LOCUS BBa_K596004 3808 bp DNA linear 22-Dec-2024 DEFINITION Algae Protein Expression Vector ACCESSION BBa_K596004 VERSION BBa_K596004.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=suffix misc_feature 3787..3808 /label=prefix CDS 1481..2341 /label=ampR CDS 707..1326 /label=pBR322 origin promoter 2383..2411 /label=amp prom misc_feature 2490..2796 /label=f1 origin promoter 3095..3113 /label=T7 Promoter promoter 3108..3298 /label=HSP70A promoter 3299..3379 /label=RBCS2 misc_feature 3380..3778 /label=RBSC2 intron1 misc_feature 23..262 /label=3'UTR promoter 280..299 /label=T3 Promoter promoter 369..389 /label=Lac Promoter ORIGIN 1 tactagtagc ggccgctgca ggggatcccc gctccgtgta aatggaggcg ctcgttgatc 61 tgagccttgc cccctgacga acggcggtgg atggaagata ctgctctcaa gtgctgaagc 121 ggtagcttag ctccccgttt cgtgctgatc agtctttttc aacacgtaaa aagcggagga 181 gttttgcaat tttgttggtt gtaacgatcc tccgttgatt ttggcctctt tctccatggg 241 cgggctgggc gtatttgaag cgggtaccca gcttttgttc cctttagtga gggttaattg 301 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 1021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 1081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 1141 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 1201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 1261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 1321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 1381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 1441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 1501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 1561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 1621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 2041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 2101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 2161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 2221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 2281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 2341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 2401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 2461 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2521 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2581 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2641 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2701 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2761 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 2821 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 2881 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt 2941 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 3001 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 3061 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt 3121 ggagctcgct gaggcttgac atgattggtg cgtatgtttg tatgaagcta caggactgat 3181 ttggcgggct atgagggcgg gggaagctct ggaagggccg cgatggggcg cgcggcgtcc 3241 agaaggcgcc atacggcccg ctggcggcac ccatccggta taaaagcccg cgaccccgaa 3301 cggtgacctc cactttcagc gacaaacgag cacttataca tacgcgacta ttctgccgct 3361 atacataacc actcagctag cttaagatcc catcaagctt gcattccggg cgcgccagaa 3421 ggagcgcagc caaaccagga tgatgtttga tggggtattt gagcacttgc aacccttatc 3481 cggaagcccc ctggcccaca aaggctaggc gccaatgcaa gcagttcgca tgcagcccct 3541 ggagcggtgc cctcctgata aaccggccag ggggcctatg ttctttactt ttttacaaga 3601 gaagtcactc aacatcttaa aatggccagg tgagtcgacg agcaagcccg gcggatcagg 3661 cagcgtgctt gcagatttga cttgcaacgc ccgcattgtg tcgacgaagg cttttggctc 3721 ctctgtcgct gtctcaagca gcatctaacc ctgcgtcgcc gtttccattt gcaggatgct 3781 cgaggggaat tcgcggccgc ttctagag //