BBa_K594002 1 leuB subunit of a 3-isopropylmalate dehydrogenase from E.coli BL21 2011-09-29T11:00:00Z 2015-05-08T01:12:49Z This leuB is from the leu operon in BL 21(E.coli),we get BL21 from Takara and found its sequence in NCBI, then cloned it with a Pfu PCR. LeuB is essential to leucine biosynthesis, so it is a necessary gene for E.coli's well growth. The leu operon is responsible for the leucine biosynthesis, including leuA, leuB, leuC, leuD and leuO. Regulation Summary Diagram: Summary: 3-isopropylmalate dehydrogenase (LeuB) carries out the third step in leucine biosynthesis, catalyzing the conversion of 3-isopropylmalate to 2-isopropyl-3-oxosuccinate. 3-isopropylmalate dehydrogenase oxidizes 3-isopropylmalate with NAD+ to generate 2-isopropyl-3-oxosuccinate and NADH. The kMs listed below were determined at 40?? C, as was the kcat value of 69/s. A crystal structure of 3-isopropylmalate dehydrogenase to 2.1 ?? resolution reveals that it is a dimer. false false _765_ 0 8221 9 It's complicated true Besides, when we were looking for leuB gene, we also try to find its RBS, but unluckily, we didn't find its RBS on NCBI, its location is closely linked to leuA's location. false Yu Xiaoyan annotation2147996 1 leuB range2147996 1 1 1092 BBa_K594002_sequence 1 atgtcgaagaattaccatattgccgtattgccgggggacggtattggtccggaagtgatgacccaggcgctgaaagtgctggatgccgtgcgcaaccgctttgcgatgcgcatcaccaccagccattacgatgtaggcggcgcagccattgataaccacgggcaaccactgccgcctgcgacggttgaaggttgtgagcaagccgatgccgtgctgtttggctcggtaggcggcccgaagtgggaacatttaccaccagaccagcaaccagaacgcggcgcgctgctgcctctgcgtaagcacttcaaattattcagcaacctgcgcccggcaaaactgtatcaggggctggaagcattctgtccgctgcgtgcagacattgccgcaaacggcttcgacatcctgtgtgtgcgcgaactgaccggcggcatctatttcggtcagccaaaaggccgcgaaggtagcggacaatatgaaaaagcctttgataccgaggtgtatcaccgttttgagatcgaacgtatcgcccgcatcgcgtttgaatctgctcgcaagcgtcgccacaaagtgacgtcgatcgataaagccaacgtgctgcaatcctctattttatggcgggagatcgttaacgagatcgccacggaatacccggatgtcgaactggcgcatatgtacatcgacaacgccaccatgcagctgattaaagatccatcacagtttgacgttctgctgtgctccaacctgtttggcgacattctgtctgacgagtgcgcaatgatcactggctcgatggggatgttgccttccgccagcctgaacgagcaaggttttggactgtatgaaccggcgggcggctcggcaccagatatcgcaggcaaaaacatcgccaacccgattgcacaaatcctttcgctggcactgctgctgcgttacagcctggatgccgatgatgcggcttgcgccattgaacgcgccattaaccgcgcattagaagaaggcattcgcaccggggatttagcccgtggcgctgccgccgttagtaccgatgaaatgggcgatatcattgcccgctatgtagcagaaggggtgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z