LOCUS       BBa_K592201             2967 bp    DNA     linear       01-Jun-2024
DEFINITION  Low to medium copy Lambda Red recombineering compatible plasmid
ACCESSION   BBa_K592201
VERSION     BBa_K592201.1  
FEATURES             Location/Qualifiers
     primer_bind     20..41
                     /label=Lambda Red R primer
     misc_feature    35..46
                     /label=Translational stop BBa_B0042
     misc_feature    47..115
                     /label=Terminator BBa_B0054
     primer_bind     116..135
                     /label=VR site BBa_G00102
     misc_feature    136..176
                     /label=Terminator BBa_B0062
     misc_feature    184..1748
                     /label=p15A ori BBa_I50032
     primer_bind     1726..1745
                     /label=L-red_F_primer 
     misc_feature    1785..1826
                     /label=FRT site
     CDS             2035..2673
                     /label=Chloramphenicol resistant marker
     misc_feature    2723..2756
                     /label=FRT site
     misc_feature    2782..2822
                     /label=P22 late terminator
     primer_bind     2823..2842
                     /label=VF2 site BBa_G00100
     misc_feature    2843..2920
                     /label=T3 terminator BBa_B0055
     misc_feature    2921..2932
                     /label=translational stop BBa_B0042
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt
      601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
      661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
      721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
      781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
     1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
     1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
     1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac
     1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct
     1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac
     1741 tagaggcgtc gacgccatgg tccatatgaa tatcctcctt agttcctatt ccgaagttcc
     1801 tattctctag aaagtatagg aacttcggcg cgcctacctg tgacggaaga tcacttcgca
     1861 gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga
     1921 aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
     1981 ctaccgggcg tattttttga gttgtcgaga ttttcaggag ctaaggaagc taaaatggag
     2041 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     2101 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     2161 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     2221 cttgcccgcc tgatgaatgc tcatccggaa ttacgtatgg caatgaaaga cggtgagctg
     2281 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     2341 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     2401 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     2461 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     2521 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     2581 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     2641 agatgcttaa tgaatacaac agtactgcga tgagtggcag ggcggggcgt aaggcgcgcc
     2701 atttaaatga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaag
     2761 cagctccagc ctacagagct caaataaagc cctgagttta accgctcggg gctttttgcg
     2821 tttgccacct gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc
     2881 ccacccgtga aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt
     2941 gactcgaatt cgcggccgct tctagag
//