LOCUS       BBa_K594015             2644 bp    DNA     linear       17-May-2024
DEFINITION  A device that can accepts the 3--O-C6-HSL and then produces lasI 
            and EYFP
ACCESSION   BBa_K594015
VERSION     BBa_K594015.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1..1062
                     /label=BBa_F2622
     RBS             1071..1085
                     /label=RBS-1\Strong
     RBS             1078..1082
                     /label=RBS
     misc_feature    1071..1085
                     /label=BBa_B0030
     RBS             1071..1085
                     /label=BBa_B0030
     CDS             1092..1694
                     /label=LasI
     misc_feature    1695..1727
                     /label=LVA
     misc_feature    1734..1758
                     /label=Help:Barcodes
     CDS             1092..1758
                     /label=BBa_C0078
     misc_feature    1071..1758
                     /label=BBa_K081009
     misc_feature    1771..1774
                     /label=conserved
     RBS             1767..1778
                     /label=BBa_B0034
     CDS             1785..2507
                     /label=BBa_E0030
     stem_loop       2527..2570
                     /label=stem_loop
     misc_feature    2516..2595
                     /label=BBa_B0010
     terminator      2516..2595
                     /label=BBa_B0010
     stem_loop       2611..2630
                     /label=T7 TE
     misc_feature    2637..2637
                     /label=stop
     polyA_site      2631..2644
                     /label=polya
     misc_feature    2604..2644
                     /label=BBa_B0012
     terminator      2604..2644
                     /label=BBa_B0012
     terminator      2516..2644
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg
     1081 agaaatacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa aaactgctgg
     1141 gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg gacgtttccg
     1201 ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac atgctgatcc
     1261 aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac accaccggtc
     1321 cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct ccgtgctccc
     1381 cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt tccctgggtt
     1441 tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg cagaacgaca
     1501 tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc cgtgctggtc
     1561 tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct gttgctctgc
     1621 gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg gttgaacagc
     1681 gtctggctgt ttccgctgct aacgacgaaa actacgctct ggttgcttaa taactctgat
     1741 agtgctagtg tagatctcta ctagagaaag aggagaaata ctagatggtg agcaagggcg
     1801 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1861 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1921 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     1981 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca
     2041 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     2101 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2161 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2221 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2281 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2341 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     2401 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2461 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac tagagccagg
     2521 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2581 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2641 tata
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