcaggcgtagcaaccaggcgtttaagggcaccaataact
gccttaaaaaaattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcg
cgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggca
ggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaa
cgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctca
aatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgctt
ttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattc
tccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcg
cggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaa
gccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatg
tacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatc
accgcttccctcatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctccataacatca
aacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtaccccaaaaaaacagtcataaca
agccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtaagcgcatttta
gcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttgg
aacctcttacgtgccgatcaacgagctcaaataaagccctgagtttaaccgctcggggctttttgcgtttgccacctga
cgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgatt
gctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagag