LOCUS BBa_K594011 3762 bp DNA linear 22-Nov-2024 DEFINITION A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter. ACCESSION BBa_K594011 VERSION BBa_K594011.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 RBS 1071..1085 /label=RBS-1\Strong RBS 1078..1082 /label=RBS misc_feature 1071..1085 /label=BBa_B0030 RBS 1071..1085 /label=BBa_B0030 CDS 1092..1694 /label=LasI misc_feature 1695..1727 /label=LVA misc_feature 1734..1758 /label=Help:Barcodes CDS 1092..1758 /label=BBa_C0078 misc_feature 1071..1758 /label=BBa_K081009 misc_feature 1771..1774 /label=conserved RBS 1767..1778 /label=BBa_B0034 CDS 1785..2876 /label=leuB CDS 1785..2876 /label=BBa_K594002 misc_feature 2889..2892 /label=conserved RBS 2885..2896 /label=BBa_B0034 CDS 2903..3625 /label=BBa_E0030 stem_loop 3645..3688 /label=stem_loop misc_feature 3634..3713 /label=BBa_B0010 terminator 3634..3713 /label=BBa_B0010 stem_loop 3729..3748 /label=T7 TE misc_feature 3755..3755 /label=stop polyA_site 3749..3762 /label=polya misc_feature 3722..3762 /label=BBa_B0012 terminator 3722..3762 /label=BBa_B0012 terminator 3634..3762 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg 1081 agaaatacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa aaactgctgg 1141 gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg gacgtttccg 1201 ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac atgctgatcc 1261 aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac accaccggtc 1321 cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct ccgtgctccc 1381 cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt tccctgggtt 1441 tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg cagaacgaca 1501 tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc cgtgctggtc 1561 tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct gttgctctgc 1621 gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg gttgaacagc 1681 gtctggctgt ttccgctgct aacgacgaaa actacgctct ggttgcttaa taactctgat 1741 agtgctagtg tagatctcta ctagagaaag aggagaaata ctagatgtcg aagaattacc 1801 atattgccgt attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag 1861 tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag 1921 gcggcgcagc cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg 1981 agcaagccga tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac 2041 cagaccagca accagaacgc ggcgcgctgc tgcctctgcg taagcacttc aaattattca 2101 gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag 2161 acattgccgc aaacggcttc gacatcctgt gtgtgcgcga actgaccggc ggcatctatt 2221 tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga aaaagccttt gataccgagg 2281 tgtatcaccg ttttgagatc gaacgtatcg cccgcatcgc gtttgaatct gctcgcaagc 2341 gtcgccacaa agtgacgtcg atcgataaag ccaacgtgct gcaatcctct attttatggc 2401 gggagatcgt taacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca 2461 tcgacaacgc caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct 2521 ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga 2581 tgttgccttc cgccagcctg aacgagcaag gttttggact gtatgaaccg gcgggcggct 2641 cggcaccaga tatcgcaggc aaaaacatcg ccaacccgat tgcacaaatc ctttcgctgg 2701 cactgctgct gcgttacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca 2761 ttaaccgcgc attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg 2821 ttagtaccga tgaaatgggc gatatcattg cccgctatgt agcagaaggg gtgtaatact 2881 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt 2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3061 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 3121 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3481 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3601 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc 3661 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3721 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //