LOCUS       BBa_K593009             2157 bp    DNA     linear       16-Dec-2024
DEFINITION  ROSE regulated kill switch
ACCESSION   BBa_K593009
VERSION     BBa_K593009.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             144..149
                     /label=SD
     misc_feature    128..133
                     /label=Biobrick-Scar-adaptation
     stem_loop       55..69
                     /label=Predicted Stem Loop
     stem_loop       70..90
                     /label=Predicted Stem Loop
     stem_loop       93..125
                     /label=Predicted Stem Loop
     stem_loop       126..150
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    55..150
                     /label=BBa_K115001
     CDS             157..876
                     /label=GFP protein
     CDS             157..876
                     /label=BBa_E0040
     misc_feature    885..2157
                     /label=BBa_K124003
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agaggccgcg
       61 acaagcggtc cgggcgccct aggggcccgg cggagacggg cgccggaggt gtccgacgcc
      121 tgctcgtcaa gttcttgctc cttggaggat tactagatgc gtaaaggaga agaacttttc
      181 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      241 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      301 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
      361 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
      421 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      481 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      541 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
      601 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
      661 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      721 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
      781 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      841 attacacatg gcatggatga actatacaaa taataatact agagaattca tcagtgatag
      901 ttacgttacg ctgcgccttc tttttttccc ttccaatata agaactacgc aatccgttac
      961 tggcggaggc gttgctatga aatcaatgga caaaatctca actggcattg cctacggaac
     1021 atctgctggt agtgcgggat actggttttt gcagtggttg gatcaggtca gtccgtcaca
     1081 gtgggctgcg attggagtgc ttggaagcct tgtgttgggt tttctcactt atctgacaaa
     1141 tctgtatttc aaaatcagag aagacagacg aaaggctgcg agaggtgaat aatgcctcca
     1201 tcattacgaa aagccgttgc tgctgctatt ggtggcggag caattgctat agcatcagtg
     1261 ttaatcactg gcccaagtgg taacgatggt ctggaaggtg tcagctacat accatacaaa
     1321 gatattgttg gtgtatggac tgtatgtcac gggcatacag gaaaagacat catgctcggt
     1381 aaaacgtata ccaaagcaga atgcaaagcc ctcctgaata aagaccttgc cacggtcgcc
     1441 agacaaatta acccgtacat caaagtcgat ataccggaaa caatgcgcgg cgctctttac
     1501 tcattgcttt acaacgtggg tgctggcaat ttcagaacat cgacgcttct tcgtaaaata
     1561 aaccagggcg atatcaaagg cgcatgtgat cagctacgtc gctggacata tgctggcggt
     1621 aagcaatgga aaggtctcat gactcgtcgt gagattgagc gtgaaatctg tttgtggggt
     1681 cagcaatgaa cagagtaacc gcgattatct ccgctctggt tatctgcatc atcgtctgcc
     1741 tgtcatgggc tgttaatcat taccgtgata acgccattac ctacaaagcc cagcgcgaca
     1801 aaaatgccag agaactgacg ctggcgaacc gggtaattac tgacatacag atgcgtcagc
     1861 gtgatgttgc tgcgctcgat gcaaaataca cgaaggagtt agctgatgcg aaagctgaaa
     1921 atgatgctct gcgtgatgat gttgccgctg gtcgtcgtcg gttgcacatc aaagcagtct
     1981 gtcagtcagt gcgtgaagcc acgactgcct ccggcgtgga taatgcagcc tccccccgac
     2041 tggcagacac cgctgaacgg gattatttca ccctcagaga gaggctgatc actatgcaaa
     2101 aacaactgga aggaacccag aagtatatta atgagcagtg cagatagagc tgcccat
//