LOCUS       BBa_K592202             3430 bp    DNA     linear       22-Nov-2024
DEFINITION  Low to medium copy Lambda Red recombineering compatible plasmid
ACCESSION   BBa_K592202
VERSION     BBa_K592202.1  
FEATURES             Location/Qualifiers
     primer_bind     20..41
                     /label=L-red_R_primer 
     misc_feature    35..46
                     /label=Translational stop BBa_B0042
     misc_feature    47..115
                     /label=Terminator BBa_B0054
     primer_bind     116..135
                     /label=VR site BBa_G00102
     misc_feature    136..176
                     /label=Terminator BBa_B0062
     misc_feature    184..1748
                     /label=p15A ori BBa_I50032
     primer_bind     1726..1745
                     /label=L-red_F_primer
     misc_feature    1781..1826
                     /label=FRT site
     CDS             2017..2811
                     /label=Kanamycin resistance marker
     misc_feature    3172..3219
                     /label=FRT site
     misc_feature    3245..3285
                     /label=P22 late terminator
     primer_bind     3286..3305
                     /label=VF2 site BBa_G00100
     misc_feature    3306..3383
                     /label=T3 terminator BBa_B0055
     misc_feature    3384..3395
                     /label=Translational stop BBa_B0042
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt
      601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
      661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
      721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
      781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
     1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
     1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
     1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac
     1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct
     1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac
     1741 tagaggcgtc gacgccatgg tccatatgaa tatcctcctt agttcctatt ccgaagttcc
     1801 tattctctag aaagtatagg aacttcagag cgcttttgaa gctggggtgg gcgaagaact
     1861 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     1921 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     1981 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     2041 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     2101 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     2161 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     2221 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     2281 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     2341 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     2401 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     2461 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     2521 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     2581 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     2641 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     2701 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     2761 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     2821 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     2881 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     2941 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     3001 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     3061 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     3121 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaggggatct tgaagttcct
     3181 attccgaagt tcctattctc tagaaagtat aggaacttcg aagcagctcc agcctacaga
     3241 gctcaaataa agccctgagt ttaaccgctc ggggcttttt gcgtttgcca cctgacgtct
     3301 aagaaaagga atattcagca atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag
     3361 ccagtgagtt gattgctacg taattagtta gttagccctt agtgactcga attcgcggcc
     3421 gcttctagag
//