##gff-version 3
##sequence-region BBa_K594014 1 2790
BBa_K594014	.	engineered_region	1246	1906	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K594014	.	CDS	1264	1881	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K594014	.	tag	1843	1874	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K594014	.	engineered_region	1264	1881	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K594014	.	engineered_tag	1882	1906	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K594014	.	CDS	1264	1842	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K594014	.	ribosome_entry_site	1246	1257	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K594014	.	conserved_region	1250	1253	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K594014	.	engineered_region	1	1237	.	+	0	ID=BBa_K204032;Name=BBa_K204032
BBa_K594014	.	terminator	876	1004	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K204032
BBa_K594014	.	terminator	964	1004	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K594014	.	polyA_site	991	1004	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K594014	.	engineered_region	964	1004	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K594014	.	stem_loop	971	990	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K594014	.	stop_codon	997	997	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K594014	.	terminator	876	955	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K594014	.	engineered_region	876	955	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K594014	.	stem_loop	887	930	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K594014	.	CDS	81	867	.	+	0	ID=BBa_C0077;Name=BBa_C0077;Parent=BBa_K204032
BBa_K594014	.	engineered_tag	843	867	.	+	0	ID=annotation2214000;Name=Help:Barcodes;Parent=BBa_C0077
BBa_K594014	.	CDS	81	803	.	+	0	ID=annotation301112;Name=cinR;Parent=BBa_C0077
BBa_K594014	.	tag	804	836	.	+	0	ID=annotation306600;Name=LVA;Parent=BBa_C0077
BBa_K594014	.	promoter	1013	1237	.	+	0	ID=BBa_R0078;Name=BBa_R0078;Parent=BBa_K204032
BBa_K594014	.	sequence_feature	1013	1232	.	+	0	ID=annotation318234;Name=unidentified/uncharacterized CinR dependent;Parent=BBa_R0078
BBa_K594014	.	stem_loop	1047	1086	.	+	0	ID=annotation318244;Name=stem_loop;Parent=BBa_R0078
BBa_K594014	.	stem_loop	1103	1148	.	+	0	ID=annotation318250;Name=stem_loop;Parent=BBa_R0078
BBa_K594014	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K204032
BBa_K594014	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K594014	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K594014	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K594014	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K594014	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K594014	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K204032
BBa_K594014	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K594014	.	engineered_region	1915	2790	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K594014	.	terminator	2662	2741	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K594014	.	engineered_region	2662	2741	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K594014	.	stem_loop	2673	2716	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K594014	.	ribosome_entry_site	1915	1927	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K594014	.	ribosome_entry_site	1915	1927	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K594014	.	ribosome_entry_site	1921	1924	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K594014	.	engineered_region	1915	1927	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K594014	.	CDS	1934	2653	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K594014	.	CDS	1934	2653	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K594014	.	terminator	2750	2790	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K594014	.	polyA_site	2777	2790	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K594014	.	engineered_region	2750	2790	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K594014	.	stem_loop	2757	2776	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K594014	.	stop_codon	2783	2783	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K594014
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgcc
atccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgatt
cgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgat
cgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctg
gtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgc
tgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgc
ccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatc
gagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacac
gcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcg
catcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgcta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccctttgtgcgtcca
aacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcat
gtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaa
tttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaatt
cttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaa
aacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg
tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttg
ttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataa
ctcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaa
gatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacc
attacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaac
agctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata