LOCUS BBa_K593001 2615 bp DNA linear 14-Nov-2024 DEFINITION ROSE regulated kill switch ACCESSION BBa_K593001 VERSION BBa_K593001.1 FEATURES Location/Qualifiers RBS 90..95 /label=SD misc_feature 74..79 /label=Biobrick-Scar-adaptation stem_loop 1..15 /label=Predicted Stem Loop stem_loop 16..36 /label=Predicted Stem Loop stem_loop 39..71 /label=Predicted Stem Loop stem_loop 72..96 /label=Predicted Stem Loop extending to startcodon misc_feature 1..96 /label=BBa_K115001 CDS 103..822 /label=GFP protein CDS 103..822 /label=BBa_E0040 misc_feature 1..822 /label=BBa_S04561 CDS 847..1503 /label=T4 holin CDS 831..1506 /label=BBa_K112805 CDS 1531..2025 /label=T4 Endolysin CDS 1515..2028 /label=BBa_K112806 stem_loop 2048..2091 /label=stem_loop misc_feature 2037..2116 /label=BBa_B0010 terminator 2037..2116 /label=BBa_B0010 stem_loop 2132..2151 /label=T7 TE misc_feature 2158..2158 /label=stop polyA_site 2152..2165 /label=polya misc_feature 2125..2165 /label=BBa_B0012 terminator 2125..2165 /label=BBa_B0012 promoter 2174..2208 /label=BBa_J23116 CDS 2231..2524 /label=T4 antiholin CDS 2217..2527 /label=BBa_K112807 stem_loop 2547..2590 /label=stem_loop misc_feature 2536..2615 /label=BBa_B0010 terminator 2536..2615 /label=BBa_B0010 misc_feature 831..2615 /label=BBa_K112808 misc_feature 1..2615 /label=BBa_S04562 ORIGIN 1 gccgcgacaa gcggtccggg cgccctaggg gcccggcgga gacgggcgcc ggaggtgtcc 61 gacgcctgct cgtcaagttc ttgctccttg gaggattact agatgcgtaa aggagaagaa 121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 301 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 541 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 601 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 781 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag cttaaaagga 841 gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt tggtgttctc 901 gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt agctgtcgta 961 attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt tgagtactat 1021 aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac tgcacgcttt 1081 gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc agactttagt 1141 gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat agcatacgaa 1201 ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt tgataaaact 1261 atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc aaaatttgct 1321 tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg tccatatttt 1381 aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa tgatcatata 1441 agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg aagggctaaa 1501 taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat 1561 gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt 1621 catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt 1681 gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat 1741 gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct 1801 cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt 1861 gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca 1921 gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt 1981 acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta ctagagccag 2041 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2101 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2161 ttatatacta gagttgacag ctagctcagt cctagggact atgctagcta ctagaggata 2221 ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat tgtcatttgt 2281 tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta tggaatctgg 2341 tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg aacaattcta 2401 tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg aatgtataga 2461 gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat tggagactga 2521 atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2581 ttcgttttat ctgttgtttg tcggtgaacg ctctc // LOCUS BBa_S04560 2615 bp DNA linear 14-Nov-2024 DEFINITION S04559:K112808 ACCESSION BBa_S04560 VERSION BBa_S04560.1 FEATURES Location/Qualifiers RBS 90..95 /label=SD misc_feature 74..79 /label=Biobrick-Scar-adaptation stem_loop 1..15 /label=Predicted Stem Loop stem_loop 16..36 /label=Predicted Stem Loop stem_loop 39..71 /label=Predicted Stem Loop stem_loop 72..96 /label=Predicted Stem Loop extending to startcodon misc_feature 1..96 /label=BBa_K115001 CDS 103..822 /label=GFP protein CDS 103..822 /label=BBa_E0040 misc_feature 1..822 /label=BBa_S04559 CDS 847..1503 /label=T4 holin CDS 831..1506 /label=BBa_K112805 CDS 1531..2025 /label=T4 Endolysin CDS 1515..2028 /label=BBa_K112806 stem_loop 2048..2091 /label=stem_loop misc_feature 2037..2116 /label=BBa_B0010 terminator 2037..2116 /label=BBa_B0010 stem_loop 2132..2151 /label=T7 TE misc_feature 2158..2158 /label=stop polyA_site 2152..2165 /label=polya misc_feature 2125..2165 /label=BBa_B0012 terminator 2125..2165 /label=BBa_B0012 promoter 2174..2208 /label=BBa_J23116 CDS 2231..2524 /label=T4 antiholin CDS 2217..2527 /label=BBa_K112807 stem_loop 2547..2590 /label=stem_loop misc_feature 2536..2615 /label=BBa_B0010 terminator 2536..2615 /label=BBa_B0010 misc_feature 831..2615 /label=BBa_K112808 ORIGIN 1 gccgcgacaa gcggtccggg cgccctaggg gcccggcgga gacgggcgcc ggaggtgtcc 61 gacgcctgct cgtcaagttc ttgctccttg gaggattact agatgcgtaa aggagaagaa 121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 301 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 541 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 601 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 781 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag cttaaaagga 841 gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt tggtgttctc 901 gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt agctgtcgta 961 attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt tgagtactat 1021 aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac tgcacgcttt 1081 gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc agactttagt 1141 gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat agcatacgaa 1201 ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt tgataaaact 1261 atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc aaaatttgct 1321 tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg tccatatttt 1381 aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa tgatcatata 1441 agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg aagggctaaa 1501 taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat 1561 gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt 1621 catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt 1681 gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat 1741 gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct 1801 cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt 1861 gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca 1921 gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt 1981 acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta ctagagccag 2041 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2101 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2161 ttatatacta gagttgacag ctagctcagt cctagggact atgctagcta ctagaggata 2221 ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat tgtcatttgt 2281 tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta tggaatctgg 2341 tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg aacaattcta 2401 tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg aatgtataga 2461 gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat tggagactga 2521 atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2581 ttcgttttat ctgttgtttg tcggtgaacg ctctc //